Regulation of metabolism by dietary carbohydrates in two lines of rainbow trout divergently selected for muscle fat content

Kamalam, Biju Sam; Médale, Françoise; Kaushik, Sadasivam; Polakof, Sergio; Skiba‐Cassy, Sandrine; Panserat, Stéphane

doi:10.1242/jeb.070581

Cited by 131 publications

(149 citation statements)

References 73 publications

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“…Where both gene expression and enzyme activity have been studied, G6pase expression is often linked to G6Pase enzyme activity (Caseras et al 2002;Méton et al 2004;Saldago et al 2004). However, down regulation of G6Pase gene expression does not automatically mean inhibition of gluconeogenesis, as a recent study performed in trout showed a post-prandial inhibition of G6Pase gene expression was not linked to the modification of G6Pase enzyme activity (Skiba-Cassy et al, 2012).…”

Section: Discussionmentioning

confidence: 96%

Postprandial molecular responses in the liver of the barramundi, Lates calcarifer

Wade

Skiba‐Cassy

Dias

et al. 2013

Fish Physiol Biochem

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The regulation of gene expression by nutrients is an important mechanism governing energy storage and growth in most animals, including fish.Understanding the timing and intensity of these responses is the first critical step in defining the molecular effects of different dietary nutrient sources. In this study, changes in key metabolic regulators of nutritional pathways were investigated in barramundi fed a meal of a diet formulated with 500, 150 and 110 g kg -1 dry matter of protein, lipid and carbohydrate, respectively. Plasma glucose levels showed a postprandial peak two hours after feeding, and had returned to basal levels within four hours. Significant activation of genes that regulate glycolytic and lipogenic pathways immediately post feeding were observed, in combination with down-regulation of genes that control gluconeogenesis and activation of the Akt-mTOR pathway. Strong correlations were identified between a number of different metabolic genes, and the coordinated co-regulation of these genes may underlie the ability of this fish to effectively regulate circulating plasma glucose levels. Overall, post-prandial responses in barramundi were observed to be extremely rapid compared with other fish species, and there was no prolonged hyperglycaemia with a diet low in carbohydrates. These data clearly demonstrate, for the first time, the molecular changes that control intermediary metabolism in barramundi in response to feeding a single meal of a diet formulated within optimal specifications for this species.

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Section: Discussionmentioning

confidence: 96%

Postprandial molecular responses in the liver of the barramundi, Lates calcarifer

Wade

Skiba‐Cassy

Dias

et al. 2013

Fish Physiol Biochem

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“…Anti-phospho Akt (Ser473), anti-carboxyl terminal Akt, anti-phospho AMPK (Thr172), anti-AMPK, anti-phospho-FoxO1 (Thr24) and antiFoxO1 (L27) antibodies were used (Cell Signaling Technology, Saint Quentin Yvelings, France). All these antibodies cross-react successfully with rainbow trout proteins of interest (Skiba-Cassy et al, 2009;Kamalam et al, 2012;Velasco et al, 2016b). After washing, membranes were incubated with an IgG-HRP secondary antibody (Bio-Rad) and spots were quantified by Image Lab software version 5.2.1 (Bio-Rad).…”

Section: Western Blot Analysismentioning

confidence: 99%

Ceramide counteracts the effects of ghrelin on the metabolic control of food intake in rainbow trout

Velasco

Moreiras

Conde-Sieira

et al. 2017

Journal of Experimental Biology

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In mammals, ceramides are involved in the modulation of the orexigenic effects of ghrelin (GHRL). We previously demonstrated in rainbow trout that intracerebroventricular (ICV) treatment with ceramide (2.5 µg/100 g fish) resulted in an anorexigenic response, i.e. a response opposed to that described in mammals, where ceramide treatment is orexigenic. Therefore, we hypothesized that the putative interaction between GHRL and ceramide must be different in fish. Accordingly, in a first experiment, we observed that ceramide levels in the hypothalamus of rainbow trout did not change after ICV treatment with GHRL. In a second experiment, we assessed whether the effects of GHRL treatment on the regulation of food intake in rainbow trout changed in the presence of ceramide. Thus, we injected ICV GHRL and ceramide alone or in combination to evaluate in hypothalamus and hindbrain changes in parameters related to the metabolic control of food intake. The presence of ceramide generally counteracted the effects elicited by GHRL on fatty acid-sensing systems, the capacity of integrative sensors (AMPK, mTOR and SIRT-1), proteins involved in cellular signalling pathways (Akt and FoxO1) and neuropeptides involved in the regulation of food intake (AgRP, NPY, POMC and CART). The results are discussed in the context of regulation of food intake by metabolic and endocrine inputs.

