Regulation of Endometrial Responsiveness to Estrogen and Progesterone by Pregnancy Recognition Signals During the Periimplantation Period

Bazer, Fuller W.; Spencer, Thomas E.; Ott, Troy L.; Ing, Nancy H.

doi:10.1007/978-1-4612-2548-5_3

Cited by 11 publications

(10 citation statements)

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“…Sheep conceptuses begin to produce high levels of IFN between D12-14 postmating (Bazer et al 1995). Results from the present study indicate that Mx gene expression increases 4-5 fold in PBMC within 24-48 h of initial IFN signaling (Bazer et al 1995). This systemic response to IFN continued through D30 post-AI; 8-10 days after conceptus production of IFN is detectable (Farin et al 1989).…”

Section: Discussionmentioning

confidence: 61%

“…In addition, Mx expression did not increase in PBMC when pregnancy was not established (bred, non-pregnant ewes). These findings are novel because pregnancy recognition signaling by IFN was heretofore considered to result solely from local regulation of endometrial gene expression Johnson et al 2001;Vallet et al 1991;Spencer et al 1998;Johnson et al 2000;Charleston & Stewart 1993;Ott et al 1998;Spencer et al 1999) and suppression of estrogen and oxytocin receptor expression to abrogate luteolytic pulses of PGF 2 (Spencer et al 1995). Sheep conceptuses begin to produce high levels of IFN between D12-14 postmating (Bazer et al 1995).…”

Section: Discussionmentioning

confidence: 95%

“…Pregnancy recognition in domestic ungulates involves local regulation of endometrial gene expression by the conceptus that results in reduced or altered production of the luteolytic signal, prostaglandin F 2 (PGF 2 ; Bazer et al 1995). This is in contrast to pregnancy recognition in primates, which involves a direct luteotrophic effect on the corpus luteum (CL) by conceptus-produced chorionic gonadotropin (Bazer et al 1995).…”

Section: Introductionmentioning

confidence: 84%

“…This is in contrast to pregnancy recognition in primates, which involves a direct luteotrophic effect on the corpus luteum (CL) by conceptus-produced chorionic gonadotropin (Bazer et al 1995). Ruminant conceptuses secrete IFN , the signal for pregnancy recognition, during the second and third week of pregnancy (Bazer et al 1995;Godkin et al 1982). IFN prevents increases in endometrial estrogen and oxytocin receptors, to abrogate oxytocin-induced luteolytic pulses of PGF 2 , and maintains CL function (Spencer et al 1995).…”

Section: Introductionmentioning

confidence: 98%

“…Ruminant conceptuses secrete IFN , the signal for pregnancy recognition, during the second and third week of pregnancy (Bazer et al 1995;Godkin et al 1982). IFN prevents increases in endometrial estrogen and oxytocin receptors, to abrogate oxytocin-induced luteolytic pulses of PGF 2 , and maintains CL function (Spencer et al 1995).…”

Section: Introductionmentioning

confidence: 99%

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Expression of the antiviral protein Mx in peripheral blood mononuclear cells of pregnant and bred, non-pregnant ewes

Yankey

Hicks²,

Carnahan³

et al. 2001

Journal of Endocrinology

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Interferon-tau (IFN ) acts locally on the endometrium to suppress estrogen and oxytocin receptor expression and block luteolysis in ruminants. Systemic administration of conceptus homogenates or recombinant ovine IFN does not block luteolysis or enhance pregnancy rates in sheep or cattle, respectively. However, IFN up-regulates expression of the antiviral protein Mx throughout the entire uterine wall during early pregnancy. These studies determined if conceptus-derived IFN also up-regulates Mx expression in components of the circulating immune system that migrate through the endometrial wall. In experiment one, peripheral blood mononuclear cells (PBMC) were isolated from ewes at D26 post-artificial insemination (AI) and Mx mRNA levels examined by Northern and slot-blot hybridization. Pregnancy resulted in a two-fold increase in Mx mRNA levels compared to bred, non-pregnant ewes at D26. In experiment two, PBMC were isolated from ewes at AI, and every three days from D9 to D30. Results showed a four-fold increase in Mx mRNA levels in PBMC from pregnant versus bred, non-pregnant ewes at D15. Increased Mx mRNA, which remained elevated through D30, was accompanied by increased levels of Mx protein. These results show that pregnancy recognition signaling rapidly induces Mx gene expression in PBMC, and are the first to suggest that IFN activates gene expression in components of the circulating immune system.

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Section: Discussionmentioning

confidence: 61%