Regulation of lung oxidative damage by endogenous superoxide dismutase in sepsis

Constantino, Larissa; Gonçalves, Renata C.; Giombelli, Vinícius Renê; Tomasi, Cristiane Damiani; Vuolo, Francieli; Kist, Luiza Wilges; Oliveira, Giovanna Medeiros Tavares de; Pasquali, Matheus Augusto de Bittencourt; Bogo, Maurı́cio Reis; Mauad, Thaís; Horn, Adolfo; Melo, Karen Vieira; Fernandes, Christiane; Moreira, José Cláudio Fonseca; Ritter, Cristiane

doi:10.1186/2197-425x-2-17

Cited by 24 publications

(22 citation statements)

References 27 publications

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“…Sepsis causes an uneven increase in the relation of SOD and catalase, which exacerbates oxidative stress (Visner et al 1990, Andrades et al 2005, Andrades et al 2011. In this study, all horses showed immunostaining for SOD2 in organs, and horses from the OLG showed increased immunostaining for SOD2, which corroborates the findings of increase of this antioxidant in systemic inflammatory response, especially if caused by sepsis (Constantino et al 2014, Gimenez--Garzo et al 2015. SOD2 present in hoof laminar tissue of horses in this study was observed in different intensities variables between animals, and no difference was observed among experimental groups.…”

Section: Immunostaining For Sodsupporting

confidence: 79%

“…Oxidative stress has been implicated in the development of tissue injury in several diseases, such as kidney injury as a result of experimental sepsis (Pathak et al 2012), organ failure caused by hemorrhagic shock in kidney, lungs, liver and intestine (Mota-Filipe 1990), and acetaminophen hepatotoxicity (Knight et al 2002). Peroxynitrite, a powerful oxidant agent, can be associated to oxidative lesion by the nitrotyrosine immunostaining in tissues, as observed by Constantino et al (2014) in the lungs of rats submitted to experimental sepsis. Peroxynitrite is formed by the reaction between nitric oxide and superoxide anions ) and promotes endothelial dysfunction, vascular hyporeactivity, primary myocardial disease (Szabó 1996) and inactivation of mitochondrial electron transport, damaging cellular respiration (Radi et al 1994).…”

Section: Immunostaining For Sodmentioning

confidence: 99%

“…The increase of this substance occurs after, probably in an attempt to reduce the deleterious effects of oxidizing species released (Gimenez-Garzo et al 2015). Nitrotyrosine and SOD levels increased after sepsis induction in rats, but the antioxidant effect is insufficient to protect the lungs against severe sepsis (Constantino et al 2014) The aim of this study was to evaluate the severity of laminar lesions in horses with systemic oxidative stress, affected by naturally occurring or experimentally induced gastrointestinal disorders.…”

Section: Introductionmentioning

confidence: 99%

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Laminar lesions in horses with systemic oxidative stress, committed by experimentally induced or naturally occurring gastrointestinal disorders

et al. 2016

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Laminitis in horses can be associated with lesions in multiple organs secondary to sepsis. Twenty-one horses suffering from gastrointestinal disorders were used in the experiment; 7 horses with experimentally induced endotoxemia and intestinal ischaemia, and 14 horses suffering from naturally occurring colic syndrome. Tissue samples of lungs, liver, heart, brain, cerebellum and hoof laminar tissue were collected for histopathological and oxidative stress evaluation using nitrotyrosine and superoxide dismutase (SOD2) immunostaining. The horses were divided into two groups: the non-oxidative lesions group (NOLG), with 7 horses showing weak immunostaining in lungs, liver and kidney, and the oxidative lesions group (OLG), with 14 horses showing immunostaining indicating systemic oxidative stress in multiple organs. The horses from OLG showed increase of laminar lesions and SOD2 immunostaining in multiple organs when compared to the horses from the NOLG. No differences were found ln regard to laminar immunostaining by nitrotyrosine and SOD2 between experimental groups. It was concluded that systemic oxidative stress can be associated with the development of laminar lesions, and that the laminar tissue does not respond to oxidative stress with increase of SOD as occurs in other organs.

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Section: Immunostaining For Sodsupporting

confidence: 79%

Section: Immunostaining For Sodmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Laminar lesions in horses with systemic oxidative stress, committed by experimentally induced or naturally occurring gastrointestinal disorders

et al. 2016

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“…antitumoral, antibacterial and antioxidant). [19][20][21][22][23] The main difference between those previously reported ligands (HPClNOL and H 2 BPClNOL) and…”

Section: Accepted Manuscriptmentioning

confidence: 96%

Induction of apoptosis in leukemia cell lines by new copper(II) complexes containing naphthyl groups via interaction with death receptors

