Regulation of micoRNA2111 and its target IbFBK in sweet potato on wounding

Weng, Shiau-Ting; Kuo, Yun‐Wei; King, Yu‐Chi; Lin, Hsin-Hung; Tu, Pin-Yang; Tung, Kuei-Shu; Jeng, Shih‐Tong

doi:10.1016/j.plantsci.2019.110391

Cited by 6 publications

(3 citation statements)

References 61 publications

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“…This is also consistent with our GO classification of differentially expressed mRNAs, which were significantly enriched in photosynthesis (GO: 0015979), carbohydrate metabolic process (GO: 0005975), and disaccharide metabolic process (GO: 0005984) and pathways of photosynthesis, glucose metabolism, and glycosyl hydrolysis. Anthocyanins accumulated, chlorophyll degraded, anthocyanin/ chlorophyll values increases, and the pentagonal maple leaves turned red (Weng et al, 2020), which is consistent with the discoloration mechanism of many purple-red leaves. In the discoloration reaction dominated by anthocyanins, the color difference is related to the anthocyanin content, and the different glycoside types combined with the same anthocyanins will also lead to differences in leaf color (Matthews et al, 2003).…”

Section: B a Figuresupporting

confidence: 75%

Regulatory mechanisms and metabolic changes of miRNA during leaf color change in the bud mutation branches of Acer pictum subsp. mono

Lin

Ma²,

Zhang³

et al. 2023

Front. Plant Sci.

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Acer pictum subsp. mono is a colorful tree species with considerable ornamental and economic value. However, little is known about the metabolism and regulatory mechanism of leaf color change in A. p. subsp. mono. To reveal the molecular mechanism of leaf color change in A. p. subsp. mono, the present study examined the bud mutation branches and compared the metabolites of the red leaves (AR) of the bud mutation branches of A. p. subsp. mono with those of the green leaves (AG) of the wild-type branches. It was found that the chlorophyll and carotenoids content of the red leaves decreased significantly, while anthocyanins, and various antioxidant enzymes increased significantly compared with the green leaves. The glycosides cyanidin, pelargonidin, malvidin, petunidin, delphinidin, and peonidin were detected in AR by liquid chromatography-mass spectrometry. The cyanidin glycosides increased, and cyanidin 3-O-glycoside was significantly upregulated. We analyzed the transcriptome and small RNA of A. p. subsp. mono leaves and detected 4061 differentially expressed mRNAs and 116 differentially expressed miRNAs. Through miRNA-mRNA association analysis, five differentially expressed modules were found; one miRNA targeted three genes, and four miRNAs targeted a single gene. Among them, miR160b, miR6300, and miR396g were found to be the key miRNAs regulating stable anthocyanin accumulation in A. p. subsp. mono leaves. By revealing the physiological response of leaf color change and the molecular regulatory mechanism of the miRNA, this study provides new insight into the molecular regulatory mechanism of leaf color change, thereby offering a foundation for future studies.

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Section: B a Figuresupporting

confidence: 75%

Regulatory mechanisms and metabolic changes of miRNA during leaf color change in the bud mutation branches of Acer pictum subsp. mono

Lin

Ma²,

Zhang³

et al. 2023

Front. Plant Sci.

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“…Although sweet potato is a root crop that is highly important for food security [ 51 ], research on sweet potato has lagged behind other crops, such as rice, wheat, and maize, because of the complexity of the genome and the inefficiency of genetic transformation. So far, the only functionally characterized miRNAs in sweet potato are IbmiR2111 [ 52 ], IbmiR828 [ 53 ], and IbmiR408 [ 54 ]. In the present study, we found that IbmiR319a negatively regulated the abundance of IbTCP11/17 mRNA, which affected plant architecture and drought tolerance in sweet potato.…”

Section: Discussionmentioning

confidence: 99%

Blocking IbmiR319a Impacts Plant Architecture and Reduces Drought Tolerance in Sweet Potato

Ren

Zhang

et al. 2022

Genes

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MicroRNA319 (miR319) plays a key role in plant growth, development, and multiple resistance by repressing the expression of targeted TEOSINTE BRANCHED/CYCLOIDEA/PCF (TCP) genes. Two members, IbmiR319a and IbmiR319c, were discovered in the miR319 gene family in sweet potato (Ipomoea batatas [L.] Lam). Here, we focused on the biological function and potential molecular mechanism of the response of IbmiR319a to drought stress in sweet potato. Blocking IbmiR319a in transgenic sweet potato (MIM319) resulted in a slim and tender phenotype and greater sensitivity to drought stress. Microscopic observations revealed that blocking IbmiR319a decreased the cell width and increased the stomatal distribution in the adaxial leaf epidermis, and also increased the intercellular space in the leaf and petiole. We also found that the lignin content was reduced, which led to increased brittleness in MIM319. Quantitative real-time PCR showed that the expression levels of key genes in the lignin biosynthesis pathway were much lower in the MIM319 lines than in the wild type. Ectopic expression of IbmiR319a-targeted genes IbTCP11 and IbTCP17 in Arabidopsis resulted in similar phenotypes to MIM319. We also showed that the expression of IbTCP11 and IbTCP17 was largely induced by drought stress. Transcriptome analysis indicated that cell growth-related pathways, such as plant hormonal signaling, were significantly downregulated with the blocking of IbmiR319a. Taken together, our findings suggest that IbmiR319a affects plant architecture by targeting IbTCP11/17 to control the response to drought stress in sweet potato.

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“…However, the protein targets of TML have not been described up to date. Interestingly, in sweet potato Ipomoea batatas, miRNA2111 was found to be involved in wound response, and a wound-inducible gene, IbFBK (F-box/kelch repeat protein), was identified as its target [173]. Moreover, it was found that the kelch-repeat domain of IbFBK interacts with IbCNR8 (CELL NUMBER REGULATOR 8), which was suggested to lead to the ubiquitination and degradation of IbCNR8.…”

Section: Players Of Systemic Response To Nutrient Availability and Their Role In Nodulationmentioning

confidence: 99%

At the Root of Nodule Organogenesis: Conserved Regulatory Pathways Recruited by Rhizobia

Lebedeva

Azarakhsh

Sadikova

et al. 2021

Plants

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The interaction between legume plants and soil bacteria rhizobia results in the formation of new organs on the plant roots, symbiotic nodules, where rhizobia fix atmospheric nitrogen. Symbiotic nodules represent a perfect model to trace how the pre-existing regulatory pathways have been recruited and modified to control the development of evolutionary “new” organs. In particular, genes involved in the early stages of lateral root development have been co-opted to regulate nodule development. Other regulatory pathways, including the players of the KNOX-cytokinin module, the homologues of the miR172-AP2 module, and the players of the systemic response to nutrient availability, have also been recruited to a unique regulatory program effectively governing symbiotic nodule development. The role of the NIN transcription factor in the recruitment of such regulatory modules to nodulation is discussed in more details.

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Regulation of micoRNA2111 and its target IbFBK in sweet potato on wounding

Cited by 6 publications

References 61 publications

Regulatory mechanisms and metabolic changes of miRNA during leaf color change in the bud mutation branches of Acer pictum subsp. mono

Regulatory mechanisms and metabolic changes of miRNA during leaf color change in the bud mutation branches of Acer pictum subsp. mono

Blocking IbmiR319a Impacts Plant Architecture and Reduces Drought Tolerance in Sweet Potato

At the Root of Nodule Organogenesis: Conserved Regulatory Pathways Recruited by Rhizobia

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