Regulation of IL-1β-Mediated MUC2 Gene in NCI-H292 Human Airway Epithelial Cells

Kim, Yong Dae; Kwon, Eun Jin; Kwon, Taeg Kyu; Baek, Suk‐Hwan; Youn, Song， Si; Suh, Jang Su

doi:10.1006/bbrc.2000.3107

Cited by 43 publications

(41 citation statements)

References 12 publications

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“…These results of mucin secretion were consistent with gene expression data. These data were similar to those of our previous study (6).…”

Section: Induction Of the Muc2 Gene Expression And Mucin Secretion Bysupporting

confidence: 93%

“…Our recent study (6) demonstrated that IL-1 stimulates the expression of MUC2 gene and mucin secretion by transcriptional regulation. However, we could not delineate the specific signal pathway involved in the mediation of mucin secretion in that study.…”

Section: Introductionmentioning

confidence: 94%

“…The method used to detect and quantify the MUC2 mRNA level employed a modified technique of RT-PCR and has been described previously in detail (6). Briefly, total RNA was reverse transcribed into cDNA using random hexanucleotide primers and MULV reverse transcriptase (Perkin-Elmer, Morrisville, NC).…”

Section: Rt-pcr Analysis Of the Muc2 Genementioning

confidence: 99%

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Interleukin-1 beta Induces MUC2 Gene Expression and Mucin Secretion via Activation of PKC-MEK/ERK,and PI3K in Human Airway Epithelial Cells

et al. 2002

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Interleukin 1beta(IL-1beta), a proinflammatory cytokine, is related with inflammatory diseases and it up-regulates MUC2 gene expression and mucin secretion. This study was designed to investigate the signal transduction pathway of the IL-1beta-mediated MUC2 gene expression and mucin secretion in human airway epithelial cells. In cultured human airway NCI-H292 epithelial cells, the steady state of the mRNA level of MUC2 gene expression and mucin secretion induced by IL-1beta were determined by reverse transcriptase-polymerase chain reaction (RT-PCR), enzyme immunoassay, and immunoblot analysis. To observe the signal pathway of the IL-1beta-mediated MUC2 gene expression and mucin secretion, we used several specific inhibitors. PD98059 (MEK/ERK inhibitor) suppressed IL-1beta-mediated MUC2 gene expression and mucin secretion, while SB203580 (p38 inhibitor) did not. Ro31-8220 (PKC inhibitor) inhibited IL-1beta-mediated MUC2 gene expression and mucin secretion. It inhibited ERK phosphorylation, but did not inhibit p38 phosphorylation. LY294002 (PI3K inhibitor) also suppressed MUC2 expression, but did not inhibit any MAPKs phosphorylation. These results suggest that the IL-1beta-mediated MUC2 gene expression and mucin secretion in NCI-H292 cells are regulated through activation of the PKC-MEK/ERK pathway, and that PI3K is also involved in the IL-1beta-mediated MUC2 gene expression and mucin secretion.

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“…These results of mucin secretion were consistent with gene expression data. These data were similar to those of our previous study (6).…”

Section: Induction Of the Muc2 Gene Expression And Mucin Secretion Bysupporting

confidence: 93%

Section: Introductionmentioning

confidence: 94%

See 1 more Smart Citation

Interleukin-1 beta Induces MUC2 Gene Expression and Mucin Secretion via Activation of PKC-MEK/ERK,and PI3K in Human Airway Epithelial Cells

et al. 2002

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“…In the human colon carcinoma cell line, paracrine stimulation by a soluble factor from human colon connective tissue was involved in inducing the expression of the MUC1 mucin in vitro (Irimura et al, 1990). Cytokine-like tumour necrosis factor-a, interleukins, and EGF have been shown to be involved in the regulation of mucin gene expression Longphre et al, 1999;Takeyama et al, 1999;Kim et al, 2000;Smirnova et al, 2000). One of the cytokines (i.e.…”

Section: Discussionmentioning

confidence: 99%

MUC4 mucin expression in human pancreatic tumours is affected by organ environment: the possible role of TGFβ2

et al. 2004

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MUC4 is highly expressed in human pancreatic tumours and pancreatic tumour cell lines, but is minimally or not expressed in normal pancreas or chronic pancreatitis. Here, we investigated the aberrant regulation of MUC4 expression in vivo using clonal human pancreatic tumour cells (CD18/HPAF) grown either orthotopically in the pancreas (OT) or ectopically in subcutaneous tissue (SC) in the nude mice. Histological examination of the OT and SC tumours showed moderately differentiated and anaplastic morphology, respectively. The OT tumour cells showed metastases to distant lymph nodes and faster tumour growth (Po0.01) compared to the SC tumours. The MUC4 transcripts in OT tumours were very high compared to the undetectable levels in SC tumours. The SC tumour cells regained their ability to express MUC4 transcripts after in vitro culture. Immunohistochemical analysis using MUC4-specific polyclonal antiserum confirmed the results obtained by Northern blot analysis. Interestingly, the OT tumours showed expression of TGFb2 compared to no expression in SC, suggesting a possible link between MUC4 and TGFb2. The MUC4 expression, morphology, and metastasis of human pancreatic tumour cells are regulated by a local host microenvironment. TGFb2 may serve as an interim regulator of this function.

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“…This suggests that TNF-α induces MUC2 by a transcriptional mechanism but that the TNF-responsive element may be beyond the 2.8 kb of the transcriptional start site. IL-1β also stimulates MUC2 transcription in NCI-H292 cells (47). In one report, a retinoic acid receptor (RAR)-α antagonist could inhibit the induction of MUC2 by IL-1β and TNF-α, suggesting that IL-1β and TNF-α act partly through RAR pathways (48).…”

Section: Muc2 Expression Regulated By Inflammatory Cytokinesmentioning

confidence: 99%

Regulation of Airway Mucin Gene Expression

Thai

Loukoianov

Wachi

et al. 2008

Annu. Rev. Physiol.

193

223

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Mucins are important components that exert a variety of functions in cell-cell interaction, epidermal growth factor receptor signaling, and airways protection. In the conducting airways of the lungs, mucins are the major contributor to the viscoelastic property of mucous secretion, which is the major barrier to trapping inhaled microbial organism, particulates, and oxidative pollutants. The homeostasis of mucin production is an important feature in conducting airways for the maintenance of mucociliary function. Aberrant mucin secretion and accumulation in airway lumen are clinical hallmarks associated with various lung diseases, such as asthma, chronic obstructive pulmonary disease, cystic fibrosis, emphysema, and lung cancer. Among 20 known mucin genes identified, 11 of them have been verified at either the mRNA and/or protein level in airways. The regulation of mucin genes is complicated, as are the mediators and signaling pathways. This review summarizes the current view on the mediators, the signaling pathways, and the transcriptional units that are involved in the regulation of airway mucin gene expression. In addition, we also point out essential features of epigenetic mechanisms for the regulation of these genes.

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Regulation of IL-1β-Mediated MUC2 Gene in NCI-H292 Human Airway Epithelial Cells

Cited by 43 publications

References 12 publications

Interleukin-1 beta Induces MUC2 Gene Expression and Mucin Secretion via Activation of PKC-MEK/ERK,and PI3K in Human Airway Epithelial Cells

Interleukin-1 beta Induces MUC2 Gene Expression and Mucin Secretion via Activation of PKC-MEK/ERK,and PI3K in Human Airway Epithelial Cells

MUC4 mucin expression in human pancreatic tumours is affected by organ environment: the possible role of TGFβ2

Regulation of Airway Mucin Gene Expression

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