Regulation of Glucose Phosphate Isomerase by the 3′UTR-Specific miRNAs miR-302b and miR-17-5p in Chicken Primordial Germ Cells1

Rengaraj, Deivendran; Park, Tae Sub; Lee, Sang In; Lee, Bo Ram; Han, Beom Ku; Song, Gwonhwa; Han, Jae Yong

doi:10.1095/biolreprod.112.105692

Cited by 27 publications

(16 citation statements)

References 38 publications

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“…miR-140-5p downregulated the expression of TGF-βII receptors, while miR-23b and let-7 g upregulated TGF-βII receptor activity (Yang, Fang, Chang, & Yang, 2013). The clinical data confirmed that miR-17-5p and miR-20 could also regulate the TGF-βII receptor to influence the HTS processes (Dews et al, 2010;Rengaraj et al, 2013). Alao, were also considered to influence TGF-β1 to regulate collagen synthesis (Ferrante et al, 2015;Zhou et al, 2017).…”

Section: Mirna Regulation Of Specific Target Genes or Proteinmentioning

confidence: 68%

Molecular mechanism of myofibroblast formation and strategies for clinical drugs treatments in hypertrophic scars

Zhu

Hou

et al. 2019

Journal Cellular Physiology

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Hypertrophic scars (HTS) commonly occurred after burn and trauma. It was characterized by the excessive deposition of extracellular matrix with the inadequate remodeling, which could result in severe physiological and psychological problems.However, the effective available prevention and treatment measures were still limited. The main pathological feature of HTS was the excessive formation of myofibroblasts, and they persist in the repaired tissue. To better understand the mechanics of this process, this review focused on the characteristics and formation of myofibroblasts, the main effector cells in HTS. We summarized the present theories and opinions on myofibroblasts formation from the perspective of related signaling pathways and epigenetic regulation, such as DNA methylation, miRNA/lncRNA/ ceRNA action, histone modification, and so forth for a better understanding on the development of HTS. This information might assist in developing effective experimental and clinical treatment strategies. Additionally, we also summarized currently known clinical strategies for HTS treatment, including traditional drugs, molecular medicine, stem cells, and exosomes. K E Y W O R D S epigenetic mechanism, extracellular matrix, hypertrophic scars, myofibroblast, stem cells

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Section: Mirna Regulation Of Specific Target Genes or Proteinmentioning

confidence: 68%

Molecular mechanism of myofibroblast formation and strategies for clinical drugs treatments in hypertrophic scars

Zhu

Hou

et al. 2019

Journal Cellular Physiology

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“…Several studies have reported that the overexpression of miRNAs downregulates their target genes, while downregulation of miRNAs upregulates their target genes, owing to their post-transcriptional gene regulatory functions [7]. This hypothesis is somewhat supported in the case of gga-miR-9-5p, gga-miR-20b-5p, gga-miR-196-5p, and gga-let-7d and the expression of their target genes MAP3K3 , MAP3K2 , AQP4 , and IGF2BP3 , respectively in the IML of NE-induced M5.1 chickens.…”

Section: Resultsmentioning

confidence: 99%

“…The expression and/or regulation of miRNAs are crucial for various biological activities including cell proliferation and differentiation, embryo development, disease progression, and cellular metabolisms. Several studies have reported that miRNAs preferentially bind to the 3′-untranslated region (3′UTR) during post-transcriptional gene regulation [6,7]. miRNAs regulate genes in both normal and disease conditions; however, the expression of miRNAs itself altered in most cancer and other diseases.…”

Section: Introductionmentioning

confidence: 99%

Distribution and differential expression of microRNAs in the intestinal mucosal layer of necrotic enteritis induced Fayoumi chickens

Rengaraj

Truong

Ban

et al. 2017

Asian-Australas J Anim Sci

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ObjectiveDespite an increasing number of investigations into the pathophysiology of necrotic enteritis (NE) disease, etiology of NE-associated diseases, and gene expression profiling of NE-affected tissues, the microRNA (miRNA) profiles of NE-affected poultry have been poorly studied. The aim of this study was to induce NE disease in the genetically disparate Fayoumi chicken lines, and to perform non-coding RNA sequencing in the intestinal mucosal layer.MethodsNE disease was induced in the Fayoumi chicken lines (M5.1 and M15.2), and non-coding RNA sequencing was performed in the intestinal mucosal layer of both NE-affected and uninfected chickens to examine the differential expression of miRNAs. Next, quantitative real-time polymerase chain reaction (real-time qPCR) was performed to further examine four miRNAs that showed the highest fold differences. Finally, bioinformatics analyses were performed to examine the four miRNAs target genes involvement in the signaling pathways, and to examine their interaction.ResultsAccording to non-coding RNA sequencing, total 50 upregulated miRNAs and 26 downregulated miRNAs were detected in the NE-induced M5.1 chickens. While 32 upregulated miRNAs and 11 downregulated miRNAs were detected in the NE-induced M15.2 chickens. Results of real-time qPCR analysis on the four miRNAs (gga-miR-9-5p, gga-miR-20b-5p, gga-miR-196-5p, and gga-let-7d) were mostly correlated with the results of RNAseq. Overall, gga-miR-20b-5p was significantly downregulated in the NE-induced M5.1 chickens and this was associated with the upregulation of its top-ranking target gene, mitogen-activated protein kinase, kinase 2. Further bioinformatics analyses revealed that 45 of the gene targets of gga-miR-20b-5p were involved in signal transduction and immune system-related pathways, and 35 of these targets were predicted to interact with each other.ConclusionOur study is a novel report of miRNA expression in Fayoumi chickens, and could be very useful in understanding the role of differentially expressed miRNAs in a NE disease model.