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“…The primer sequences used in the real-time RT-PCR assays as well as the protocol conditions of the assays for mRNA of glucose and amino acid metabolic genes (Kamalam et al, 2012;Kamalam et al, 2013) and miRNAs (Mennigen et al, 2012) have previously been published. The transcripts analysed were glucokinase (GK; EC 2.7.1.2), hexokinase 1 (HK1; EC 2.7.1.1), 6-phosphofructo-1-kinase (6PFK; EC 2.7.1.11) and pyruvate kinase (Dumortier et al, 2013;Ramírez et al, 2013).…”

Section: Relative Quantification Of Mrna and Mirnamentioning

confidence: 99%

“…An amount of 1 μg of total RNA was used for cDNA synthesis. The NCode TM VILO TM miRNA cDNA Synthesis Kit (Invitrogen) or the SuperScript III RNaseH-Reverse Transcriptase Kit (Invitrogen) with random primers (Promega, Charbonniéres, France) was used according to the manufacturer's protocol to synthesize cDNA (n=9 for each time point) for miRNA and mRNA, respectively.The primer sequences used in the real-time RT-PCR assays as well as the protocol conditions of the assays for mRNA of glucose and amino acid metabolic genes (Kamalam et al, 2012;Kamalam et al, 2013) and miRNAs (Mennigen et al, 2012) have previously been published. The transcripts analysed were glucokinase (GK; EC 2.7.1.2), hexokinase 1 (HK1; EC 2.7.1.1), 6-phosphofructo-1-kinase (6PFK; EC 2.7.1.11) and pyruvate kinase (Dumortier et al, 2013;Ramírez et al, 2013).…”

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confidence: 99%

High or low dietary carbohydrate:protein ratios during first-feeding affect glucose metabolism and intestinal microbiota in juvenile rainbow trout

Geurden

Mennigen

Plagnes-Juan

et al. 2014

Journal of Experimental Biology

103

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Based on the concept of nutritional programming in mammals, we tested whether an acute hyperglucidic-hypoproteic stimulus during first feeding could induce long-term changes in nutrient metabolism in rainbow trout. Trout alevins received during the five first days of exogenous feeding either a hyperglucidic (40% gelatinized starch + 20% glucose) and hypoproteic (20%) diet (VLP diet) or a high-protein (60%) glucose-free diet (HP diet, control). Following a common 105-day period on a commercial diet, both groups were then challenged (65 days) with a carbohydrate-rich diet (28%). Short-and long-term effects of the early stimuli were evaluated in terms of metabolic marker gene expressions and intestinal microbiota as initial gut colonisation is essential for regulating the development of the digestive system. In whole alevins (short term), diet VLP relative to HP rapidly increased gene expressions of glycolytic enzymes, while those involved in gluconeogenesis and amino acid catabolism decreased. However, none of these genes showed persistent molecular adaptation in the liver of challenged juveniles (long term). By contrast, muscle of challenged juveniles subjected previously to the VLP stimulus displayed downregulated expression of markers of glycolysis and glucose transport (not seen in the short term). These fish also had higher plasma glucose (9 h postprandial), suggesting impaired glucose homeostasis induced by the early stimulus. The early stimulus did not modify the expression of the analysed metabolism-related microRNAs, but had short-and long-term effects on intestinal fungi (not bacteria) profiles. In summary, our data show that a short hyperglucidic-hypoproteic stimulus during early life may have a long-term influence on muscle glucose metabolism and intestinal microbiota in trout. KEY WORDS: Nutritional programming, Rainbow trout, Carbohydrates, Protein, Metabolism INTRODUCTIONPrenatal or early nutritional neonatal events exerted during critical developmental windows may result in permanent changes in postnatal growth potential, health and metabolic status in mammals (Burdge and Lillycrop, 2010;Lucas, 1998;Patel and Srinivasan, 2002;Patel et al., 2009;Metges et al., 2014;Duque-Guimarães and Ozanne, 2013;Devaskar and Thamotharan, 2007). It has been RESEARCH ARTICLE 1 INRA, UR1067 Nutrition Metabolism and Aquaculture, F-64310 Saint-Pée-surNivelle, France. suggested that this process of developmental plasticity utilizes the early nutritional cues to prepare individual phenotypes to better match the predicted future nutritional environment (Gluckman et al., 2005). Possible biological mechanisms for 'imprinting' the nutritional event until adulthood in mammalian vertebrates comprise adaptive changes in gene expression pattern or cellular phenotype (epigenetic phenomenon), nutrient-sensitive signalling pathways and adaptive clonal selection, which may be transmitted to future offspring (Lucas, 1998;Symonds et al., 2009;Waterland and Jirtle, 2003;Gut and Verdin, 2013). Experimental data on the concept...

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Regulation of metabolism by dietary carbohydrates in two lines of rainbow trout divergently selected for muscle fat content

Cited by 131 publications

References 73 publications

Postprandial molecular responses in the liver of the barramundi, Lates calcarifer

Postprandial molecular responses in the liver of the barramundi, Lates calcarifer

Ceramide counteracts the effects of ghrelin on the metabolic control of food intake in rainbow trout

High or low dietary carbohydrate:protein ratios during first-feeding affect glucose metabolism and intestinal microbiota in juvenile rainbow trout

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