Regulation of lipid droplets via the PLCβ2-PKCα-ADRP pathway in granulosa cells exposed to cadmium

Liu, Qunxing; Liang, Yuqing; Gao, Ning; Gao, Jun; Wang, Youjin; Li, Xin; Qin, Jianxiang; Xiang, Qi; Wu, Xiaoping; Chen, Hongxia; Huang, Yadong; Zhang, Qihao

doi:10.1016/j.envpol.2020.115541

Cited by 9 publications

(3 citation statements)

References 45 publications

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“…[10][11][12] Recently, ADRP has been reported as a prognostic indicator of specific cancer-containing LDs, other research has shown that ADRP-regulated LDs are involved in the steroidogenesis of ovarian granulosa cells, which all suggests that ADRP is a specific regulator of lipid homeostasis in diverse cell types and act as a targeted protein in related diseases. [13][14][15] Our previous study has confirmed the protective role of estrogen against abdominal obesity in ovariectomized mice. 16 However, it remains unknown whether ADRP is the target protein in tissue-specific regulation of estrogen in adipose tissue.…”

Section: Introductionmentioning

confidence: 56%

“…Several studies have demonstrated that ADRP exerts its adipogenic effect on the triglyceride accumulation in LDs, leading to the onset of fatty liver, insulin resistance, and atherosclerosis 10–12 . Recently, ADRP has been reported as a prognostic indicator of specific cancer‐containing LDs, other research has shown that ADRP‐regulated LDs are involved in the steroidogenesis of ovarian granulosa cells, which all suggests that ADRP is a specific regulator of lipid homeostasis in diverse cell types and act as a targeted protein in related diseases 13–15 …”

Section: Introductionmentioning

confidence: 99%

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Elevated adipose differentiation‐related protein level in ovariectomized mice correlates with tissue‐specific regulation of estrogen

Zhu

Zhang

et al. 2023

J of Obstet and Gynaecol

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Objective Redistribution of adipose tissue in the abdomen during the menopausal transition is attributable mostly to estrogen drop with aging. Adipose differentiation‐related protein (ADRP), a major component of lipid droplets, is closely related to the onset of lipid accumulation. We hypothesized that estrogen exerted its tissue‐specific effect in reducing abdominal fat accumulation by regulation of ADRP. Methods Twenty‐four female C57/BL6 mice aged 8 weeks were randomly divided into 3 groups: sham operation (Sham), bilateral ovariectomy (OVX), and OVX plus 17β‐estradiol (OVX + E2). After being fed 8 weeks of a high‐fat diet, plasma lipid profiles including total cholesterol (TC), total triglyceride (TG), low‐density lipoprotein cholesterol (LDL‐C), and high‐density lipoprotein cholesterol (HDL‐C) levels, body weight gain, visceral, and subcutaneous adipose tissue, adipocyte size, and ADRP expression were measured. Results In comparison to sham‐operated mice, OVX mice presented a weight gain with higher plasma TC, TG, LDL‐C levels, and lower HDL‐C levels. E2 supplement ameliorated the increase in weight and lipid profiles. Elevated ADRP expression was observed in visceral adipose tissue of OVX mice, whereas treatment of estrogen suppressed the ADPR expression and reversed the fat accumulation in the abdomen. However, no significant difference of ADRP expression in subcutaneous adipose tissue was detected between sham, OVX, and OVX + E2 mice. Conclusions Our findings suggested that enhanced ADRP expression in ovariectomized mice correlates with the tissue‐specific regulation of estrogen, which may provide useful clues for further exploring the regulatory mechanism and corresponding anti‐abdominal obesity treatment in postmenopausal women.

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Section: Introductionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Elevated adipose differentiation‐related protein level in ovariectomized mice correlates with tissue‐specific regulation of estrogen

Zhu

Zhang

et al. 2023

J of Obstet and Gynaecol

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“…Cd 2+ also caused the decrease of respiratory function in the freshwater crab Barytelphusa gureini, gill hyperplasia and degenerative changes in visceral organs, elevation in MDA and attenuation in GSH activity in Oreochromis niloticus (Abu-Elala et al 2021). Exposure to Cd 2+ led to the changes of protein metabolism and energy imbalances with a decrease in tissue glycogen and an elevated haemolymph glucose in snail ( Liu et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Cadmium-induced DAG-PKC/IP3-Ca2+ double signaling pathway and cell injury in gill of freshwater crabs Sinopotamon henanense

Wang,

Liu

et al. 2024

Preprint

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Cadmium (Cd) which is an extremely toxic heavy metal and widespread in the environment has been associated with numerous organs damage and even death. To evaluate the signaling pathway and the toxicity induced by Cd2+ in aquatic organisms, freshwater crabs Sinopotamon henanense were exposed to Cd2+ (0, 14.5, 29 and 58 mg/L) for 4 d. The results showed that Cd2+ induced the increase of diacylglycerol (DAG) and inositol-1,4,5-triphosphate (IP3), the rapid change of Ca2+ concentration in the cytoplasm and activation of protein kinase C (PKC) in the gill of crab. Also, Cd2+ raised the expressions of 6 genes relating with protein detoxification and folding, including cytochrome P450 (CYP450), BAG regulator 3 (BAG3), protein disulfide isomerase (PDI) and 3 genes encoding heat shock proteins (Hsp21, Hsp27 and Hsp70). And, it was observed that Cd2+ exposure aggravated filament swelling and raised the number of dead epidermal cell in gill. Meanwhile, Cd2+ exposure degraded DNA which was accompanied by a obvious decrease in ATP level, this might be the key reasons causing cell apoptosis and necrosis. In conclusion, Cd2+ triggered DAG-PKC/IP3-Ca2+ double signaling pathway, caused DNA degradation and ATP decrease, and finally induced the cell injury in S.henanense based on histological observation. This study provided more information to know the signaling events underlying Cd2+-induced cytotoxicity.

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CORO1A regulates lipoprotein uptake in Leydig cells exposed to cadmium

Wang

et al. 2022

Ecotoxicology and Environmental Safety

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Regulation of lipid droplets via the PLCβ2-PKCα-ADRP pathway in granulosa cells exposed to cadmium

Cited by 9 publications

References 45 publications

Elevated adipose differentiation‐related protein level in ovariectomized mice correlates with tissue‐specific regulation of estrogen

Elevated adipose differentiation‐related protein level in ovariectomized mice correlates with tissue‐specific regulation of estrogen

Cadmium-induced DAG-PKC/IP3-Ca2+ double signaling pathway and cell injury in gill of freshwater crabs Sinopotamon henanense

CORO1A regulates lipoprotein uptake in Leydig cells exposed to cadmium

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