Regulation of cell differentiation to promote pullulan synthesis in Aureobasidium pullulans NG

Zeng, Nan; Zhang, Ning; Wang, Dandan; Long, Jiajia; Wang, Yunjiao; Zhang, Yating; Pu, Fangxiong; Li, Zijing; Baloch, Faryal Babar; Li, Bingxue

doi:10.1007/s00253-023-12758-z

Cited by 4 publications

(2 citation statements)

References 53 publications

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“…These changes in cell differentiation and vacuole characteristics were likely influenced by variations in nutrients and pH values, impacting pullulan synthesis. Swollen cells emerged as a prominent cell type during pullulan synthesis in A. pullulans NG [ 40 ], exhibiting a robust stress defense and antioxidative capabilities compared with yeast-like cells [ 22 ]. As shown in Figure 5 , swollen cells under the OC seemed to have a stronger and more stable ability to withstand environmental stress, which may require for high levels of pullulan production.…”

Section: Discussionmentioning

confidence: 99%

“…It was found that A. pullulans NG grew well under the nutrient-rich condition but produced mycelia and chlamydospores under the nutrient-poor condition; while the osmotic stress prevented the differentiation of yeast-like cells into two types of the above-mentioned cells [41]. Both one type of cells (e.g., yeast-like cells [13], swollen cells [40], or arthroconidia [18]) and two types of cells (e.g., yeast-like cells and swollen cells) [9] were conducive to pullulan synthesis. However, the exact cell type responsible for pullulan synthesis in Aureobasidium strains remains unclear [1].…”

Section: Robust Pullulan Synthesis By Strain Zh27 After Optimizationmentioning

confidence: 99%

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Hyper-Production of Pullulan by a Novel Fungus of Aureobasidium melanogenum ZH27 through Batch Fermentation

Wang,

Lin,

Zhou

et al. 2023

IJMS

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Pullulan, which is a microbial exopolysaccharide, has found widespread applications in foods, biomedicines, and cosmetics. Despite its versatility, most wild-type strains tend to yield low levels of pullulan production, and their mutants present genetic instability, achieving a limited increase in pullulan production. Therefore, mining new wild strains with robust pullulan-producing abilities remains an urgent concern. In this study, we found a novel strain, namely, Aureobasidium melanogenum ZH27, that had a remarkable pullulan-producing capacity and optimized its cultivation conditions using the one-factor-at-a-time method. To elucidate the reasons that drove the hyper-production of pullulan, we scrutinized changes in cell morphology and gene expressions. The results reveal that strain ZH27 achieved 115.4 ± 1.82 g/L pullulan with a productivity of 0.87 g/L/h during batch fermentation within 132 h under the optimized condition (OC). This pullulan titer increased by 105% compared with the initial condition (IC). Intriguingly, under the OC, swollen cells featuring 1–2 large vacuoles predominated during a rapid pullulan accumulation, while these swollen cells with one large vacuole and several smaller ones were prevalent under the IC. Moreover, the expressions of genes associated with pullulan accumulation and by-product synthesis were almost all upregulated. These findings suggest that swollen cells and large vacuoles may play pivotal roles in the high level of pullulan production, and the accumulation of by-products also potentially contributes to pullulan synthesis. This study provides a novel and promising candidate for industrial pullulan production.

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Section: Discussionmentioning

confidence: 99%

Section: Robust Pullulan Synthesis By Strain Zh27 After Optimizationmentioning

confidence: 99%

Hyper-Production of Pullulan by a Novel Fungus of Aureobasidium melanogenum ZH27 through Batch Fermentation

Wang,

Lin,

Zhou

et al. 2023

IJMS

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Role of AplaeA in the regulation of spore production and poly(malic acid) synthesis in Aureobasidium pullulans

Guan,

Wang,

Zhang

et al. 2024

International Journal of Biological Macromolecules

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Transcriptome and metabolome integration analysis reveals the effect of citric acid on cell metabolism in Aureobasidium pullulans NG

Zeng,

Wang,

Long

et al. 2025

LWT

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Regulation of cell differentiation to promote pullulan synthesis in Aureobasidium pullulans NG

Cited by 4 publications

References 53 publications

Hyper-Production of Pullulan by a Novel Fungus of Aureobasidium melanogenum ZH27 through Batch Fermentation

Hyper-Production of Pullulan by a Novel Fungus of Aureobasidium melanogenum ZH27 through Batch Fermentation

Role of AplaeA in the regulation of spore production and poly(malic acid) synthesis in Aureobasidium pullulans

Transcriptome and metabolome integration analysis reveals the effect of citric acid on cell metabolism in Aureobasidium pullulans NG

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