Regulation of flavonol biosynthesis during anther and pistil development, and during pollen tube growth in <i>Solanum tuberosum</i>

Eldik, G.J. van; Reijnen, W. H.; Ruiter, R.K.; Herpen, M. M. A. van; Schrauwen, J. A. M.; Wullems, G. J.

doi:10.1046/j.1365-313x.1997.11010105.x

Cited by 50 publications

(30 citation statements)

References 41 publications

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“…The main characteristics of this stage are rapid growth of stamens and pistil and maturation of pollens. Several classes of genes were recognized as up-regulated genes in the hybrid panicles, and they are related to defense against phytopathogens (Paiva, 2000), mineral utilization, and flavonoid biosynthesis for flower coloring (Kazuma et al, 2003), pollen germination, and tube growth (van Eldik et al, 1997). Graminaceous plants secrete mugineic acid family phytosiderophores from their roots to solubilize Fe in the soil.…”

Section: Diverse Classes Of Up-regulated Genes Identified In the Hybrmentioning

confidence: 99%

Serial Analysis of Gene Expression Study of a Hybrid Rice Strain (LYP9) and Its Parental Cultivars

et al. 2005

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Using the serial analysis of gene expression technique, we surveyed transcriptomes of three major tissues (panicles, leaves, and roots) of a super-hybrid rice (Oryza sativa) strain, LYP9, in comparison to its parental cultivars, 93-11 (indica) and PA64s (japonica). We acquired 465,679 tags from the serial analysis of gene expression libraries, which were consolidated into 68,483 unique tags. Focusing our initial functional analyses on a subset of the data that are supported by full-length cDNAs and the tags (genes) differentially expressed in the hybrid at a significant level (P , 0.01), we identified 595 up-regulated (22 tags in panicles, 228 in leaves, and 345 in roots) and 25 down-regulated (seven tags in panicles, 15 in leaves, and three in roots) in LYP9. Most of the tag-identified and up-regulated genes were found related to enhancing carbon-and nitrogen-assimilation, including photosynthesis in leaves, nitrogen uptake in roots, and rapid growth in both roots and panicles. Among the downregulated genes in LYP9, there is an essential enzyme in photorespiration, alanine:glyoxylate aminotransferase 1. Our study adds a new set of data crucial for the understanding of molecular mechanisms of heterosis and gene regulation networks of the cultivated rice.

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Section: Diverse Classes Of Up-regulated Genes Identified In the Hybrmentioning

confidence: 99%

Serial Analysis of Gene Expression Study of a Hybrid Rice Strain (LYP9) and Its Parental Cultivars

et al. 2005

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“…Because CHS gene expression is regulated by various stimuli, including UV light (Tsukaya et al, 1991;Fuglevand et al, 1996;Ni et al, 1996;van Eldik et al, 1997;Hartmann et al, 1998;Kubasek et al, 1998), it was important to investigate if the constitutive expression of CHS in uvt1 was due to a defect in the UV-mediated regulation of the CHS gene. The data in Figure 9 (lane 4) show that CHS gene mRNA levels are significantly elevated after UVB exposure and, therefore, that the UVB-mediated regulation of CHS gene expression is still operating in uvt1.…”

Section: Chalcone Synthase (Chs) Mrna Levels In Uvt1 Are Constitutivementioning

confidence: 99%

An Arabidopsis Mutant Tolerant to Lethal Ultraviolet-B Levels Shows Constitutively Elevated Accumulation of Flavonoids and Other Phenolics

2001

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The isolation and characterization of mutants hypersensitive to ultraviolet (UV) radiation has been a powerful tool to learn about the mechanisms that protect plants against UV-induced damage. To increase our understanding of the various mechanisms of defense against UVB radiation, we searched for mutations that would increase the level of tolerance of Arabidopsis plants to UV radiation. We describe a single gene dominant mutation (uvt1) that leads to a remarkable tolerance to UVB radiation conditions that would kill wild-type plants. Pigment analyses show a constitutive increase in accumulation of UV-absorbing compounds in uvt1 that increases the capacity of the leaves to block UVB radiation and therefore is likely to be responsible for the elevated resistance of this mutant to UVB radiation. These increases in absorption in the UV region are due, at least in part, to increases in flavonoid and sinapate accumulation. Expression of chalcone synthase (CHS) mRNA was shown to be constitutively elevated in uvt1 plants, suggesting that the increases in absorption may be a consequence of changes in gene expression. Expression of CHS in uvt1 was shown to be still inducible by UV, indicating that the uvt1 lesion may not affect the UV-mediated regulation of CHS gene expression. Our data support an important role for UV screens in the overall protection of plants to UVB radiation. The uvt1 mutant could prove to be an important tool to elucidate further the exact role of UV-absorbing pigments in UV protection as well as the relative contribution of other mechanisms to the overall tolerance of plants to UV radiation.

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“…Transformation of petunia or tobacco with antisense FLS intensiWed red Xower pigmentation (Holton et al 1993). Further FLS cDNAs were isolated from Arabidopsis thaliana (Pelletier et al 1997;Wisman et al 1998), Eustoma grandiXorum, Solanum tuberosum (van Eldik et al 1997), Malus domestica and Matthiola incana (database submissions AF119095 and AF001391).…”

Section: Introductionmentioning

confidence: 99%

Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase

Stracke

Vos

Bartelniewoehner

et al. 2008

Planta

120

125

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Flavonol synthase (FLS) (EC-number 1.14.11.23), the enzyme that catalyses the conversion of Xavonols into dihydroXavonols, is part of the Xavonoid biosynthesis pathway. In Arabidopsis thaliana, this activity is thought to be encoded by several loci. In addition to the FLAVONOL SYNTHASE1 (FLS1) locus that has been conWrmed by enzyme activity assays, loci displaying similarity of the deduced amino acid sequences to FLS1 have been identiWed. We studied the putative A. thaliana FLS gene family using a combination of genetic and metabolite analysis approaches. Although several of the FLS gene family members are expressed, only FLS1 appeared to inXuence Xavonoid biosynthesis. Seedlings of an A. thaliana Xs1 null mutant (Xs1-2) show enhanced anthocyanin levels, drastic reduction in Xavonol glycoside content and concomitant accumulation of glycosylated forms of dihydroXavonols, the substrate of the FLS reaction. By using a leucoanthocyanidin dioxygenase (ldox) Xs1-2 double mutant, we present evidence that the remaining Xavonol glycosides found in the Xs1-2 mutant are synthesized in planta by the FLS-like side activity of the LDOX enzyme.

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Regulation of flavonol biosynthesis during anther and pistil development, and during pollen tube growth in Solanum tuberosum

Abstract: SummaryThe regulation of flavonol biosynthesis was studied in anthers and pistils of Solanum tuberosum.

Cited by 50 publications

References 41 publications

Serial Analysis of Gene Expression Study of a Hybrid Rice Strain (LYP9) and Its Parental Cultivars

Serial Analysis of Gene Expression Study of a Hybrid Rice Strain (LYP9) and Its Parental Cultivars

An Arabidopsis Mutant Tolerant to Lethal Ultraviolet-B Levels Shows Constitutively Elevated Accumulation of Flavonoids and Other Phenolics

Metabolomic and genetic analyses of flavonol synthesis in Arabidopsis thaliana support the in vivo involvement of leucoanthocyanidin dioxygenase

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