Regulation of gene expression by tobacco product preparations in cultured human dermal fibroblasts

Malpass, Gloria E.; Arimilli, Subhashini; Prasad, G.L.; Howlett, Allyn C.

doi:10.1016/j.taap.2014.06.001

Cited by 9 publications

(7 citation statements)

References 48 publications

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“…As illustrated in Table 3 , HDF expressed several proteins from the cholinergic pathway, such as acetylcholine receptors (nicotinic and muscarinic) [ 73 , 89 , 137 , 138 ] and acetylcholinesterase [ 92 ]. HDF respond to nicotinic and muscarinic agonists [ 90 , 139 ]. Moreover, muscarinic binding sites seem…”

Section: Resultsmentioning

confidence: 99%

Human Dermal Fibroblast: A Promising Cellular Model to Study Biological Mechanisms of Major Depression and Antidepressant Drug Response

Mesdom

Colle

Lebigot

et al. 2020

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Background: Human dermal fibroblasts (HDF) can be used as a cellular model relatively easily and without genetic engineering. Therefore, HDF represent an interesting tool to study several human diseases including psychiatric disorders. Despite major depressive disorder (MDD) being the second cause of disability in the world, the efficacy of antidepressant drug (AD) treatment is not sufficient and the underlying mechanisms of MDD and the mechanisms of action of AD are poorly understood. Objective: The aim of this review is to highlight the potential of HDF in the study of cellular mechanisms involved in MDD pathophysiology and in the action of AD response. Methods: The first part is a systematic review following PRISMA guidelines on the use of HDF in MDD research. The second part reports the mechanisms and molecules both present in HDF and relevant regarding MDD pathophysiology and AD mechanisms of action. Results: HDFs from MDD patients have been investigated in a relatively small number of works and most of them focused on the adrenergic pathway and metabolism-related gene expression as compared to HDF from healthy controls. The second part listed an important number of papers demonstrating the presence of many molecular processes in HDF, involved in MDD and AD mechanisms of action. Conclusion: The imbalance in the number of papers between the two parts highlights the great and still underused potential of HDF, which stands out as a very promising tool in our understanding of MDD and AD mechanisms of action.

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Section: Resultsmentioning

confidence: 99%

Human Dermal Fibroblast: A Promising Cellular Model to Study Biological Mechanisms of Major Depression and Antidepressant Drug Response

Mesdom

Colle

Lebigot

et al. 2020

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“…Several adverse cellular effects of combustible TPPs, in particular TPM, are reported to be associated with increased cytotoxic [27, 43–45], genotoxic [44], and proinflammatory effects [20]. However, the mechanism underlying TPP-induced [Ca 2+ ] i mobilization from internal and/or external Ca 2+ sources remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…We have previously assessed the effects of combustible and non-combustible tobacco product preparations (TPPs) on several functional endpoints in several cell types. For example, we have shown differential effects of combustible and non-combustible TPPs on DNA damage in oral cavity cells [19], regulation of gene expression in cultured human dermal fibroblasts [20], and immune cell function in NK and T cells [21]. Thus, a better understanding of the effects of the tobacco products will aid in developing rapid and functionally relevant tools for tobacco product evaluation.…”

Section: Introductionmentioning

confidence: 99%

Combustible Cigarette and Smokeless Tobacco Product Preparations Differentially Regulate Intracellular Calcium Mobilization in HL60 Cells

Arimilli¹,

Makena

Prasad

2019

Inflammation

Self Cite

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Changes in the level of intracellular calcium ([Ca 2+ ] i ) are central to leukocyte signaling and immune response. Although evidence suggests that cigarette smoking affects inflammatory response via an increase in intracellular calcium, it remains unclear if the use of smokeless tobacco ( e.g. , moist snuff) elicits a similar response. In this study, we evaluated the effects of tobacco product preparations (TPPs), including total particulate matter (TPM) from 3R4F reference cigarettes, smokeless tobacco extract (STE) from 2S3 reference moist snuff, and nicotine alone on Ca 2+ mobilization in HL60 cells. Treatment with TPM, but not STE or nicotine alone, significantly increased [Ca 2+ ] i in a concentration-dependent manner in HL60 cells. Moreover, TPM-induced [Ca 2+ ] i increase was not related to extracellular Ca 2+ and did not require the activation of the IP3 pathway nor involved the transient receptor potential (TRP) channels. Our findings indicate that, in cells having either intact or depleted endoplasmic reticulum (ER) Ca 2+ stores, TPM-mediated [Ca 2+ ] i increase involves cytosolic Ca 2+ pools other than thapsigargin-sensitive ER Ca 2+ stores. These results, for the first time, demonstrate that TPM triggers [Ca 2+ ] i increases, while significantly higher nicotine equivalent doses of STE or nicotine alone, did not affect [Ca 2+ ] i under the experimental conditions. In summary, our study suggests that in contrast with STE or nicotine preparations, TPM activates Ca 2+ signaling pathways in HL60 cells. The differential effect of combustible and non-combustible TPPs on Ca 2+ mobilization could be a useful in vitro endpoint for tobacco product evaluation.

