Regulation of Circulatory Muscle-specific MicroRNA during 8 km
                    Run

Yin, Xin; Cui, Shufang; Li, Xin; Li, Wei; Lu, Qiu Ju; Jiang, Xiao hong; Wang, Hui; Chen, Xi; Zheng, Ji

doi:10.1055/a-1145-3595

Cited by 12 publications

(8 citation statements)

References 33 publications

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“…Moreover, the nature and magnitude of the responses of the circulating miRNAs were influenced by exercise type, which likely depends on exercise intensity or volume. [24][25][26] Thus, we hypothesized that exercise may also help to inhibit SARS-CoV-2 replication through regulating circulating miRNAs profiles. To investigate whether exercise can improve the ability to resist SARS-CoV-2, we first measured the levels of the four circulating miRNAs in individual serum samples from healthy volunteers with or without 8-weeks' continuous physical exercise.…”

Section: Resultsmentioning

confidence: 99%

Decreased inhibition of exosomal miRNAs on SARS-CoV-2 replication underlies poor outcomes in elderly people and diabetic patients

Zhu

Jiang

et al. 2021

Sig Transduct Target Ther

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Elderly people and patients with comorbidities are at higher risk of COVID-19 infection, resulting in severe complications and high mortality. However, the underlying mechanisms are unclear. In this study, we investigate whether miRNAs in serum exosomes can exert antiviral functions and affect the response to COVID-19 in the elderly and people with diabetes. First, we identified four miRNAs (miR-7-5p, miR-24-3p, miR-145-5p and miR-223-3p) through high-throughput sequencing and quantitative real-time PCR analysis, that are remarkably decreased in the elderly and diabetic groups. We further demonstrated that these miRNAs, either in the exosome or in the free form, can directly inhibit S protein expression and SARS-CoV-2 replication. Serum exosomes from young people can inhibit SARS-CoV-2 replication and S protein expression, while the inhibitory effect is markedly decreased in the elderly and diabetic patients. Moreover, three out of the four circulating miRNAs are significantly increased in the serum of healthy volunteers after 8-weeks’ continuous physical exercise. Serum exosomes isolated from these volunteers also showed stronger inhibitory effects on S protein expression and SARS-CoV-2 replication. Our study demonstrates for the first time that circulating exosomal miRNAs can directly inhibit SARS-CoV-2 replication and may provide a possible explanation for the difference in response to COVID-19 between young people and the elderly or people with comorbidities.

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Section: Resultsmentioning

confidence: 99%

Decreased inhibition of exosomal miRNAs on SARS-CoV-2 replication underlies poor outcomes in elderly people and diabetic patients

Zhu

Jiang

et al. 2021

Sig Transduct Target Ther

Self Cite

View full text Add to dashboard Cite

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“…Moreover, miR-133a-3p was still elevated after 24 h of rest, whereas miR-1-3p and miR-133b-3p returned to their baseline. Levels of miR-1-3p and miR-133a-3p were correlated with myoglobin at 24 h after the run [ 39 ]. Baggish et al showed that the concentration of cardiac-tissue-specific circulating miR-208a was significantly upregulated before, but it was substantially decreased 24 h after completing the marathon.…”

Section: Resultsmentioning

confidence: 99%

MicroRNAs as Biomarkers of Systemic Changes in Response to Endurance Exercise—A Comprehensive Review

et al. 2020

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Endurance sports have an unarguably beneficial influence on cardiovascular health and general fitness. Regular physical activity is considered one of the most powerful tools in the prevention of cardiovascular disease. MicroRNAs are small particles that regulate the post-transcription gene expression. Previous studies have shown that miRNAs might be promising biomarkers of the systemic changes in response to exercise, before they can be detected by standard imaging or laboratory methods. In this review, we focused on four important physiological processes involved in adaptive changes to various endurance exercises (namely, cardiac hypertrophy, cardiac myocyte damage, fibrosis, and inflammation). Moreover, we discussed miRNAs’ correlation with cardiopulmonary fitness parameter (VO2max). After a detailed literature search, we found that miR-1, miR-133, miR-21, and miR-155 are crucial in adaptive response to exercise.

