Regulation of Fibroblasts by Activated and Non-activated Immune Cells

Johnsson, Cecilia; Lorant, Tomas; Tufveson, Gunnar

doi:10.1016/j.healun.2005.06.010

Cited by 2 publications

(3 citation statements)

References 17 publications

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“…Cardiac fibroblasts appear to retain their in vivo properties for a long time after isolation and culturing. Thus, fibroblasts isolated from rejecting hearts show a higher proliferation rate, higher hyaluronan production and stronger CD44 expression than fibroblasts from nonrejecting heart grafts or from normal, nontransplanted hearts (8,9,16). These previous experiments were performed using pooled fibroblasts obtained from non-immunosuppressed allogeneically or syngeneically transplanted rats 5 days after transplantation.…”

Section: Fibroblasts and Allograft Rejectionmentioning

confidence: 99%

“…Different cell subtypes release different cytokines. Some of these cytokines, for example tumour necrosis factor-a and interferon-c produced by mononuclear cells, have stimulatory effects on fibroblasts (8,16). These enhancing effects appear to dominate over the inhibitory properties of the immunosuppressive drugs, which also reflects that mononuclear cells are the dominating cells during allograft rejection.…”

Section: Fibroblasts and Allograft Rejectionmentioning

confidence: 99%

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Graft morphology correlates with fibroblast activity in cardiac allograft rejection

Lorant

Emanuelsson

Quach

et al. 2011

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Several extracellular matrix substances, such as hyaluronan and fibronectin, may affect graft viability by their involvement in cell adhesion and in migration. These substances are produced locally in the tissue by fibroblasts. The aim of this study was to investigate the activation state of intragraft fibroblasts under various immunosuppressive treatments and to correlate these with morphological parameters. Syngeneic (n = 5) and allogeneic rat (n = 5-6/group) heterotopic heart transplantations were performed. Allogeneically transplanted animals were immunosuppressed with cyclosporine, mycophenolate mofetil or prednisolone. After 10 days, the transplanted hearts were removed for subsequent isolation of intragraft fibroblasts and for evaluation of graft morphology. The hyaluronan synthesis of graft fibroblasts correlated with the cellular infiltration (p < 0.05) and the interstitial oedema (p < 0.05) of the cardiac grafts. In general, proliferation rate and hyaluronan production were of the same magnitude in fibroblasts from allogeneic hearts under immunosuppression with cyclosporine, mycophenolate mofetil or prednisolone as in fibroblasts from syngeneic grafts. A pool of fibroblasts isolated from cardiac grafts of non-immunosuppressed, allogeneically transplanted rats (n = 4) showed considerably higher levels. We concluded that fibroblast activity correlates to the viability of the tissue rather than to the specific drug used for immunosuppression.

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Section: Fibroblasts and Allograft Rejectionmentioning

confidence: 99%

Section: Fibroblasts and Allograft Rejectionmentioning

confidence: 99%

Graft morphology correlates with fibroblast activity in cardiac allograft rejection

Lorant

Emanuelsson

Quach

et al. 2011

APMIS

Self Cite

View full text Add to dashboard Cite

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“…O uso de fibroblastos cardíacos pode ser considerado como uma estratégia interessante, já que estas células são de fácil obtenção, são transduzidas facilmente por vetores adenovirais e podem ser administradas em pacientes sem terapia imunossupressora (Johnsson et al,2004;Johnsson et al,2005).…”

Section: A Terapia Gênica Associada à Terapia Celularunclassified

Fibroblastos geneticamente modificados para estimular angiogênese e vasculogênese em miocárdio isquêmico

Gonçalves¹

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Sumário 3.3. Cultura primária de fibroblastos cardíacos e de músculos esqueléticos de ratos adultos 36 3.4. Cultura primária de mioblastos de músculos esqueléticos de ratos adultos 38 3.5. Infecção Celular 38 3.6. Fluorescência 39 3.7. Enzyme-linked immunoabsorbent assay (ELISA) 39 3.8. Ensaio de imunofluorescência para detecção das proteínas hVEGF e hIGF-1 em fibroblastos cardíacos modificados com os respectivos vetores virais 40 3.9. Ensaio Colorimétrico 42 3.10. Implante celular 42 3.11. Dosagem da atividade da enzima β-galactosidase 44 3.12. Lesão miocárdica por isquemia seguida de reperfusão-Experimento 1 ("Proof of Principle")-Teste de fisibilidade. 46 3.13. Lesão miocárdica por Ligadura permanente-Experimento 2-Teste de eficácia terapêutica. 47 3.14. Estudo morfológico por ecocardiograma 47 3.15. Estudo hemodinâmico da função cardíaca 49 3.16. Avaliação Morfométrica 50 3.17. Ensaio imunohistoquímico para detecção das proteínas hVEGF e hIGF-1 em tecidos de corações de rato 51 Sumário 3.18. Determinação de densidade capilar 53 3.19. Ensaio de imunofluorescência para detecção de vasculogênese em tecidos de corações de rato 53 3.20. Determinação da área de infarto e da área de colágeno 54 3.21. Extração de RNA 55 3.22. Transcrição Reversa (RT) 56 3.23. Reação de Polimerização em Cadeia (PCR) 57 3.24. Polímero de fibrina autólogo-Autologous fibrin polymer (fibrin sealant)-utilizado no Experimento 2-Teste de eficácia terapêutica.

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Regulation of Fibroblasts by Activated and Non-activated Immune Cells

Cited by 2 publications

References 17 publications

Graft morphology correlates with fibroblast activity in cardiac allograft rejection

Graft morphology correlates with fibroblast activity in cardiac allograft rejection

Fibroblastos geneticamente modificados para estimular angiogênese e vasculogênese em miocárdio isquêmico

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