Regulation of Connexin31 Gene Expression upon Retinoic Acid Treatment in Rat Choriocarcinoma Cells

Expression of Cathepsin P mRNA, Protein and Activity in the Rat Choriocarcinoma Cell Line, Rcho-1, During Giant Cell Transformation

“…After 10 days in differentiation media, cells begin to die, showing reduced levels of Hand1 and Stra13 as well as control β-actin ( fig 1C). These results are consistent with previous studies that have shown induction of these transcription factors during differentiation of mouse trophoblast stem cells into trophoblast giant cells [14,[27][28][29].…”

“…Many studies have validated this cell model for studying aspects of trophoblast biology including cell cycle regulation, cell differentiation regulation, trophoblast phenotypic characterization, cell invasion, and trophoblast tumor development [14,16,24,[30][31][32][33][34][35]. Differentiation of Rcho-1 cells to yield cells with a trophoblast giant cell phenotype helps overcome the inherent difficulties in obtaining purified populations of these cells from tissues [16].…”

“…A wellestablished cell model of trophoblast cell differentiation is Rcho-1, a rat choriocarcinoma cell line that can be manipulated in culture to differentiate into cells that resemble trophoblast giant cells (TGs) [14][15][16]. The cells become polyploid due to multiple rounds of DNA synthesis without mitosis (endoreduplication).…”

Expression of connexin31 and connexin43 genes in early rat embryos

“…blastocyst, mRNA was present. For control purpose, total RNA of the rat choriocarcinoma cell line, RCHO-1 [Faria and Soares, 1991], expressing Cx31 and of the Holtzman rat spontaneously immortalized placenta cell line HRP [Soares et al, 1987] expressing Cx43 was used [Grü mmer et al, 1996b]. For the differences in the time course between whole-mount in situ hybridization and RT-PCR, we used a primer specific for Cx31 for RT.…”

SV40 Tag transformation of the normal invasive trophoblast results in a premalignant phenotype. II. Changes in gap junctional intercellular communication

“…Gap junctions are expressed by trophoblast cells of the invasive, hemochorial placenta in numerous species e.g., mice (Dahl et al, 1995;Pauken and Lo, 1995), rats (Risek and Gilula, 1991;Winterhager et al, 1996b;Grummer et al, 1996), and humans (Cronier et al, 1995(Cronier et al, , 1997Hellmann et al, 1995Hellmann et al, , 1996. Shortly after implantation of rat embryos, invasive ectoplacental cone trophoblast cells express Cx31, which later disappears with further trophoblast differentiation; Cx26 appears in the labyrinthine trophoblast whereas Cx43 appears in the spongiotrophoblast and trophoblast giant cell layers that invade the decidua (Risek and Gilula, 1991;Reuss et al, 1996).…”