Regulation of lysine biosynthesis by leucine in Brevibacterium lactofermentum.

Tosaka, Osamu; Hirakawa, Hayao; Takinami, Kôichi; Hirose, Yoshio

doi:10.1271/bbb1961.42.1501

Cited by 18 publications

(10 citation statements)

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“…It is well known that some industrial C. glutamicum strains used for L-lysine production are characterized by leucine auxotrophy (63,55). Mutation of the leuC gene in a rationally designed L-lysine producer resulted in significant up-regulation of many amino acid biosynthesis genes, including leuCD and the trp operon genes (19).…”

Section: Discussionmentioning

confidence: 99%

The IclR-Type Transcriptional Repressor LtbR Regulates the Expression of Leucine and Tryptophan Biosynthesis Genes in the Amino Acid ProducerCorynebacterium glutamicum

et al. 2007

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The transcriptional regulator Cg1486 of Corynebacterium glutamicum ATCC 13032 is a member of the IclR protein family and belongs to the conserved set of regulatory proteins in corynebacteria. A defined deletion in the cg1486 gene, now designated ltbR (leucine and tryptophan biosynthesis regulator), led to the mutant strain C. glutamicum IB1486. According to whole-genome expression analysis by DNA microarray hybridizations, transcription of the leuB and leuCD genes encoding enzymes of the leucine biosynthesis pathway was enhanced in C. glutamicum IB1486 compared with the wild-type strain. Moreover, the genes of the trpEGDCFBA operon involved in tryptophan biosynthesis of C. glutamicum showed an enhanced expression in the cg1486 mutant strain. Bioinformatics pattern searches in the upstream regions of the differentially expressed genes revealed the common 12-bp motif CA(T/C)ATAGTG(A/G)GA that is located downstream of the ؊10 region of the mapped promoter sequences. DNA band shift assays with a streptavidin-tagged LtbR protein demonstrated the specific binding of the purified protein to 40-mers containing the 12-bp motif localized in front of leuB, leuC, and trpE, thereby confirming the direct regulatory role of LtbR in the expression of the leucine and tryptophan biosynthesis pathway genes of C. glutamicum. Genes homologous with ltbR were detected upstream of the leuCD genes in almost all sequenced genomes of bacteria belonging to the taxonomic class Actinobacteria. The ltbR-like genes of Corynebacterium diphtheriae, Corynebacterium jeikeium, Mycobacterium bovis, and Bifidobacterium longum were cloned and shown to complement the deregulation of leuB, leuCD, and trpE gene expression in C. glutamicum IB1486.

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Section: Discussionmentioning

confidence: 99%

The IclR-Type Transcriptional Repressor LtbR Regulates the Expression of Leucine and Tryptophan Biosynthesis Genes in the Amino Acid ProducerCorynebacterium glutamicum

et al. 2007

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“…It is known that some mutants classically derived from coryneform bacteria for L-lysine production are L-leucine auxotrophs (Tosaka et al 1978). These strains accumulate considerable amounts of L-lysine, provided that L-leucine-limiting conditions are established (Patek et al 1994).…”

Section: Discussionmentioning

confidence: 99%

“…One is release from the L-leucine-dependent repression of the dapA gene encoding dihydrodipicolinate synthase which is the first enzyme of the L-lysine-biosynthetic pathway (Tosaka et al 1978). The other is the decreased biomass which might allow increased precursor supply from central metabolism into the L-lysine-biosynthetic pathway through minimizing the loss of carbon required for cell growth (Patek et al 1994).…”

Section: Discussionmentioning

confidence: 99%

A leuC mutation leading to increased L-lysine production and rel-independent global expression changes in Corynebacterium glutamicum

Hayashi

Mizoguchi

Ohnishi

et al. 2006

Appl Microbiol Biotechnol

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We previously found by transcriptome analysis that global induction of amino acid-biosynthetic genes occurs in a classically derived industrial L-lysine producer, Corynebacterium glutamicum B-6. Based on this stringent-like transcriptional profile in strain B-6, we analyzed the relevant mutations from among those identified in the genome of the strain, with special attention to the genes that are involved in amino acid biosynthesis and metabolism. Among these mutations, a Gly-456→Asp mutation in the 3-isopropylmalate dehydratase large subunit gene (leuC) was defined as a useful mutation. Introduction of the leuC mutation into a defined L-lysine producer, AHD-2 (hom59 and lysC311), by allelic replacement led to the phenotype of a partial requirement for L-leucine and approximately 14% increased L-lysine production.Transcriptome analysis revealed that many amino acid-biosynthetic genes including lysC-asd operon were significantly up-regulated in the leuC mutant in a rel-independent manner.

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“…[11][12][13] We have reported that y To whom correspondence shoud be addressed. Tel: +81-44-244-7168; Fax: +81-44-211-7833; E-mail: kouhei ishikawa@ajinomoto.com threonine inhibits aspartokinase acitivity.…”

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confidence: 99%

Disruption ofmetFIncreased L-Lysine Production byMethylophilus methylotrophusfrom Methanol

Ishikawa¹,

Asahara²,

Gunji³

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Regulation of lysine biosynthesis by leucine in Brevibacterium lactofermentum.

Cited by 18 publications

References 0 publications

The IclR-Type Transcriptional Repressor LtbR Regulates the Expression of Leucine and Tryptophan Biosynthesis Genes in the Amino Acid ProducerCorynebacterium glutamicum

The IclR-Type Transcriptional Repressor LtbR Regulates the Expression of Leucine and Tryptophan Biosynthesis Genes in the Amino Acid ProducerCorynebacterium glutamicum

A leuC mutation leading to increased L-lysine production and rel-independent global expression changes in Corynebacterium glutamicum

Disruption ofmetFIncreased L-Lysine Production byMethylophilus methylotrophusfrom Methanol

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