Regulation of connexin 43 by nitric oxide in primary uterine myocytes from term pregnant women

Roh, Cheong‐Rae; Heo, Ji-Hee; Yang, Soon-Ha; Bae, Duk-Soo

doi:10.1067/mob.2002.123600

Cited by 30 publications

(23 citation statements)

References 24 publications

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“…The discrepancy of the results from uterine myocytes and glomerular MC may be due to the different properties of the cells used for study. Contrary to most of the previous observations (18,19), cAMP has been shown to inhibit Cx43 expression in myocytes (37). In a recent study using human umbilical vein endothelial cells, NO was shown to enhance endothelial cell coupling and selectively increase the expression of Cx40 but not Cx43 (12).…”

Section: Discussionmentioning

confidence: 39%

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Nitric Oxide-Mediated Regulation of Connexin43 Expression and Gap Junctional Intercellular Communication in Mesangial Cells

Yao¹,

Hiramatsu²,

Zhu

et al. 2005

Journal of the American Society of Nephrology

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Section: Discussionmentioning

confidence: 39%

“…Sladek et al (36) showed that endogenous NO attenuated myometrial Cx43 expression during rat pregnancy. Another report, by Roh et al (37), also demonstrated an inhibitory effect of exogenous NO on Cx43 levels in cultured uterine myocytes. The mechanisms involved are currently unknown.…”

Section: Discussionmentioning

confidence: 91%

Nitric Oxide-Mediated Regulation of Connexin43 Expression and Gap Junctional Intercellular Communication in Mesangial Cells

Yao¹,

Hiramatsu²,

Zhu

et al. 2005

Journal of the American Society of Nephrology

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“…NO, a free radical synthesized by a family of enzymes known as NOS, is an important physiological regulator of uterine contractility (22). NO inhibits uterine contractility by various mechanisms, including the reduction of intracellular calcium levels via protein kinase C, activation of calcium pumps, and suppression of the expression of gap junctions consisting of proteins called connexin 43 (Cx43), resulting in the facilitation of action potential propagation from one cell to another (23)(24)(25). Endogenous metabolism of NO gives rise to plasma and urinary nitrite (NO 2 ) and nitrate (NO 3 ).…”

Section: Discussionmentioning

confidence: 99%

Glyceryl trinitrate for the treatment of preterm labor

Çalışkan

Narin

Dede

et al. 2015

J Turkish German Gynecol Assoc

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Results: Forty-one patients were included into the final analysis. Uterine contraction cessation times were 3.66±1.28 and 6.83±3.47 hours for GTN and MgSO4 groups, respectively. Similarly, maternal side effects were significantly lower in the GTN group than in the MgSO4 group, and no serious maternal side effects were recorded. Serum NO metabolite levels before treatment were significantly lower in the treatment groups than in the controls. Serum nitrite levels were significantly increased after tocolytic treatment both in MgSO 4 and GTN groups.Conclusion: GTN effectively delays preterm delivery and reduces neonatal morbidity and mortality with less maternal side effects and seems to be an effective and safe alternative to MgSO 4 . (J Turk Ger Gynecol Assoc 2015; 16: 174-8) Keywords: Glyceryl trinitrate, nitric oxide, magnesium sulfate, tocolysis Received: 28 January, 2015 Accepted: 24 March, 2015 Available Online Date: 14 July, 2015 Glyceryl trinitrate for the treatment of preterm labor Obstetrics and Gynecology, Zekai Tahir Burak Training and Research Hospital, Ankara, Turkey Abstract with intact amniotic membrane, reassuring fetal heart rate (FHR) tracing, and no contraindication for tocolysis. Exclusion criteria included multiple pregnancy, nonreassuring FHR tracing, hypotension (blood pleasure <80/50 mmHg), unexplained vaginal bleeding, placenta previa, preterm premature rupture of membranes, chorioamnionitis, cervical cerclage during present gestation, urinary system infection, maternal systemic disease, fetal growth restriction, and sensitivity or contraindication to nitrates or magnesium sulfate. Eligible women were then approached to participate in the trial, and after final recruitment, the study protocol was explained to the patients and informed consents were obtained. The patients were randomized into two treatment groups according to software-generated random allocation sequence. The random allocation sequence was held by only one author during the entire trial period. The tocolytic drug was changed in four patients from the MgSO 4 group and in three patients from GTN group, and these patients were excluded from the study in accordance with the study design ( Figure 1). In conclusion, 19 patients in the MgSO 4 group and 22 patients in GTN group were included in the final analysis. All the groups were matched with regard to maternal age, gestational age, and parity. Study designAt the time of initial assessment, blood samples were collected for routine tests and serum nitrate and nitrite levels, following which maternal sedation with 10 mg intramuscular (IM) diazepam (Diazem ® , Deva, Turkey) and hydration with 500 mL Ringer's lactate and with 500 mL 5% dextrose solution were performed. Betamethasone (Celestone Chronodose ® , Schering Plough, Turkey) was administered at 12 mg IM every 24 hours for a total of two doses to all the patients for promoting fetal lung development. Patients could not be treated with sedation and hydration underwent MgSO 4 or GTN treatment. MgSO 4 was performe...

