Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast
            <i>Saccharomyces cerevisiae</i>

Mendenhall, Michael J.; Hodge, Amy

doi:10.1128/mmbr.62.4.1191-1243.1998

Cited by 394 publications

(221 citation statements)

References 688 publications

(1,382 reference statements)

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“…In yeast, Cdc28 kinase is the central coordinator of the major events of cell division cycle promoting the transition of cells from G1 to S phase, progression through S phase, and entry into mitosis. CKS1 function is controversial but it may play an important role at the G2/M checkpoint (Mendenhall and Hodge, 1998). Other roles for the CKS1/CDC28 complex may be to enhance RNA polymerase elongation (Yu et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

The influence of follicle size, FSH‐enriched maturation medium, and early cleavage on bovine oocyte maternal mRNA levels

Mourot

Dufort

Gravel

et al. 2006

Molecular Reproduction Devel

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Transcription is arrested in the bovine oocyte within the first few hours of in vitro maturation, thus the stored maternal mRNAs accumulated in the oocyte are essential to sustain development until the Maternal-Zygotic Transition. In vivo matured oocytes have superior blastocyst formation rates than in vitro matured oocytes, suggesting that the mRNA content of these oocytes is of higher quality. To determine which transcripts may be associated with developmental competence, a Suppressive Subtractive Hybridization was performed between oocytes collected by ovariectomy at 6 hr post-LH surge and oocytes from slaughterhouse collected after 6 hr of maturation, resulting in a library enriched in these functionally important mRNAs. The clones were spotted onto a cDNA microarray and transcripts potentially associated with developmental competence were hybridized onto these slides. Hybridizations were performed with transcripts up-regulated in oocytes cultured for 6 hr in the presence or absence of rFSH in vitro, and secondly with transcripts up regulated in early-cleaving embryos versus those at the one-cell stage at 36 hr postfertilization. From these hybridizations, 13 candidates were selected. Their functional association with embryonic competence was validated by measuring their relative transcript levels by quantitative real-time PCR in eight different conditions: oocytes cultured with or without rFSH, early--versus late-cleaving embryos, and oocytes from different follicle sizes (1-3, 3-5, 5-8, and >8 mm of diameter). The gene candidates CCNB2, PTTG1, H2A, CKS1, PSMB2, SKIIP, CDC5L, RGS16, and PRDX1 showed a significant quantitative association with competence compared to BMP15, GDF9, CCNB1, and STK6.

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Section: Discussionmentioning

confidence: 99%

The influence of follicle size, FSH‐enriched maturation medium, and early cleavage on bovine oocyte maternal mRNA levels

Mourot

Dufort

Gravel

et al. 2006

Molecular Reproduction Devel

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“…Thus C. neoformans must have two critical commitment processes, one to DNA synthesis, and the other to budding. This independence between DNA synthesis and budding enables cells to continue to grow even after the cell population stops dividing, producing large unbudded G 2 cells in the stationary phase [4], in contrast to the smaller cells in S. cerevisiae [9]. Recently we reported that ploidy shift occurs frequently in C. neoformans [10].…”

Section: Discussionmentioning

confidence: 99%

Bud emergence is gradually delayed from S to G2 with progression of growth phase in Cryptococcus neoformans

Ohkusu

2001

FEMS Microbiology Letters

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The G 2 index of the yeast Cryptococcus neoformans determined by laser scanning cytometer was 2^3 times higher than the budding index during transition to the stationary phase of the culture, indicating that buds emerged in the G 2 phase of the cell cycle. To clarify whether buds also emerge in G 2 during exponential growth of the culture, DNA content for each cell was measured with a fluorescence microscope equipped with a photomultiplier. The DNA content of cells having tiny buds varied rather widely, depending on growth phases and strains used. Typically, buds of C. neoformans emerged soon after initiation of DNA synthesis in the early exponential phase. However, bud emergence was delayed to G 2 during transition to the stationary phase, and in the early stationary phase budding scarcely occurred, although roughly half of the cells completed DNA synthesis. Thus, the timing of budding in C. neoformans was actually shifted to later cell cycle points with progression of the growth phase of the culture. ß

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“…We thus expressed the Sld2-P1 protein and the Dpb11-C protein composed of the C-terminal portion of Dpb11 fused to glutathione S transferase (GST) in Escherichia coli and purified them ( Supplementary Figure 2). When we phosphorylated Sld2-P1 with recombinant Cdc28-Clb5 (Mendenhall and Hodge, 1998) (rCdc28-Clb5), the yeast S-phase-specific CDK prepared from E. coli, Sld2-P1 migrated slower than the unphosphorylated form in (Bartel and Fields, 1995). The resultant plasmids and pACT2-DPB11 (Kamimura et al, 1998) were introduced into the yeast strain L40 and the interaction was detected by lacZ expression in colour.…”

Section: A Phosphorylated 28-amino-acid Stretch Of Sld2 Binds To Dpb11mentioning

confidence: 99%

A CDK-catalysed regulatory phosphorylation for formation of the DNA replication complex Sld2–Dpb11

Tak

Takai

Endo

et al. 2006

EMBO J

100

123

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Phosphorylation often regulates protein-protein interactions to control biological reactions. The Sld2 and Dpb11 proteins of budding yeast form a phosphorylation-dependent complex that is essential for chromosomal DNA replication. The Sld2 protein has a cluster of 11 cyclindependent kinase (CDK) phosphorylation motifs (Ser/ Thr-Pro), six of which match the canonical sequences Ser/Thr-Pro-X-Lys/Arg, Lys/Arg-Ser/Thr-Pro and Ser/ Thr-Pro-Lys/Arg. Simultaneous alanine substitution for serine or threonine in all the canonical CDK-phosphorylation motifs severely reduces complex formation between Sld2 and Dpb11, and inhibits DNA replication. Here we show that phosphorylation of these canonical motifs does not play a direct role in complex formation, but rather regulates phosphorylation of another residue, Thr84. This constitutes a non-canonical CDK-phosphorylation motif within a 28-amino-acid sequence that is responsible, after phosphorylation, for binding of Sld2-Dpb11. We further suggest that CDK-catalysed phosphorylation of sites other than Thr84 renders Thr84 accessible to CDK. Finally, we argue that this novel mechanism sets a threshold of CDK activity for formation of the essential Sld2 to Dpb11 complex and therefore prevents premature DNA replication.

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Regulation of Cdc28 Cyclin-Dependent Protein Kinase Activity during the Cell Cycle of the Yeast Saccharomyces cerevisiae

Cited by 394 publications

References 688 publications

The influence of follicle size, FSH‐enriched maturation medium, and early cleavage on bovine oocyte maternal mRNA levels

The influence of follicle size, FSH‐enriched maturation medium, and early cleavage on bovine oocyte maternal mRNA levels

Bud emergence is gradually delayed from S to G2 with progression of growth phase in Cryptococcus neoformans

A CDK-catalysed regulatory phosphorylation for formation of the DNA replication complex Sld2–Dpb11

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