Fernandes

Horn

Lopes

et al. 2015

Journal of Inorganic Biochemistry

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The synthesis, physico-chemical characterization and cytotoxicity of four new ligands and their respective copper(II) complexes toward two human leukemia cell lines (THP-1 and U937) are reported (i.e. [(HL1)Cu(μ-Cl)2Cu(HL1)]Cl2·H2O (1), [(H2L2)Cu(μ-Cl)2Cu(H2L2)]Cl2·5H2O (2), [(HL3)Cu(μ-Cl)2Cu(HL3)]Cl2·4H2O (3), [(H2L4)Cu(μ-Cl)2Cu(H2L4)]Cl2·6H2O (4)). Ligands HL1 and HL3 contain two pyridines, amine and alcohol moieties with a naphthyl pendant unit yielding a N3O coordination metal environment. Ligands H2L2 and H2L4 have pyridine, phenol, amine and alcohol groups with a naphthyl pendant unit providing a N2O2 coordination metal environment. These compounds are likely to be dinuclear in the solid state but form mononuclear species in solution. The complexes have an antiproliferative effect against both leukemia cell lines; complex (2) exhibits higher activity than cisplatin against U937 (8.20 vs 16.25μmoldm(-3)) and a comparable one against THP-1. These human neoplastic cells are also more susceptible than peripheral blood mononuclear cells (PBMCs) toward the tested compounds. Using C57BL/6 mice an LD50 of 55mgkg(-1) was determined for complex (2), suggesting that this compound is almost four times less toxic than cisplatin (LD50=14.5mgkg(-1)). The mechanism of cell death promoted by ligand H2L2 and by complexes (2) and (4) was investigated by a range of techniques demonstrating that the apoptosis signal triggered at least by complex (2) starts from an extrinsic pathway involving the activation of caspases 4 and 8. This signal is amplified by mitochondria with the concomitant release of cytochrome c and the activation of caspase 9.

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“…NT levels were also positively correlated with plasma IL-1, IL-6, TNF-α, oxLDL, and CRP levels. Constantin et al [23] demonstrated that septic animals have increased levels of NT and NO, and that NT plays a prominent role in sepsis. However, it seems that NT levels are more effective in determining the severity of sepsis than are NOx levels, as NT levels were significantly higher in patients with severe sepsis than in patients with mild sepsis.…”

Section: Discussionmentioning

confidence: 99%

Oxidative stress parameters and inflammatory and immune mediators as markers of the severity of sepsis

Bavunoğlu

Genc

Konukoğlu

et al. 2016

J Infect Dev Ctries

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Introduction: Sepsis is a complex inflammatory syndrome with diverse etiology and wide spectrum of severity. The aim of this study was to investigate whether inflammatory mediators, in comparison with oxidative parameters, are associated with severity of sepsis. Methodology: Plasma neopterin, adenosine deaminase (ADA), vascular cell adhesion molecule (VCAM), intracellular adhesion molecule (ICAM), interleukin (IL)-1, IL-6, and tumor necrosis factor alpha (TNF-α), as inflammatory mediators, and serum nitric oxide (NOx), nitrotyrosine (NT), oxidized LDL (oxLDL) levels, serum paraoxonase 1 (PON1) activity, and erythrocyte glutathione (GSH) levels as oxidative stress parameters of 12 patients with mild sepsis, 25 patients with severe sepsis, and 20 healthy control subjects were evaluated. NOx, GSH levels and PON1 activity were determined by colorimetric methods, whereas neopterin, VCAM, ICAM, IL-1, IL-6, TNF-α, NT, and oxLDL levels were measured by enzyme-linked immunosorbent assay (ELISA). Results: All parameters in mild and severe sepsis were significantly different from those of healthy subjects, except ADA activities. Patients with severe sepsis exhibited higher IL-6, TNF-α, NT, and oxLDL levels than patients with mild sepsis. GSH (98%, 98%), oxLDL (98%, 98%), VCAM-1 (99%, 99%), and ICAM-1 (99%, 99%) have much more sensitivitiy and specificity in sepsis. Conclusions: Our results suggest that the oxidative stress and inflammatory response in patients with sepsis were increased and that serum IL-6, TNF-α, NT, and oxLDL levels were correlated with the severity of sepsis. Therefore, increases in these parameters may contribute to the dysfunction or failure of one or more organs, or even death, in sepsis.

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Regulation of lung oxidative damage by endogenous superoxide dismutase in sepsis

Cited by 24 publications

References 27 publications

Laminar lesions in horses with systemic oxidative stress, committed by experimentally induced or naturally occurring gastrointestinal disorders

Laminar lesions in horses with systemic oxidative stress, committed by experimentally induced or naturally occurring gastrointestinal disorders

Induction of apoptosis in leukemia cell lines by new copper(II) complexes containing naphthyl groups via interaction with death receptors

Oxidative stress parameters and inflammatory and immune mediators as markers of the severity of sepsis

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