Regulation of Immunoreactive Activin A Secretion from Cultured Rat Anterior Pituitary Cells.

Liu, Zhonghui; Shintani, Yoshihiro; Wakatsuki, M; Sakamoto, Yuki; Harada, Kyoko; Zhang, Chenyu; Saito, Shiro

doi:10.1507/endocrj.43.39

Cited by 22 publications

(11 citation statements)

References 30 publications

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“…Although the mechanism remains unclear, this stimulatory effect of LHRH on ir-follistatin release may be mediated by pituitary activin. We also found that ir-activin A seretion is significantly enhanced by LHRH in cultured rat anterior pituitary cells [21]. Furthermore, DePaolo et al demonstrated that pituitary follistatin expression is enhanced after castration by the mediation of pituitary activin [22].…”

Section: Molecular Heterogeneity Of Follistatin In the Culture Mediumsupporting

confidence: 67%

“…There has been no direct evidence for the presence of FSH receptor in the pituitary, but we recently demonstrated that pituitary activin A secretion is suppressed by the addition of FSH [21], implying the possiblity that FSH may also exert a form of direct regulation in the pituitary, but further investigation is necessary to prove this hypothesis.…”

Section: Molecular Heterogeneity Of Follistatin In the Culture Mediummentioning

confidence: 90%

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Effects of LHRH, FSH and Activin A on Follistatin Secretion from Cultured Rat Anterior Pituitary Cells.

et al. 1996

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Abstract.We demonstrated the release of follistatin, an activin-binding protein, from cultured rat anterior pituitary cells by measuring immunoreactive (ir-) follistatin in a specific immunoradiometric assay. Ir-follistatin release gradually increased in cultures over 1-18 days and reached its maximal level at 12-15 days of incubation.The basal ir-follistatin levels in the culture media increased about 3-(P< 0.01) and 5-fold (P< 0.001) in 2 and 10% fetal calf serum for 6 days, respectively.LHRH and activin A caused an approximately 2.0-(P< 0.05) and 1.8-fold (P< 0.05) rise in ir-follistatin release, respectively, in contrast to the lack of significant FSH effects. The culture medium condensed on sulfate-cellulofine gel was resolved by polyacrylamide gel electrophoresis and blotted with anti-follistatin polyclonal antibody, resulting in at least three protein bands ranging from 35 to 50 kDa under non-reducing conditions. These results indicated that follistatin is produced in anterior pituitary cells and that its secretion is regulated at least in part by LHRH and activin, implying an autocrine/paracrine role of activin and follistatin in the pituitary.

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Section: Molecular Heterogeneity Of Follistatin In the Culture Mediumsupporting

confidence: 67%

Section: Molecular Heterogeneity Of Follistatin In the Culture Mediummentioning

confidence: 90%

Effects of LHRH, FSH and Activin A on Follistatin Secretion from Cultured Rat Anterior Pituitary Cells.

et al. 1996

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“…This does not exclude the fact that GnRH plays a role in stimulating the high FSH concentrations due to the absence of negative feedback in barrows. From other species it is known that GnRH and activin interact (Liu et al, 1996;Shupnik and Weck, 1998;Gregory et al, 2005) so that high GnRH concentrations might be paralleled by elevated activin. Because activin in turn inhibits the synthesis and release of GH (Childs and Unabia, 1997), these effects might also explain lower GH levels in surgical castrates.…”

Section: Discussionmentioning

confidence: 99%

Effects of immunization against GnRH on gonadotropins, the GH-IGF-I-axis and metabolic parameters in barrows

