Regulation of mammary lipogenic genes in dairy cows fed crushed sunflower seeds

Lashkari, Saman; Moller, Jeppe W.; Theil, Peter Kappel; Weisbjerg, M.R.; Jensen, Søren Krogh; Sørensen, Martin Tang; Sejrsen, K.

doi:10.1016/j.livsci.2020.104035

Cited by 4 publications

(2 citation statements)

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“…Supplementation of dairy cow diets with FA has been studied in many experiments. It is well documented that addition of oil seed rich in UFA to the diet reduces the proportion content of endogenously (de novo) synthesized FA and increases the content of long‐chained UFA in milk (Lashkari, Moller, Theil, et al, 2020). The reduced content of de novo synthesized FA occurs concomitant with a reduced mammary transcription of genes important for de novo FA synthesis, that is lipogenic genes including stearoyl CoA dehydrogenase 1 ( SCD1 ), FA synthase ( FAS ) and sterol response element‐binding protein 1 ( SREBP1 ; Harvatine and Bauman, 2006; Peterson, Matitashvili, & Bauman, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…In dairy cows, dietary supplementation of oil seed rich in UFA increases the incorporation of UFA into phospholipids in the MFGM (Lashkari, Moller, Theil, et al, 2020; Lopez et al., 2008; Palmquist & Schanbacher, 1991). Because the MFGM is derived from the membranes of the mammary epithelial cells, the changed FA composition of the MFGM most likely reflects a general changed composition of the phospholipids in the cell from which it originates (Heid & Keenan, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Changes in long‐chain fatty acid composition of milk fat globule membrane and expression of mammary lipogenic genes in dairy cows fed sunflower seeds and rumen‐protected choline

Lashkari

Moller

Jensen

et al. 2020

Animal Physiology Nutrition

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The aim of this experiment was to investigate the effect of dietary supplementation of crushed high oleic sunflower seeds (HOS) and rumen-protected choline (RPC) on the fatty acid (FA) profile of phospholipids and sphingomyelin and mammary transcription of genes that are important for milk fat synthesis and de novo synthesis of sphingolipids. Twenty-four cows were divided into four groups that either received an unsupplemented diet (Control), the Control diet supplemented with 50 g RPC per day, a diet supplemented with HOS at 10% of dry matter, or RPC and HOS in combination (RPC + HOS). RPC supplementation had no effect on the FA composition of milk or sphingomyelin. Cows receiving RPC and RPC + HOS had increased incorporation of C22:5 (n-3) into phospholipids. Milk FA proportion of C18:0 and C18:1 isomers was increased in cows receiving HOS (HOS and RPC + HOS). Sphingomyelin proportion of C22:0 was increased in cows receiving HOS and RPC + HOS, at the expense of C23:0. HOS supplementation further increased the proportion of unsaturated fatty acids (UFA) in milk phospholipids. HOS supplementation increased mammary transcription of UDP-glucose ceramide glycosyltransferase (UGCG), sterol response element-binding protein cleavage-activating protein (SCAP) and peroxisome proliferation-activated receptor Gamma subunit C 1b (PPARGC1b), and reduced transcription of insulin induced gene 1 (INSIG1) and fatty acid-binding protein 3 (FABP3). Dietary supplementation of RPC increased mammary transcription of fatty acid desaturase 1 (FADS1) and longevity assurance gene 2 (LASS2), and reduced transcription of sphingomyelin synthase (SGMS). The results show that the FA profile of milk phospholipids is sensitive to dietary lipid supplementation and, to a minor degree, RPC supplementation. Furthermore, transcription of genes that are important for milk fat synthesis and sphingolipids synthesis is affected by dietary supplementation of RPC and HOS.

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Section: Introductionmentioning

confidence: 99%