Regulation of inflammatory response by LINC00346 via miR-25-3p-mediated modulation of the PTEN/PI3K/AKT/NF-κB pathway

Kim, Min-Ji; Lim, Su-Geun; Cho, Dong-Hyung; Lee, Jun-Yeong; Suk, Kyoungho; Lee, Won-Ha

doi:10.1016/j.bbrc.2024.149828

Cited by 4 publications

(2 citation statements)

References 55 publications

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“…Based on a previous RNA sequencing conducted in our laboratory examining THP1 cells stimulated with LPS, several lncRNAs were revealed to be differentially expressed [21]. Out of these, the expression of LINC01270, as reported by The Genotype-Tissue Expression (GTEx) Portal, is upregulated in the spleen and whole blood (Figure 1A).…”

Section: Linc01270 Attenuation Upregulates Pro-inflammatory Cytokines...mentioning

confidence: 87%

LINC01270 Regulates the NF-κB-Mediated Pro-inflammatory Response via the miR-326/LDOC1 Axis in THP-1 Cells

Arab,

Lim,

Suk

et al. 2024

Preprint

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Long intergenic noncoding (LINC)01270 is a 2278 bp transcript belonging to the intergenic subset of long noncoding (lnc)RNAs. Despite increased reports of LINC01270’s involvement in different diseases, evident research on its effects on inflammation is yet to be achieved. In the present study, we investigated the potential role of LINC01270 in modulating the inflammatory response in the human monocytic leukemia cell line THP-1. Lipopolysaccharide treatment upregulated LINC01270 expression, and siRNA-mediated suppression of LINC01270 enhanced NF-kB activity and the subsequent production of cytokines IL-6, IL-8, and MCP-1. Interestingly, the knockdown of LINC01270 downregulated expression of leucine zipper downregulated in cancer 1 (LDOC1), a novel NF-kB suppressor. An analysis of the LINC01270/micro-RNA (miRNA)/protein interactome profile identified miR-326 as a possible mediator. Synthetic RNA agents that perturb the interaction among LINC01270, miR-326, and LDOC1 mRNA mitigated the changes caused by LINC01270 knockdown in THP-1 cells. Additionally, a luciferase re-porter assay in HEK293 cells further confirmed that LINC01270 knockdown enhances NF-kB activation, while its overexpression has the opposite effect. This study provides insight into LINC01270’s role in modulating inflammatory responses to lipopolysaccharide stimulation in THP-1 cells via miR-326/LDOC1 axis which negatively regulates NF-kB activation.

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Section: Linc01270 Attenuation Upregulates Pro-inflammatory Cytokines...mentioning

confidence: 87%

LINC01270 Regulates the NF-κB-Mediated Pro-inflammatory Response via the miR-326/LDOC1 Axis in THP-1 Cells

Arab,

Lim,

Suk

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Extensive research has confirmed that exosomes play a crucial role in intercellular communication by transporting miRNAs and proteins and have been found to reduce I/R-induced damage ( 53 , 59 , 60 ). Studies have reported that miR-25-3p in BMSC-Exo ameliorates liver I/R injury through PTEN ( 61 , 62 ). Exosomal miR-25-3p derived from MSCs has been shown to alleviate myocardial infarction by targeting proapoptotic proteins and enhancer of zeste homolog 2 ( 48 ).…”

Section: Discussionmentioning

confidence: 99%

BMSC‑derived exosome‑mediated miR‑25‑3p delivery protects against myocardial ischemia/reperfusion injury by constraining M1‑like macrophage polarization

Du,

Dong,

Song

et al. 2024

Mol Med Rep

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New insights into the function and therapeutic potential of RNA-binding protein TRBP in viral infection, chronic metabolic diseases, brain disorders and cancer

Ji,

Li,

et al. 2024

Life Sciences

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Regulation of inflammatory response by LINC00346 via miR-25-3p-mediated modulation of the PTEN/PI3K/AKT/NF-κB pathway

Cited by 4 publications

References 55 publications

LINC01270 Regulates the NF-κB-Mediated Pro-inflammatory Response via the miR-326/LDOC1 Axis in THP-1 Cells

LINC01270 Regulates the NF-κB-Mediated Pro-inflammatory Response via the miR-326/LDOC1 Axis in THP-1 Cells

BMSC‑derived exosome‑mediated miR‑25‑3p delivery protects against myocardial ischemia/reperfusion injury by constraining M1‑like macrophage polarization

New insights into the function and therapeutic potential of RNA-binding protein TRBP in viral infection, chronic metabolic diseases, brain disorders and cancer

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