Bauer

Lacorn

Danowski

et al. 2008

Animal

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Surgically castrated male piglets (barrows) reveal an increase in LH and a decrease in GH compared to untreated boars. Boars that were castrated by immunization against gonadotropin releasing hormone (GnRH) have decreased LH but maintain GH. The difference in GH levels between barrows and immunological castrated boars cannot be explained by testicular steroids because they are low in surgical and immunocastrated boars as well. Therefore, differences in GH concentrations might be due to an interaction between GnRH and growth hormone releasing hormone (GRH) in the hypothalamus or the pituitary. This hypothesis was tested with twelve male piglets that had been castrated within 1 week postnatally and fitted with indwelling cephalic vein catheters at 17 weeks of age. They were split into a control group and an immunized group (each n 5 6). Vaccination with Improvac R was performed at 18 and 22 weeks of age. Specific radioimmunoassays were used for hormone determinations (GH, LH, FSH, testosterone and IGF-I). Additionally, metabolic responses were evaluated by measuring analytical parameters that characterize protein synthesis and breakdown, and body fat content. The second vaccination led to a rapid decrease of LH below the limit of detection whereas FSH decreased more slowly, over a period of 5 weeks, from 2.2 to 0.5 ng/ml. This level of FSH, which corresponds to boar-specific concentrations, was maintained thereafter. GH decreased with increasing age but was not influenced by vaccination and remained at a low concentration typical for barrows. Similarly, IGF-I was not altered by vaccination. Consequently, metabolic status was not changed by immunization. It is concluded that the difference in GH levels between surgical and immunocastrated boars is not explained by an interaction between GnRH and GRH.

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“…Originally isolated from the gonads, activin has been detected in the rat anterior pituitary (Meunier et al 1988, Roberts et al 1989, Bilezikjian et al 1993, Halvorson et al 1994, Liu et al 1996, where it may play an autocrine/ paracrine role in the regulation of anterior pituitary hormones (Corrigan et al 1991, Besecke et al 1996. Recent studies have demonstrated that it has specific stimulatory effects on follicle-stimulating hormone (FSH) gene expression in the pituitary and FSH secretion in culture but has no effect with regard to luteinizing hormone (LH) (Vale et al 1986, Ling et al 1986, Kitaoka et al 1987, Ying 1988.…”

Section: Introductionmentioning

confidence: 99%

Effects of activin on hormone secretion by single female rat pituitary cells: analysis by cell immunoblot assay

Miyamoto

Irahara²,

Ushigoe³

et al. 1999

Journal of Endocrinology

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We investigated the effect of activin A on secretion of LH, FSH, and prolactin (PRL) by female cultured rat pituitary cells at the single-cell level by means of the cell immunoblot assay. Anterior pituitary cells from 8-week-old female rats were preincubated with or without activin A for 24 h, after which they were monodispersed and immediately used for cell immunoblot assay.The percentages of LH-, FSH-and PRLimmunoreactive cell blots detected were 5·5, 5·3 and 43·1%, respectively, of all pituitary cells applied to the transfer membrane. The percentage of LH-secreting cells and mean LH secretion per cell did not change after treatment with activin. In contrast, activin significantly increased the percentage of FSH-secreting cells and mean FSH secretion per cell to 136·0 and 114·5% respectively. In addition, activin significantly decreased the percentage of PRL-secreting cells and mean PRL secretion per cell to 52·2 and 72·0% respectively.These results suggest that (1) activin A has effects on female rat pituitary cells that increase not only the amount of FSH secretion per cell but also the number of FSHsecreting cells, and (2) activin A decreases both the amount of PRL secretion per cell and the number of PRL-secreting cells.

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Regulation of Immunoreactive Activin A Secretion from Cultured Rat Anterior Pituitary Cells.

Cited by 22 publications

References 30 publications

Effects of LHRH, FSH and Activin A on Follistatin Secretion from Cultured Rat Anterior Pituitary Cells.

Effects of LHRH, FSH and Activin A on Follistatin Secretion from Cultured Rat Anterior Pituitary Cells.

Effects of immunization against GnRH on gonadotropins, the GH-IGF-I-axis and metabolic parameters in barrows

Effects of activin on hormone secretion by single female rat pituitary cells: analysis by cell immunoblot assay

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