Regulation of p27<sup>Kip1</sup>by miRNA 221/222 in Glioblastoma

Gillies, Jana; Lorimer, Ian A. J.

doi:10.4161/cc.6.16.4526

Cited by 240 publications

(173 citation statements)

References 19 publications

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“…Confirming the findings of He et al, 21 our results showed that miR146b, but not miR-146a, was overexpressed in papillary carcinoma. Also, similar to previous studies, 10,21,49 our results showed that the expression levels of miR-221 and miR-222 are closely coordinated. As both are clustered on X chromosome, 50 it is likely that they are encoded by a single polycistron, as was previously suggested.…”

Section: Discussionsupporting

confidence: 91%

“…In contrast, although miR-146b, -221, and -222 were all upregulated in papillary thyroid carcinoma, our data suggest that they are regulated differently in cancer. Consistent with this notion, miR-221 and miR-222 have been implicated in multiple tumor types, 10,39,49,[51][52][53] including glioblastoma and leukemia, and miR-146 has only been associated with papillary thyroid carcinoma and melanoma. 8,21,22 In summary, we showed that when compared to normal thyroid tissue, miR-146b, miR-221, and miR-222 were overexpressed in almost all cases of papillary thyroid carcinoma but not in follicular adenoma or hyperplastic thyroid nodules.…”

Section: Discussionmentioning

confidence: 71%

“…By using array-based technology and reverse transcription (RT)-PCR, the global miRNA expression profiles as well as the level of individual miRNA species have been analyzed in various human cancers, and aberrant miRNA expression was found in all tumor types studied to date, including hematological malignancies, 6 lymphoma, 7 melanoma, 8 glioblastoma, 9,10 as well as epithelial cancers from lung, 11,12 breast, 13,14 colon, 15,16 liver, 17,18 thyroid, [19][20][21][22] pancreas [23][24][25][26] etc. When compared to their normal-tissue counterparts, both overexpression and underexpression of miRNA has been observed, with decreased expression appearing to be more common in some studies, 8,27 leading to the hypothesis that oncogenes might be target genes for these miRNAs.…”

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confidence: 99%

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MicroRNA analysis as a potential diagnostic tool for papillary thyroid carcinoma

et al. 2008

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MicroRNA (miRNA) microarray analysis has consistently found altered expression of miRNAs in thyroid tumors, suggesting their roles in thyroid carcinogenesis. To explore whether this differential expression can be used as a diagnostic tool in surgical pathology and fine-needle aspirate (FNA) specimens, the expression of selected miRNA was evaluated by quantitative RT-PCR, using total RNA from 84 formalin-fixed paraffin-embedded tissues and 40 ex vivo aspirate specimens. miRNA from all paraffin-embedded tissues and all but one FNA sample were found to be analyzable, with paraffin sections yielding better miRNA quality. Preliminary analysis of 6 miRNAs in 10 papillary thyroid carcinoma and 10 follicular adenoma identified significant overexpression of miR-146b, -221, and -222 in papillary thyroid carcinoma (Po0.02), but not miR-146a, -155, or -187 (P40.08). The expression of these first three miRNAs was examined in a series of 5 normal thyroid, 11 hyperplastic nodules, 24 follicular adenoma, 27 classical papillary thyroid carcinoma, 5 follicular variant papillary thyroid carcinoma, 2 follicular carcinoma, and 10 encapsulated follicular lesions with partial nuclear features of papillary carcinoma. Results showed miR-146b to be most consistently overexpressed in both classical papillary carcinoma and follicular variants, whereas all other groups showed lower expression at a similar level (Po0.001 for pair-wise comparisons between papillary carcinoma and all other groups). Follicular lesions with partial features of papillary carcinoma all showed low miR-146b levels similar to other non-papillary carcinoma groups, suggesting that they are biologically distinctive from papillary carcinoma. miR-221 and miR-222 also showed higher expression in papillary carcinoma, but with substantial overlaps with the other groups. When applied to 40 FNA samples of various lesions, only miR-146b and miR-222 persisted as distinguishing markers for papillary carcinoma. We concluded that miRNAs, particularly miR-146b, might potentially be adjunct markers for diagnosing papillary thyroid carcinoma in both FNA and surgical pathology specimens.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 71%

