Regulation of fatty acid and flavonoid biosynthesis by miRNAs in Lonicera japonica

Liu, Juan; Yuan, Yuan; Wang, Yaolong; Chen, Ti-Ying; Zhu, Feng-Jie; Zhao, Yang; Zhou, Junhui; Huang, Luqi

doi:10.1039/c7ra05800d

Cited by 25 publications

(18 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No significant PKS and NRPS gene upregulation was observed under nitrogen starvation, in which miRNA-mediated post-transcriptional regulation might affect secondary metabolism by targeting pyruvate biosynthesis. miRNA effects on secondary metabolite biosynthesis have been reported in plants [47,48].…”

Section: Possible Regulation Of Toxin Biosynthesis By Micrornas Durinmentioning

confidence: 99%

Integrated omics unveil the secondary metabolic landscape of a basal dinoflagellate

et al. 2020

View full text Add to dashboard Cite

Background Some dinoflagellates cause harmful algal blooms, releasing toxic secondary metabolites, to the detriment of marine ecosystems and human health. Our understanding of dinoflagellate toxin biosynthesis has been hampered by their unusually large genomes. To overcome this challenge, for the first time, we sequenced the genome, microRNAs, and mRNA isoforms of a basal dinoflagellate, Amphidinium gibbosum, and employed an integrated omics approach to understand its secondary metabolite biosynthesis. Results We assembled the ~ 6.4-Gb A. gibbosum genome, and by probing decoded dinoflagellate genomes and transcriptomes, we identified the non-ribosomal peptide synthetase adenylation domain as essential for generation of specialized metabolites. Upon starving the cells of phosphate and nitrogen, we observed pronounced shifts in metabolite biosynthesis, suggestive of post-transcriptional regulation by microRNAs. Using Iso-Seq and RNA-seq data, we found that alternative splicing and polycistronic expression generate different transcripts for secondary metabolism. Conclusions Our genomic findings suggest intricate integration of various metabolic enzymes that function iteratively to synthesize metabolites, providing mechanistic insights into how dinoflagellates synthesize secondary metabolites, depending upon nutrient availability. This study provides insights into toxin production associated with dinoflagellate blooms. The genome of this basal dinoflagellate provides important clues about dinoflagellate evolution and overcomes the large genome size, which has been a challenge previously.

show abstract

Section: Possible Regulation Of Toxin Biosynthesis By Micrornas Durinmentioning

confidence: 99%

Integrated omics unveil the secondary metabolic landscape of a basal dinoflagellate

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Phenylpropanoid 4-coumaroil-CoA is the precursor for the synthesis of flavonols and anthocyanins [ 110 ]. Flavonoid biosynthesis is highly regulated by miRNAs [ 111 , 112 , 113 ], in which interactions of different families of transcription factors that participate in the various branches of this route are observed in a modular way, which include members of the R2R3 family of MYB transcription factors ( AtMYB11 , AtMYB12 , and AtMYB111 ), basic helix-loop-helix (bHLH), and WD40 [ 114 ]. In Arabidopsis , miR858a regulates the activity of several MYB transcription factors that play a role in the flavonoid biosynthesis pathway.…”

Section: Mirnas and Secondary Metabolites In The Defense Response mentioning

confidence: 99%

The Critical Role of Small RNAs in Regulating Plant Innate Immunity

et al. 2021

View full text Add to dashboard Cite

Plants, due to their sessile nature, have an innate immune system that helps them to defend against different pathogen infections. The defense response of plants is composed of a highly regulated and complex molecular network, involving the extensive reprogramming of gene expression during the presence of pathogenic molecular signatures. Plants attain proper defense against pathogens through the transcriptional regulation of genes encoding defense regulatory proteins and hormone signaling pathways. Small RNAs are emerging as versatile regulators of plant development and act in different tiers of plant immunity, including pathogen-triggered immunity (PTI) and effector-triggered immunity (ETI). The versatile regulatory functions of small RNAs in plant growth and development and response to biotic and abiotic stresses have been widely studied in recent years. However, available information regarding the contribution of small RNAs in plant immunity against pathogens is more limited. This review article will focus on the role of small RNAs in innate immunity in plants.

show abstract

“…These molecules play an important role in posttranscriptional regulation by the inhibition of the expression of target genes by binding to mRNA [1]. Eukaryotic organisms, such as plants and animals, and some viruses express this type of molecules [2,3]. Lin-4 is the first miRNA that was identified in Caenorhabditis elegans, although later in the same organism were found 22-nt lin-4 and 21-nt let-7; currently, more than 18,226 miRNAs are reported [4].…”

Section: Definition Of Mirnamentioning

confidence: 99%

“…miR395 is another miRNA that has an influence on PA biosynthesis [30]. Some miRNAs (U436803, U977315, U805963, U3938865, and U4351355) regulate fatty acid and flavonoid biosynthesis in Lonicera japonica [2,3]. The characterization in A. thaliana shows that miR858a targets MYB transcription factors that are involved in flavonoid biosynthesis, growth, and development.…”

Section: Flavonoidsmentioning

confidence: 99%

MicroRNAs Associated with Secondary Metabolites Production

Vargas-Hernández¹,

Vázquez-Marrufo²,

Agustín³

et al. 2019

Plant Physiological Aspects of Phenolic Compounds

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are noncoding RNAs that play an important role in the regulation of the genetic expression in animals and plants by targeting mRNAs for cleavage or translational repression. Several miRNAs regulate the plant development, the metabolism, and the responses to biotic and abiotic stresses. Characterization of an miRNA has helped to show its role in fine tuning the mechanisms of posttranscriptional gene regulation. Although there is a lot of information related to miRNA regulation of some processes, the role of miRNA involved in the regulation of biosynthesis of secondary plant product is still poorly understood. In this chapter, we summarize the identification and characterization of miRNAs that participate in the regulation of the biosynthesis of secondary metabolites in plants and their use in the strategies to manipulate a controlled manipulation.

show abstract

Regulation of fatty acid and flavonoid biosynthesis by miRNAs in Lonicera japonica

Abstract: This is the first report on miRNAs in different varieties of honeysuckle, the results of which can further facilitate the discovery of functional regulatory miRNAs involved in the secondary metabolism in L. japonica.

Cited by 25 publications

References 32 publications

Integrated omics unveil the secondary metabolic landscape of a basal dinoflagellate

Integrated omics unveil the secondary metabolic landscape of a basal dinoflagellate

The Critical Role of Small RNAs in Regulating Plant Innate Immunity

MicroRNAs Associated with Secondary Metabolites Production

Contact Info

Product

Resources

About