Regulation of fatty acid biosynthesis in Escherichia coli.

Magnuson, Kelly S.; Jackowski, Suzanne; Rock, Charles O.; Cronan, John E.

doi:10.1128/mmbr.57.3.522-542.1993

Cited by 378 publications

(287 citation statements)

References 71 publications

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“…The situation of plsX and fabH in the same region as rpmF suggests a linkage between ribosomal protein synthesis and membrane lipid biosynthesis. In R. capsulatus, these genes are directly upstream of the gene himA, encoding the a subunit of integration host factor (IHF) [6]; in E. coli, rpmF plsX fabH are upstream of additional lab genes (fabD fabG acpP fabF) [16,17].…”

Section: Discussionmentioning

confidence: 99%

Identification of the rpmF-plsX-fabH genes of Rhodobacter capsulatus

Carty

1994

FEMS Microbiology Letters

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The rpmF-plsX-fabH gene cluster of Rhodobacter capsulatus homologous to that of Escherichia coli was identified. rpmF encodes ribosomal protein L32, plsX plays an undefined role in membrane lipid synthesis, and fabH encodes beta-ketoacyl-acyl carrier protein synthase III. The R. capsulatus plsX gene complemented a defect in an E. coli strain with the plsX50 mutation. Overproduction of the fabH gene product of R. capsulatus in E. coli resulted in dramatically increased beta-ketoacyl-acyl carrier protein synthase III activity. These results indicate that plsX and fabH apparently function the same in R. capsulatus as in E. coli.

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Section: Discussionmentioning

confidence: 99%

Identification of the rpmF-plsX-fabH genes of Rhodobacter capsulatus

Carty

1994

FEMS Microbiology Letters

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“…Micro-organisms are able to adapt to the presence of toxic organic solvents, including aliphatic alcohols, using a whole cascade of adaptive mechanisms (for review : see [13 ]). Those leading to a modi¢cation of the membrane to keep it in the same £uidity condition have been described most intensively.…”

Section: Introductionmentioning

confidence: 99%

Effect of aliphatic alcohols on growth and degree of saturation of membrane lipids inAcinetobacter calcoaceticus

Kabelitz

Santos

Heipieper

2003

FEMS Microbiology Letters

128

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The adaptive responses of the bacterium Acinetobacter calcoaceticus to different aliphatic alcohols on the level of the membrane fatty acids were studied in detail. The toxicity of the aliphatic alcohols increased with an increasing hydrophobicity. As alcohols are known to increase the fluidity of the membrane they consequently should cause the same adaptive effect on membrane level. Yet, cells of A. calcoaceticus react completely different to the alcohols: in the presence of long-chained alcohols they increase their degree of saturation, while in the presence of short-chained alcohols they decrease the degree of saturation. So, there are no observable differences in the adaptive responses of bacteria with the so-called anaerobic pathway, like Escherichia coli and Pseudomonas putida, and the bacterium carrying the so-called aerobic pathway like A. calcoaceticus. These results strongly indicate a physico-chemical difference in the membrane effect of both the partitioning and localisation of the different alcohols into the membrane and the membrane adaptive responses of the bacteria to these effects.

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“…These cases are the knockout of fabA and fabB. The reason for this discrepancy is that both enzymes are essential in the production of unsaturated fatty acids [39]. Unsaturated fatty acids are also essential for processes not present in our model.…”

Section: Impact Of the Deficiencies In The Cell Wall-free Mutantmentioning

confidence: 89%

Theoretical study of lipid biosynthesis in wild‐type Escherichia coli and in a protoplast‐type L‐form using elementary flux mode analysis

et al. 2010

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In the present study, we investigated lipid biosynthesis in the bacterium Escherichia coli by mathematical modeling. In particular, we studied the interaction between the subsystems producing unsaturated and saturated fatty acids, phospholipids, lipid A, and cardiolipin. The present analysis was carried out both for the wild-type and for several in silico knockout mutants, using the concept of elementary flux modes. Our results confirm that, in the wild type, there are four main products: L1-phosphatidylethanolamine, lipid A, lipid A (cold-adapted), and cardiolipin. We found that each of these compounds is produced on several different routes, indicating a high redundancy of the system under study. By analysis of the elementary flux modes remaining after the knockout of genes of lipid biosynthesis, and comparison with publicly available data on single-gene knockouts in vivo, we were able to determine the metabolites essential for the survival of the cell. Furthermore, we analyzed a set of mutations that occur in a cell wallfree mutant of Escherichia coli W1655F+. We postulate that the mutant is not capable of producing both forms of lipid A, when the combination of mutations is considered to make a nonfunctional pathway. This is in contrast to gene essentiality data showing that lipid A synthesis is indispensable for the survival of the cell. The loss of the outer membrane in the cell wall-free mutant, however, shows that lipid A is dispensable as the main component of the outer surface structure in this particular E. coli strain.

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Regulation of fatty acid biosynthesis in Escherichia coli.

Cited by 378 publications

References 71 publications

Identification of the rpmF-plsX-fabH genes of Rhodobacter capsulatus

Identification of the rpmF-plsX-fabH genes of Rhodobacter capsulatus

Effect of aliphatic alcohols on growth and degree of saturation of membrane lipids inAcinetobacter calcoaceticus

Theoretical study of lipid biosynthesis in wild‐type Escherichia coli and in a protoplast‐type L‐form using elementary flux mode analysis

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