Regulation of glycolysis in biotin-deficient rat liver

Biotin Administration Improves the Impaired Glucose Tolerance of Streptozotocin-Induced Diabetic Wistar Rats.

“…The effect of biotin administration on the glucose tolerance It has been known for many years that biotin deficiency results in an impair ment of glucose utilization (1)(2)(3)(4)(5). In our previous study , insulin secretion in response to an oral glucose load in biotin-deficient male Wistar rats was approxi mately one-sixth less, in concentration terms, than that of pair-fed control rats .…”

Temporal development of genetic and metabolic effects of biotin deprivation. A search for the optimum time to study a vitamin deficiency

“…Biotin deficiency can be produced experimentally by adding avidin to the animals' diet, and its full blown effects are usually observed at 6-8 weeks of feeding the deficiency producing diet [2,5,6,[8][9][10][11][12][13][14]. This vitamin deprivation bears several similarities to multiple carboxylase deficiency, a group of inherited metabolic disorders caused by mutations in the genes for intestinal biotin transport [15], holocarboxylase synthetase [16], and biotinidase [17], in that in all of these conditions the activities of the different carboxylases are greatly reduced.…”

Holocarboxylase synthetase is an obligate participant in biotin-mediated regulation of its own expression and of biotin-dependent carboxylases mRNA levels in human cells

“…In these studies, biotin seems to augment the enzymatic activity of glucokinase and the transcription of its gene in rats in vivo, pancreatic ␤-cells and rat hepatocytes in culture (21-23). Similarly, mRNA levels for 6-phosphofructokinase are increased after administration of the vitamin to biotin-starved rats (24,25). In human hepatocyte culture, biotin seems to be required to promote translation of the asialoglycoprotein receptor (ASGR) mRNA (26).…”