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“…In vitro studies have shown that exogenous nitric oxide (NO) increased the expression of Cx40 in endothelial gap junctions (Hoffmann et al, 2003) and Cx43 expression in mesangial cells (Yao et al, 2005) but decreased Cx43 expression in uterine myocytes (Sladek et al, 1999;Roh et al, 2002). Elevated glucose has also been shown to inhibit gap junctional communication in cultured smooth muscle cells through alterations to the phosphorylation of Cx43 (Kuroki et al, 1998) and in cultured microvascular endothelial cells through downregulation of Cx43 expression (Sato et al, 2002).…”

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confidence: 99%

Increased eNOS accounts for changes in connexin expression in renal arterioles during diabetes

et al. 2006

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Previous studies have shown that connexin (Cx) expression is considerably higher in the preglomerular compared to postglomerular vasculature and that these differences are accentuated during diabetes. Since nitric oxide (NO) has been reported to alter Cx expression in endothelial cells and muscle cells and NO bioavailability is altered in diabetes, we hypothesized that NO may be responsible for the changes during diabetes. Cx expression was studied using immunohistochemistry in mice in which eNOS expression was either upregulated (eNOS transgenic) or downregulated (eNOS knockout). Diabetes was induced intraperitoneally with a single dose of alloxan or multiple low doses of streptozotocin. Expression of Cx40 in smooth muscle cells of afferent arterioles was increased, while expression of Cx43 in endothelial cells of efferent arterioles was absent in eNOS transgenic mice, similar to the changes occurring in wild-type mice during diabetes. Expression of Cx40 and Cx43 in eNOS knockout mice was not different from control; however, induction of diabetes in eNOS knockout mice failed to produce any changes in Cx40 or Cx43 in either afferent or efferent arterioles. Immunohistochemistry showed that eNOS expression was increased in the endothelium of renal arterioles in wild-type diabetic and eNOS transgenic mice, but absent from arterioles of eNOS knockout mice. We conclude that changes occurring in Cx expression in afferent and efferent arterioles during diabetes may result from increased eNOS.

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Regulation of connexin 43 by nitric oxide in primary uterine myocytes from term pregnant women

Cited by 30 publications

References 24 publications

Nitric Oxide-Mediated Regulation of Connexin43 Expression and Gap Junctional Intercellular Communication in Mesangial Cells

Nitric Oxide-Mediated Regulation of Connexin43 Expression and Gap Junctional Intercellular Communication in Mesangial Cells

Glyceryl trinitrate for the treatment of preterm labor

Increased eNOS accounts for changes in connexin expression in renal arterioles during diabetes

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