Regulation of Mitochondrial Gene Expression in Saccharomyces cerevisiae

Dieckmann, Carol L.; Staples, Robin R.

doi:10.1016/s0074-7696(08)62556-5

Cited by 84 publications

(47 citation statements)

References 193 publications

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“…Such factors have been described for the three mitochondrially encoded subunits of cytochrome oxidase, cytochrome b and subunits of the proton translocating ATPase or F 1 -F 0 complex (reviewed in Costanzo and Fox, 1990;Dieckmann and Staples, 1994). Two nuclear genes are known to be required for expression of Atp9p (subunit 9 or subunit c) of F 0 , the proton transclocating sector of the ATPase complex.…”

Section: Introductionmentioning

confidence: 99%

ATP25, a New Nuclear Gene ofSaccharomyces cerevisiaeRequired for Expression and Assembly of the Atp9p Subunit of Mitochondrial ATPase

Zeng

Barros

Shul'man

et al. 2008

MBoC

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We report a new nuclear gene, designated ATP25 (reading frame YMR098C on chromosome XIII), required for expression of Atp9p (subunit 9) of the Saccharomyces cerevisiae mitochondrial proton translocating ATPase. Mutations in ATP25 elicit a deficit of ATP9 mRNA and of its translation product, thereby preventing assembly of functional F 0 . Unlike Atp9p, the other mitochondrial gene products, including ATPase subunits Atp6p and Atp8p, are synthesized normally in atp25 mutants. Northern analysis of mitochondrial RNAs in an atp25 temperature-sensitive mutant confirmed that Atp25p is required for stability of the ATP9 mRNA. Atp25p is a mitochondrial inner membrane protein with a predicted mass of 70 kDa. The primary translation product of ATP25 is cleaved in vivo after residue 292 to yield a 35-kDa C-terminal polypeptide. The C-terminal half of Atp25p is sufficient to stabilize the ATP9 mRNA and restore synthesis of Atp9p. Growth on respiratory substrates, however, depends on both halves of Atp25p, indicating that the N-terminal half has another function, which we propose to be oligomerization of Atp9p into a proper size ring structure.

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Section: Introductionmentioning

confidence: 99%

ATP25, a New Nuclear Gene ofSaccharomyces cerevisiaeRequired for Expression and Assembly of the Atp9p Subunit of Mitochondrial ATPase

Zeng

Barros

Shul'man

et al. 2008

MBoC

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“…Immediately following early stages of elongation by RNA polymerase, Mtf1p is released from the transcription complex (Shadel and Clayton 1993;Mangus et al 1994;Cliften et al 1997). Processing of the growing polycistronic messengers occurs simultaneously with transcription (Dieckmann and Staples 1994). In the absence of mechanisms for transcription modulation in mitochondria, RNA maturation (e.g., endonucleolytic cleavage, trimming of 5Ј-and 3Ј-ends, splicing) and degradation are key steps in the control of mitochondrial gene expression.…”

Section: Introductionmentioning

confidence: 99%

Transcription and RNA-processing in fission yeast mitochondria

Schäfer¹,

Hansen²,

Lang³

2005

RNA

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We systematically examined transcription and RNA-processing in mitochondria of the petite-negative fission yeast Schizosaccharomyces pombe. Two presumptive transcription initiation sites at opposite positions on the circular-mapping mtDNA were confirmed by in vitro capping of primary transcripts with guanylyl-transferase. The major promoter (P ma ) is located adjacent to the 5-end of the rnl gene, and a second, minor promoter (P mi ) upstream from cox3. The primary 5-termini of the mature rnl and cox3 transcripts remain unmodified. A third predicted accessory transcription initiation site is within the group IIA1 intron of the cob gene (cobI1). The consensus promoter motif of S. pombe closely resembles the nonanucleotide promoter motifs of various yeast mtDNAs. We further characterized all mRNAs and the two ribosomal RNAs by Northern hybridization, and precisely mapped their 5-and 3-ends. The mRNAs have leader sequences with a length of 38 up to 220 nt and, in most instances, are created by removal of tRNAs from large precursor RNAs. Like cox2 and rnl, cox1 and cox3 are not separated by tRNA genes; instead, transcription initiation from the promoters upstream from rnl and cox3 compensates for the lack of tRNA-mediated 5-processing. The 3-termini of mRNAs and of SSU rRNA are processed at distinct, C-rich motifs that are located at a variable distance (1-15 nt) downstream from mRNA and SSU-rRNA coding regions. The accuracy of RNAprocessing at these sites is sequence-dependent. Similar 3-RNA-processing motifs are present in species of the genus Schizosaccharomyces, but not in budding yeasts that have functionally analogous A+T-rich dodecamer processing signals.

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“…10 and 11). In contrast, the mtDNA of the budding yeast Saccharomyces cerevisiae is large (ϳ75-85 kb in size) and has numerous promoters (Ͼ20) that direct different levels of transcription (12)(13)(14). In the fission yeast Schizosaccharomyces pombe, the mtDNA is compact (ϳ19 kb in size) and contains three promoters (15,16).…”

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confidence: 99%

Partitioning of the Nuclear and Mitochondrial tRNA 3′-End Processing Activities between Two different Proteins in Schizosaccharomyces pombe

Zhang

Zhao

Huang

2013

Journal of Biological Chemistry

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Background: Most eukaryotes contain only one tRNase Z gene involved in both nuclear and organellar tRNA 3Ј-end maturation. Results: Schizosaccharomyces pombe has two tRNase Z genes required for nuclear and mitochondrial tRNA 3Ј-end processing, respectively. Conclusion: The evolution of two tRNase Z genes and their differential expression in fission yeast may avoid toxic off-target effects. Significance: The results advance our understanding of tRNA 3Ј-end maturation.

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Regulation of Mitochondrial Gene Expression in Saccharomyces cerevisiae

Cited by 84 publications

References 193 publications

ATP25, a New Nuclear Gene ofSaccharomyces cerevisiaeRequired for Expression and Assembly of the Atp9p Subunit of Mitochondrial ATPase

ATP25, a New Nuclear Gene ofSaccharomyces cerevisiaeRequired for Expression and Assembly of the Atp9p Subunit of Mitochondrial ATPase

Transcription and RNA-processing in fission yeast mitochondria

Partitioning of the Nuclear and Mitochondrial tRNA 3′-End Processing Activities between Two different Proteins in Schizosaccharomyces pombe

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