Regulation of human osteoblast integrin expression by orthopedic implant materials

Sinha, Raj; Tuan, Rocky S.

doi:10.1016/8756-3282(96)00044-0

Cited by 186 publications

(139 citation statements)

References 44 publications

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“…Moreover, the integrin a 5 Fwhich is a major adhesion receptor of osteoblasts interacting with FN [26,27]Fwas not detected in cell cultured on polished or rough Ti-6A1-4V at 12 h while it was detected for cell cultured on polystyrene [28]. Despite results of the study by Sinha and Tuan [28] were obtained at 12 h while present FN results were obtained at week 1, they could support the hypothesis that proteins other than FN were involved in the osteoblast adhesion to Ti alloy. The production of FN observed after two weeks could then probably be due to cell-cell contact e.g.…”

Section: Discussionmentioning

confidence: 95%

Effect of different Ti–6Al–4V surface treatments on osteoblasts behaviour

Ku¹,

et al. 2002

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The purpose of the present work was to examine the effect of different Ti-6Al-4V surface treatments on osteoblasts behaviour. Previous work in this laboratory has demonstrated that an ageing treatment reduces metal ion release from this alloy compared to standard passivation procedures. In this study, human osteosarcoma MG-63 were used in short-term in vitro tests to assay for cell viability and cell proliferation at 12, 24 and 72 h while SaOS-2 were used in long-term in vitro tests to assay for osteonectin, osteopontin, osteocalcin gene expression, total protein amount (TP), alkaline phosphatase activity (ALP) and fibronectin production (FN) for 1-4 weeks. Epifluorescence microscopy was used to observe SaOS-2 cell morphology. After 24 h, there was no difference in MG-63 cell viability/proliferation or in SaOS-2 cell morphology between the different surface treatments. For the longterm tests, the aged Ti-6Al-4V induced significantly higher cell proliferation than the control Ti-6Al-4V at 72 h. At week 1, no difference in the osteonectin, osteopontin, and osteocalcin gene expression was found between samples. The peak of ALP activity appeared earlier at week 2 for the control surface compared with the passivated and aged surfaces. The early increase in ALP activity for the control sample could be a compensatory effect of decreased osteoblasts proliferation. There was no difference in the expression of FN for the different surface treatments. Our present results showed that the different surface treatments, which induced different metal ion release kinetics and surface properties, influenced the cell proliferation and ALP activity of osteoblast cells. Aluminium ions release kinetics as well as presence of vanadium ions may play a major role in influencing the osteoblasts behaviour in the present study. r

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Section: Discussionmentioning

confidence: 95%

Effect of different Ti–6Al–4V surface treatments on osteoblasts behaviour

Ku¹,

et al. 2002

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“…29 Increasing surface roughness has been shown to increase osteoblast adhesion and differentiation 30,31 and this response appears to be mediated by specific integrins on cells. 32,33 Therefore, it is possible that the specific nano/microtexture of Ta can promote cell adhesion by upregulation of specific integrins. SEM micrographs also demonstrated a unique three-dimensional structure of Ta with a prominent rough surface.…”

Section: Tantalum Stimulates Proliferationmentioning

confidence: 99%

“…The Institutional Review Board of the University of Connecticut Health Center determined that this study did not constitute human study research. Female patients in good health under the age of 45 were designated as younger (22,28,29,33,39, and 44), while those older than 60 years of age were designated as older (61, 63, 67, 68, 71, 73, and 75 years old). After removal of soft tissue, bone fragments were minced and cultured in proliferation medium of DMEM/F-12 with 10% fetal bovine serum (FBS) and 1% penicillin (100 U/ ml) and streptomycin sulfate (100 mg/ml) (GibcoBRL, Grand Island, NY).…”

Section: Cell Culturementioning

confidence: 99%

Porous tantalum stimulates the proliferation and osteogenesis of osteoblasts from elderly female patients

Sagomonyants

Hakim-Zargar

Jhaveri

et al. 2010

Journal Orthopaedic Research

100

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Porous tantalum (Ta) implants have been successful in various orthopedic procedures for patients with compromised boneforming abilities. Previous studies demonstrated that human osteoblast (HOB) cultures from older female patients produced less bone on implant materials in vitro compared to HOBs from age-matched male and younger female patients. In this study, the responses of HOBs from younger (<45) and older (>60 years old) female patients were compared on Ta, titanium fiber mesh (TFM) and tissue culture plastic. Adhesion, proliferation, and mineralization were greater in cells from younger patients than from older patients. Cell adhesion was slightly higher on Ta than TFM or plastic. However, Ta highly stimulated cell proliferation with a 4-and 6-fold increase compared to TFM for cells from younger and older patients, respectively, and 12-and 16-fold increase in proliferation compared to cells on plastic ( p = 0.001). At 3 weeks, mineralization was significantly higher on Ta compared to TFM for HOBs from older patients ( p = 0.05). Expression levels of bone matrix markers demonstrated differences dependent on age and substrate. Scanning electron micrographs revealed HOBs covering the surfaces and entering the pores of both Ta and TFM. In conclusion, tantalum greatly stimulates cell proliferation, and improves the ability of HOBs from older patients to form bone. ß

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“…Specifically, we have compared the performance of two basal media, Dulbecco's modified Eagle's medium (DMEM), widely used for the culture of various cell types including bone marrow-derived MPCs [14,18,19], and DMEM with Kaighn's modification of Ham's F12 (F12K) supplemented with ascorbic acid and L-glutamine, which has been routinely used in studies involving cells derived from trabecular bone [9,20,21].…”

Section: ® Original Articlementioning

confidence: 99%

“…The in vitro culture of human trabecular bone-derived cells has served as a useful system for the investigation of the biology of osteoblasts and has also provided insights into the interactions between bone and biomaterial for orthopedic and dental implants, their response to different growth factors and hormones, cell-matrix interactions, and osteoblast differentiation and maturation [1][2][3][4][5][6][7][8][9]. Our recent discovery of the multilineage mesenchymal differentiation potential of primary cell cultures derived from collagenase-treated human…”

Section: Introductionmentioning

confidence: 99%

Characterization of Multipotential Mesenchymal Progenitor Cells Derived from Human Trabecular Bone

et al. 2003

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ABSTRACT-cell population resident within collagenase-treated, culture-processed bone fragments, which upon migration established a homogeneous population of MPCs. Additionally, we have introduced a system of culturing these MPCs that best supports and maintains their optimal differentiation potential during long-term culture expansion. When cultured as described, the trabecular bone-derived cells display stem cell-like capabilities, characterized by a stable undifferentiated phenotype as well as the ability to proliferate extensively while retaining the potential to differentiate along the osteoblastic, adipocytic, and chondrocytic lineages, even when maintained in long-term in vitro culture.

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Regulation of human osteoblast integrin expression by orthopedic implant materials

Cited by 186 publications

References 44 publications

Effect of different Ti–6Al–4V surface treatments on osteoblasts behaviour

Effect of different Ti–6Al–4V surface treatments on osteoblasts behaviour

Porous tantalum stimulates the proliferation and osteogenesis of osteoblasts from elderly female patients

Characterization of Multipotential Mesenchymal Progenitor Cells Derived from Human Trabecular Bone

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