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“…Malpass et al [33] analysed the differential gene expression of human microvascular endothelial cells (HMEC-1) induced by cisplatin using the gene chip dataset GSE62523 and found that both ATF3 and VCAM1 may be key genes associated with endothelial injury. High-density lipoprotein (HDL) exerts protects the vasculature by promoting the activation of transcription factors (such as ATF3), resulting in downregulation of the inflammatory response induced by Toll-like receptors (TLRs) [34].…”

Section: Endothelial Dysfunction Is Considered An Early Indicator Of mentioning

confidence: 99%

Research on the Correlation Between Activating Transcription Factor 3 Expression in the Human Coronary Artery and Atherosclerotic Plaque Stability

Peng

Xia

et al. 2021

Preprint

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Background: Activating transcription factor 3 (ATF3) is an early response gene that is activated in response to atherosclerotic stimulation and may be an important factor in inhibiting the progression of atherosclerosis. In this study, we directly measured the expression of ATF3 and inflammatory factors in human coronary atherosclerotic plaques to examine the relationship between ATF3 expression, inflammation and structural stability in human coronary atherosclerotic plaques. Methods: A total of 68 coronary artery specimens were collected from the autopsy group, including 36 cases of sudden death from coronary heart disease (SCD group) and 32 cases of acute death caused by mechanical injury with coronary atherosclerosis (CHD group). Twenty-two patients who had no coronary heart disease were collected as the control group (CON group). Haematoxylin-eosin staining was used to observe changes in arterial structure. Western blotting and immunohistochemistry were used to measure the expression and distribution of ATF3, Full list of author information is available at the end of the article-2-inflammatory factors and matrix metalloproteinase-9 (MMP-9) and vascular cell adhesion molecule 1 (VCAM1) in the coronary artery. The Pearson correlation coefficient was used to analyse the correlation between ATF3 protein expression and inflammatory factors and between ATF3 protein expression and structure-related indexes in the lesion group. Results: Compared with those in the control group, the intima and necrotic core in the coronary artery were thickened, the fibrous cap became thin and the degree of vascular stenosis was increased in the lesion group, while the intima and necrotic core became thicker and the fibrous cap became thinner in the SCD group than in the CHD group (P < 0.05). There was no or low expression of ATF3, inflammatory factors, VCAM1 and MMP-9 in the control group, and the expression of inflammatory factors, VCAM1 and MMP-9 in the SCD group was higher than that in CHD group, while the expression of ATF3 in the SCD group was significantly lower than that in CHD group (P < 0.05). In the lesion group, the expression of ATF3 was negatively correlated with intimal and necrotic focus thickness, positively correlated with fibrous cap thickness (P < 0.01), and negatively correlated with inflammatory factors, VCAM1 and MMP-9 (P < 0.01). Conclusions: The expression of ATF3 may be related to the progression and stability of atherosclerotic plaques, and may affect the structural stability of atherosclerotic plaques by regulating the inflammatory response, thus participating in the regulation of atherosclerotic progression.

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Regulation of gene expression by tobacco product preparations in cultured human dermal fibroblasts

Cited by 9 publications

References 48 publications

Human Dermal Fibroblast: A Promising Cellular Model to Study Biological Mechanisms of Major Depression and Antidepressant Drug Response

Human Dermal Fibroblast: A Promising Cellular Model to Study Biological Mechanisms of Major Depression and Antidepressant Drug Response

Combustible Cigarette and Smokeless Tobacco Product Preparations Differentially Regulate Intracellular Calcium Mobilization in HL60 Cells

Research on the Correlation Between Activating Transcription Factor 3 Expression in the Human Coronary Artery and Atherosclerotic Plaque Stability

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