Regulation of cell size and cell number by LANCEOLATA1 gene in Arabidopsis

Cho, Kiu-Hyung; Jun, Sang Eun; Jeong, Soon-Jae; Yi, Young-Byung; Kim, Gyung-Tae

doi:10.5352/jls.2007.17.1.001

Cited by 1 publication

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“…잎 발달 동 안의 heteroblasty는 외부 환경에 대한 식물의 형태적 적응 방법과 그에 따른 기관 내 세포의 증식 및 확장 (cell expansion)을 매우 잘 반영한다 . 결국, leaf index 등의 잎 기관의 형태 및 모양의 변화는 잎몸에 서의 세포 증식과 세포 확장의 두 요인에 의해 결정된다 (Arkebuer and Norman 1995;Cho et al 2007 (Fig. 2).…”

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MACROPHYLLA/ROTUNDIFOLIA3 gene of Arabidopsis controls leaf index during leaf development

Jun¹,

Chandrasekhar²,

Cho³

et al. 2011

Journal of Plant Biotechnology

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In plants, heteroblasty reflects the morphological adaptation during leaf development according to the external environmental condition and affects the final shape and size of organ. Among parameters displaying heteroblasty, leaf index is an important and typical one to represent the shape and size of simple leaves. Leaf index factor is eventually determined by cell proliferation and cell expansion in leaf blades. Although several regulators and their mechanisms controlling the cell division and cell expansion in leaf development have been studied, it does not fully provide a blueprint of organ formation and morphogenesis during environmental changes. To investigate genes and their mechanisms controlling leaf index during leaf development, we carried out molecular-genetic and physiological experiments using an Arabidopsis mutant. In this study, we identified macrophylla (mac) which had enlarged leaves. In detail, the mac mutant showed alteration in leaf index and cell expansion in direction of width and length, resulting in not only modification of leaf shape but also disruption of heteroblasty. Molecular-genetic studies indicated that mac mutant had point mutation in ROTUDIFOLIA3 (ROT3) gene involved in brassinosteroid biosynthesis and was an allele of rot3-1 mutant. We named it mac/rot3-5 mutant. The expression of ROT3 gene was controlled by negative feedback inhibition by the treatment of brassinosteroid hormone, suggesting that ROT3 gene was involved in brassinosteroid biosynthesis. In dark condition, in addition, the expression of ROT3 gene was up-regulated and mac/rot3-5 mutant showed lower response, compare to wild type in petiole elongation. This study suggests that ROT3 gene has an important role in control of leaf index during leaf expansion process for proper environmental adaptation, such as shade avoidance syndrome, via the control of brassinosteroid biosynthesis.

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