Regulation of natural-killer cell cytotoxicity and enhancement of complement factors in the spontaneously aborted mouse placenta

Kusakabe, Ken Takeshi; Naka, Mayumi; Ito, Yuko; Eid, Nabil; Otsuki, Yoshinori

doi:10.1016/j.fertnstert.2007.07.1331

Cited by 22 publications

(21 citation statements)

References 35 publications

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“…The alternative complement pathway is usually kept in a weak state of activation at the target cell membrane and the activity is controlled by regulating factors, such as adipsin or factor I. Recently, we have found a significant elevation of adipsin in the mouse placentas from spontaneous abortions [3]. Adipsin, an analogue of human factor D, acts as an enhancer in the alternative pathway, so it has been suggested that aberrant complement activation induced by adipsin is involved with the onset of a spontaneous abortion.…”

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confidence: 99%

“…Absorbed implantation sites were visually identified by the size of shrunken placentas and dissolved and discolored brown embryos. In non-treated pregnant mice, spontaneous abortion occurs at an incidence of 9.96  11.6% at day 14.5 of pregnancy [3]. The experimental protocol using laboratory mice was approved by the Ethical Committee on Animal Experimentation of Osaka Prefecture University, and all mice were treated with humane care.…”

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confidence: 99%

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Regulation of Complement Activity via the Alternative Pathway in Placentas of Mouse Spontaneous Abortions

Takeshita

Nagaishi

Kondo

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. Placental complement has the potential to induce autologous embryo injury. We have previously found a significant elevation of adipsin, an activating factor of the alternative complement pathway, in mouse placentas from spontaneous abortions. The present study was aimed to evaluate regulation of the alternative complement pathway in placentas of mouse spontaneous abortions. Protein was purified from mouse placentas at normal and abortion implantation sites on day 14.5 of pregnancy. The activity of the alternative complement pathway was slightly intensified following addition of protein from abortion placentas. Western blotting revealed that Crry was clearly present in the placentas from abortions. Thus, the complement regulating system through Crry is functional to restrict alternative complement activity in abortion placentas.

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confidence: 99%

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confidence: 99%

Regulation of Complement Activity via the Alternative Pathway in Placentas of Mouse Spontaneous Abortions

Takeshita

Nagaishi

Kondo

et al. 2010

The Journal of Veterinary Medical Science

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“…The results show that 84.3% of implantation sites formed the placenta (average implantation sites at ED9; 10.2 vs average placenta at ED22; 8.6). Several implantation sites would have been lost during midpregnancy in this study because spontaneous abortions occur during mid-pregnancy, even in a normal pregnancy (Kusakabe et al 2008). This is a brand new model investigating the mechanism of spontaneous abortion during mid-pregnancy.…”

Section: Discussionmentioning

confidence: 98%

The complete control of murine pregnancy from embryo implantation to parturition

Terakawa¹,

Watanabe²,

Obara³

et al. 2012

Reproduction

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The ovary is the main secretory source of progestin and estrogen and is indispensable to the maintenance of all events of pregnancy in mice. The purpose of this study was to control all processes of pregnancy in mice, from embryo implantation to parturition, without ovaries. The ovaries were removed before embryo implantation, and a single injection of medroxyprogesterone acetate (MPA) was given. Embryo implantation was induced by leukemia inhibitory factor, which can substitute 17b-estradiol (E 2 ). Continuous exposure to E 2 was necessary at mid-pregnancy, when placentation was completed. All mice sustained pregnancy without ovaries before parturition, which was initiated by the removal of E 2 and MPA. Murine pregnancy is a complicated process involving embryo implantation, placentation, and parturition. Complete control of pregnancy was achieved with the simple treatment of MPA and E 2 after induction of embryo implantation. Here, time-dependent events in the uterus during pregnancy could be realized without the ovaries, because the initiation of each event could be stringently controlled by hormonal treatments.

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“…Also, there is a report that the serum C3 and C4 levels are very high in patients with unexplained recurrent abortions [15]. Trophoblasts in the labyrinthine of abortion placentas change the morphology in post-apoptotic necrosis shapes such as nuclear condensation, rupture of cell membranes and leakage of acid phosphatase-positive lysosomal granules [9]. Adipsin in placentas may bear a role for histological disruption in the process of dissolution and removal of the abortion placentas.…”

Section: Discussionmentioning

confidence: 99%

“…We examined specific gene alterations in mouse pregnant uteri with non-treatment, including approximately 10% spontaneous fetal absorption in total implantation sites, and found a notable increase in adipsin at abortion placentas [9]. Localization of adipsin has been confirmed in adipose tissue, muscle, lungs, and peripheral nerves [10,11], but localization in the placenta has not been previously reported.…”

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confidence: 99%

Elevation of Adipsin, a Complement Activating Factor, in the Mouse Placenta During Spontaneous Abortion

Takeshita

Kondo

Okada

et al. 2010

Journal of Reproduction and Development

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Abstract. The complement system is thought to be precisely regulated during pregnancy. We have examined specific gene profiles in mouse placentas causing spontaneous abortion and found notable up-regulation of adipsin, a complement activating factor. The aim of the present study was to determine the basic kinetics and localization of adipsin in the placenta and the difference in complement activity between normal placentas and placentas of abortuses. Normal and spontaneously absorbed implantation sites obtained from naturally-mated mouse uteri on days 10.5 and 14.5 of pregnancy were processed for histologic studies and protein purification. Adipsin immunoreaction was detected at the decidua basalis in normal placentas and additionally at the placental labyrinth in the absorbed placentas. The quantity of adipsin was increased in the absorbed placentas compared with the normal placentas. In concert with the increase in adipsin, the amounts of complement component 3 and degradation products were elevated and complemental activity was up-regulated in the absorbed placenta. These findings suggest that local expression of adipsin has a reproductive effect at the feto-maternal interface and possibly plays a role in spontaneous abortion.

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Regulation of natural-killer cell cytotoxicity and enhancement of complement factors in the spontaneously aborted mouse placenta

Cited by 22 publications

References 35 publications

Regulation of Complement Activity via the Alternative Pathway in Placentas of Mouse Spontaneous Abortions

Regulation of Complement Activity via the Alternative Pathway in Placentas of Mouse Spontaneous Abortions

The complete control of murine pregnancy from embryo implantation to parturition

Elevation of Adipsin, a Complement Activating Factor, in the Mouse Placenta During Spontaneous Abortion

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