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“…However, specific circulating miR-1 was significantly up-regulated 3 h post exercise but not immediately or shortly after (within <1 h), which concords with our findings as blood samples for analysis were obtained within 30 min after finishing the race ( Nielsen et al, 2014 ; Denham et al, 2018 ). We previously described that several studies found elevated miR-1-3p in response to endurance training, however, only one study correlated this finding with myoglobin at 24 h after the run ( Soplinska et al, 2020 ; Yin et al, 2020 ). Moreover, in the current study we found a negative association between miR-1-3p and CRP levels after the 100 km race.…”

Section: Discussionmentioning

confidence: 93%

Altered Circulating MicroRNA Profiles After Endurance Training: A Cohort Study of Ultramarathon Runners

et al. 2022

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BackgroundDespite the positive effects of endurance training on the cardiovascular (CV) system, excessive exercise induces not only physiological adaptations but also adverse changes in CV system, including the heart. We aimed to evaluate the selected miRNAs expression based on bioinformatic analysis and their changes before and after an ultramarathon run.Materials and MethodsCardiac tissue-specific targets were identified with the Tissue 2.0 database. Gene-gene interaction data were retrieved from the STRING app for Cytoscape. Twenty-three endurance athletes were recruited to the study. Athletes ran to completion (100 km) or exhaustion (52–91 km, median 74 km). All participants completed pre- and post-run testing. miRNAs expressions were measured both before and after the race.ResultsEnrichment analysis of the signaling pathways associated with the genes targeted by miRNAs selected for qRT-PCR validation (miR-1-3p, miR-126, miR-223, miR-125a-5p, miR-106a-5p, and miR-15a/b). All selected miRNAs showed overlap in regulation in pathways associated with cancer, IL-2 signaling, TGF-β signaling as well as BDNF signaling pathway. Analysis of metabolites revealed significant regulation of magnesium and guanosine triphosphate across analyzed miRNA targets. MiR-1-3p, miR-125a-5p, miR-126, and miR-223 expressions were measured in 23 experienced endurance athletes, before and after an ultramarathon wherein athletes ran to completion (100 km) or exhaustion (52–91 km, median 74 km). The expressions of miR-125a-5p, miR-126, and miR-223 were significantly increased after the race (p = 0.007, p = 0.001, p = 0.014, respectively). MiR-1-3p expression post-run showed a negative correlation with the post-run levels of high-sensitivity C-reactive protein (hs-CRP) (r = −0.632, p = 0.003). Higher miR-1-3p expression was found in runners, who finished the race under 10 h compared to runners who finished over 10 h (p = 0.001). Post-run miR-125a-5p expression showed a negative correlation with the peak lactate during the run (r = −0.576, p = 0.019).ConclusionExtreme physical activity, as exemplified by an ultramarathon, is associated with changes in circulating miRNAs’ expression related to inflammation, fibrosis, and cardiac muscle function. In particular, the negative correlations between miR-125a-5p and lactate concentrations, and miR-1-3p and hs-CRP, support their role in specific exercise-induced adaptation. Further studies are essential to validate the long-term effect of these observations.

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Regulation of Circulatory Muscle-specific MicroRNA during 8 km Run

Cited by 12 publications

References 33 publications

Decreased inhibition of exosomal miRNAs on SARS-CoV-2 replication underlies poor outcomes in elderly people and diabetic patients

Decreased inhibition of exosomal miRNAs on SARS-CoV-2 replication underlies poor outcomes in elderly people and diabetic patients

MicroRNAs as Biomarkers of Systemic Changes in Response to Endurance Exercise—A Comprehensive Review

Altered Circulating MicroRNA Profiles After Endurance Training: A Cohort Study of Ultramarathon Runners

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