The relationship between L-lysine production and biotin levels in culture medium was investigated in Brevibacterium tactofermentum. Strain AJ3799 accumulated 34 mg/ml and 42 mg/ml of L-lysine•EHCl in the presence of 50ƒÊg/liter and 500ƒÊg/liter, respectively, of biotin in culture medium.
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