Regulation of microRNAs in coronary Atherosclerotic Plaque

Abstract

Aim: Identification of microRNAs (miRNAs) associated with atherosclerosis may unravel novel therapeutic targets and biomarkers. We studied miRNAs differentially expressed between coronary atherosclerotic plaques (CAP) and healthy arteries. Materials & methods: Paired CAP and internal mammary arteries (IMA) were collected from 14 coronary artery disease patients. The miRNA profiles between diseased (CAP) and healthy (IMA) tissues were compared using microarrays and quantitative PCR. Results: Thirty-one miRNAs were differentially expressed between CAP and IMA. Among these, miR-486-5p showed a high level of regulation (12-fold), had predicted interactions with atherosclerosis-associated genes and correlated with triglyceride levels and arterial stenosis. Regulation of miR-486-5p was validated by PCR (p = 0.004). Conclusion: The miRNAs are regulated in the atherosclerotic plaque. We highlight miR-486-5p whose role in atherosclerosis requires further investigation.

circRNA‑0006896‑miR1264‑DNMT1 axis plays an important role in carotid plaque destabilization by regulating the behavior of endothelial cells in atherosclerosis

“…Previous studies have been conducted to identify therapeutic targets for UA, most of which have been limited to protein-coding genes, miRNAs and lncRNAs (32)(33)(34)(35). Recently, circRNAs have attracted attention as new diagnostic markers for diseases, including cancer.…”

Recognized and Potentially New Biomarkers—Their Role in Diagnosis and Prognosis of Cardiovascular Disease

“…miR-21 high abundance was observed in macrophages [ 70 ] and was revealed to affect foam cell formation [ 71 ]. Berkan et al found that the formation of atherosclerotic plaque was associated with miR-486-5p downregulation [ 72 ].…”

“…Regulation of microRNAs in coronary atherosclerotic plaque [72] miR-486-5p Formation of atherosclerotic plaque is associated with miR-486-5p downregulation. Circulating miR-146a may be a potential biomarker of coronary heart disease in patients with subclinical hypothyroidism [73] miR-146a miR-146a is a possible predictor of atherosclerosis and its severity in patients with hypothyroidism and raised TSH level.…”

“…Regulation of microRNAs in coronary atherosclerotic plaque [72] miR-486-5p Formation of atherosclerotic plaque is associated with miR-486-5p downregulation.…”

A Year in the Life of the EU-CardioRNA COST Action: CA17129 Catalysing Transcriptomics Research in Cardiovascular Disease

“…Scientific publications published on behalf of the EU-CardioRNA COST Action included two or more member authors from at least two different COST member states or partner countries. Three scientific papers, in addition to the previously mentioned EU-CardioRNA project report, had been published (until 16 October 2019) [30][31][32].…”