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“…However, what is even more interesting is that it has been also shown that these miRNAs regulate brain-derived neurotrophic factor (BDNF) [30][31][32]. Other miRNAs differentiating the SWR/J control from the WT control group of mice, such as miR-20 and miR-106, are associated with the regulation of glucose metabolism [33], whilst miR-93 and miR-106 might affect liver diseases [34]. Additionally, miR-106 has been linked with regulation of the MAPK signaling pathway during oxidative stress [35].…”

Section: Discussionmentioning

confidence: 99%

Serum Level of miR-1 and miR-155 as Potential Biomarkers of Stress-Resilience of NET-KO and SWR/J Mice

Solich

Kuśmider

Faron-Górecka

et al. 2020

Cells

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In the present study, we used three strains of mice with various susceptibility to stress: mice with knock-out of the gene encoding norepinephrine transporter (NET-KO), which are well characterized as displaying a stress-resistant phenotype, as well as two strains of mice displaying two different stress-coping strategies, i.e., C57BL/6J (WT in the present study) and SWR/J. The procedure of restraint stress (RS, 4 h) was applied, and the following behavioral experiments (the forced swim test and sucrose preference test) indicated that NET-KO and SWR/J mice were less sensitive to RS than WT mice. Then, we aimed to find the miRNAs which changed in similar ways in the serum of NET-KO and SWR/J mice subjected to RS, being at the same time different from the miRNAs found in the serum of WT mice. Using Custom TaqMan Array MicroRNA Cards, with primers for majority of miRNAs expressed in the serum (based on a preliminary experiment using the TaqMan Array Rodent MicroRNA A + B Cards Set v3.0, Thermo Fisher Scientific, Waltham, MA, USA) allowed the identification of 21 such miRNAs. Our further analysis focused on miR-1 and miR-155 and their targets-these two miRNAs are involved in the regulation of BDNF expression and can be regarded as biomarkers of stress-resilience.Cells 2020, 9, 917 2 of 17 are regarded as endogenous "hubs" (as Issler and Chen have put it) for the fine tuning of target gene expression or an "expression switch", and can provide deeper insight into complex biological processes underlying stress response.In the present study, we used three strains of mice differentially reacting to stress: mice with knock-out of the gene encoding norepinephrine transporter (NET-KO), which are well characterized as displaying a stress-resistant phenotype [7-9], as well as two other strains of mice displaying different stress-coping strategies, i.e., C57BL/6J (WT in the present study) and swiss SWR/J, described extensively by Szklarczyk and co-workers [10]. The different reaction to stress of these strains of mice has been confirmed by measuring corticosterone level. The NET-KO mice display a slower increase in corticosterone level after stress compared with WT animals, while corticosterone level was not changed at 2 h after restraint stress in the blood of SWR/J mice [8,10]. We applied the procedure of restraint stress (RS), which has been shown to induce an uncontrollable aversive situation that produces both physical and psychological consequences leading to neuronal and behavioral alterations [11]. The aim of following molecular studies was to find biomarkers for different behavioral responses to RS among miRNAs expressed in the serum of three genotypes under study. This goal was achieved, as we were able to identify a group of miRNAs differentiating the stress response of NET-KO and SWR/J mice from WT animals. The most interesting were the alterations in the miR-1 and miR-155 levels, which are involved in regulation of BDNF expression, which in turn affects important biochemical processes. Materials and Methods AnimalsH...

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Regulation of Glucose Phosphate Isomerase by the 3′UTR-Specific miRNAs miR-302b and miR-17-5p in Chicken Primordial Germ Cells1

Cited by 27 publications

References 38 publications

Molecular mechanism of myofibroblast formation and strategies for clinical drugs treatments in hypertrophic scars

Molecular mechanism of myofibroblast formation and strategies for clinical drugs treatments in hypertrophic scars

Distribution and differential expression of microRNAs in the intestinal mucosal layer of necrotic enteritis induced Fayoumi chickens

Serum Level of miR-1 and miR-155 as Potential Biomarkers of Stress-Resilience of NET-KO and SWR/J Mice

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