Regulation of metallothionein-3 mRNA by thyroid hormone in developing rat brain and primary cultures of rat astrocytes and neurons

Yeiser, E.Carden; Fitch, Cheryl A.; Horning, Michelle S.; Rutkoski, Nancy J.; Levenson, Cathy W.

doi:10.1016/s0165-3806(99)00063-2

Cited by 18 publications

(14 citation statements)

References 40 publications

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“…Distinct increase in expression of all four MTs during differentiation from immature NSPCs to their progeny in vitro (Figs. 1B and 3E-H) well corresponds to the knowledge that higher level of MTs was expressed in adult brain than the embryonic brain or the brain of the newborn rat (Choudhuri et al, 1996;Yeiser et al, 1999) in vivo. In the CNS development, ratio of immature NSPCs is high because of their active proliferation in the embryonic stage but decrease in the post natal brain.…”

Section: Discussionsupporting

confidence: 79%

Reduced zinc cytotoxicity following differentiation of neural stem/progenitor cells into neurons and glial cells is associated with upregulation of metallothioneins

Nishikawa¹,

Mori²,

Hara³

2015

Environmental Toxicology and Pharmacology

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Section: Discussionsupporting

confidence: 79%

Reduced zinc cytotoxicity following differentiation of neural stem/progenitor cells into neurons and glial cells is associated with upregulation of metallothioneins

Nishikawa¹,

Mori²,

Hara³

2015

Environmental Toxicology and Pharmacology

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“…MT-3 expression can be altered by various cytokines and hormones, including thyroxin. Even sleep deprivation has been reported to have effects on MT-3 Table II expression (10,11). It is, therefore, possible that factors other than the disease may have some influence on MTs expression, thus resulting in some variations in MTs expression among control patients.…”

Section: Discussionmentioning

confidence: 99%

The expression of metallothioneins is diminished in the spinal cords of patients with sporadic ALS

Hozumi

Yamada

Uchida

et al. 2008

Amyotrophic Lateral Sclerosis

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We analyzed the expression of MTs using immunohistochemistry on the spinal cords of patients with ALS (n =12) and controls (n =12). The immunoreactivities of both MT-1/2 and MT-3 stained dominantly in glial cells and were decreased in the spinal cords of patients with ALS, particularly in patients on respirators. The immunoreactivity of MT-1/2 in the ALS groups was significantly reduced compared with controls. In addition, a statistical analysis revealed that the immunoreactivity of MT-3 in astrocytes in the gray matter of the lumbar spinal cord was negatively correlated with the duration of ALS. Both MT-1/2 and MT-3 immunoreactivities were detected mainly in the glias and also detected in some neurons in both control patients and patients with ALS. Interestingly, the patients with MT-3-positive neurons showed definite MT-3-immunoreactive glial reaction around neurons. Previous studies have reported that familial ALS (FALS) model mice (G93A SOD1) crossed with MT-1/2 or MT-3 knock-out mice had accelerated expression of ALS. Judged from these findings, both MT-1/2 and MT-3 play important roles in the progression of ALS. MTs are defensive proteins that can scavenge free radicals; therefore, manipulation of their expression has a strong therapeutic potential for ALS patients.

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“…It belongs to a family of low molecular weight and cysteine-rich proteins with high affinity for heavy metal ions (Margoshes and Vallee, 1957). Human MT genes are located on chromosome 16 and are classified as four isoforms: MT-1 and -2 are expressed ubiquitously, MT-3 is expressed in neural tissues, and MT-4 is expressed in the stratified squamous epithelia (Stennard et al, 1994;Yeiser et al, 1999;Quaife et al, 1994). MT-1 and MT-2 expression is induced by cadmium, zinc, and copper, and these MT isoforms protect against cytotoxicity induced by these metals (Kägi, 1991;Sato and Bremmer, 1993).…”

Section: Introductionmentioning

confidence: 99%

Induction of metallothionein isoforms by copper diethyldithiocarbamate in cultured vascular endothelial cells

et al. 2016

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-Metallothionein (MT) plays a central role in cellular defense against heavy metals and oxidative stress. Since the induction of MT requires the activation of metal response element (MRE)-binding transcription factor-1 (MTF-1) by binding of zinc ions, inorganic zinc is regarded as a typical MT inducer. However, in a previous report, we showed that inorganic zinc could not induce MT in vascular endothelial cells. While it is suggested that endothelial MT presents mechanisms different from those of other cell types, these remain unclear. In this study, we investigated whether the induction of endothelial MT expression involves the Nrf2-ARE pathway using copper(II) bis(diethyldithiocarbamate), termed Cu10, using a culture system of bovine aortic endothelial cells. Cu10 induced MT-1/2 protein expression and increased the expression of mRNAs for MT-1A, MT-1E, and MT-2, MT isoforms expressed in the cells. Cu10 activated not only the MTF-1-MRE, but also the Nrf2-ARE pathway. MTF-1 knockdown resulted in the repression of Cu10-induced MT-1 and -2 expression. Cu10-induced MT-1 expression was down-regulated by Nrf2 knockdown. However, MT-2 expression was not affected by Nrf2 knockdown. These results suggest that the expression of endothelial MT is up-regulated by the Nrf2-ARE pathway as well as by the MTF-1-MRE pathway. Moreover, MT-1 regulation mechanisms differ from that of MT-2. Specifically, the present data support the hypothesis that MT-1 participates in the biological defense system, while MT-2 mainly regulates intracellular zinc metabolism.

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Regulation of metallothionein-3 mRNA by thyroid hormone in developing rat brain and primary cultures of rat astrocytes and neurons

Cited by 18 publications

References 40 publications

Reduced zinc cytotoxicity following differentiation of neural stem/progenitor cells into neurons and glial cells is associated with upregulation of metallothioneins

Reduced zinc cytotoxicity following differentiation of neural stem/progenitor cells into neurons and glial cells is associated with upregulation of metallothioneins

The expression of metallothioneins is diminished in the spinal cords of patients with sporadic ALS

Induction of metallothionein isoforms by copper diethyldithiocarbamate in cultured vascular endothelial cells

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