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confidence: 99%

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MicroRNA analysis as a potential diagnostic tool for papillary thyroid carcinoma

et al. 2008

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“…A recent analysis of the methylation signature in PCa cell lines demonstrated that indeed histone methylation leads to the silencing of the entire mir-22l/222 cluster (Ke et al, 2009). Deregulation of several of the downregulated miRNAs identified in our prognostic profile has been demonstrated also in other human malignancies or diseases such as breast ) and colorectal cancer (Michael et al, 2003) for the mir-143/145 cluster; breast cancer (Zhao et al, 2008) and glioblastoma (Gillies and Lorimer, 2007) for the mir-221/222 cluster; and liver cancer (Datta et al, 2008) or cardiac hypertrophy (Care et al, 2007) for the mir-1/ 133 cluster.…”

Section: Discussionsupporting

confidence: 53%

Diagnostic and prognostic signatures from the small non-coding RNA transcriptome in prostate cancer

et al. 2011

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Prostate cancer (PCa) is the most frequent male malignancy and the second most common cause of cancer-related death in Western countries. Current clinical and pathological methods are limited in the prediction of postoperative outcome. It is becoming increasingly evident that small non-coding RNA (ncRNA) species are associated with the development and progression of this malignancy. To assess the diversity and abundance of small ncRNAs in PCa, we analyzed the composition of the entire small transcriptome by Illumina/ Solexa deep sequencing. We further analyzed the micro-RNA (miRNA) expression signatures of 102 fresh-frozen patient samples during PCa progression by miRNA microarrays. Both platforms were cross-validated by quantitative reverse transcriptase-PCR. Besides the altered expression of several miRNAs, our deep sequencing analyses revealed strong differential expression of small nucleolar RNAs (snoRNAs) and transfer RNAs (tRNAs). From microarray analysis, we derived a miRNA diagnostic classifier that accurately distinguishes normal from cancer samples. Furthermore, we were able to construct a PCa prognostic predictor that independently forecasts postoperative outcome. Importantly, the majority of miRNAs included in the predictor also exhibit high sequence counts and concordant differential expression in Illumina PCa samples, supported by quantitative reverse transcriptase-PCR. Our findings provide miRNA expression signatures that may serve as an accurate tool for the diagnosis and prognosis of PCa.

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“…During the analysis of a global expression level of 245 miRNAs in glioblastoma multiforme, Ciafre et al (2005) observed that miR-221 was highly upregulated. In a recent work, Gillies and Lorimer (2007) have reported that miR-221 and miR-222 directly target p27 Kip1 , a key negative regulator of the cell cycle, in glioblastoma. In another study, Chan et al (2005) also observed that miR-21 was strongly overexpressed (5-to 100-fold) in highly malignant glioblastoma tumor tissues.…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs as potential cancer therapeutics

2008

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Regulation of p27^Kip1by miRNA 221/222 in Glioblastoma

Cited by 240 publications

References 19 publications

MicroRNA analysis as a potential diagnostic tool for papillary thyroid carcinoma

MicroRNA analysis as a potential diagnostic tool for papillary thyroid carcinoma

Diagnostic and prognostic signatures from the small non-coding RNA transcriptome in prostate cancer

MicroRNAs as potential cancer therapeutics

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Regulation of p27Kip1by miRNA 221/222 in Glioblastoma

Cited by 240 publications

References 19 publications

MicroRNA analysis as a potential diagnostic tool for papillary thyroid carcinoma

MicroRNA analysis as a potential diagnostic tool for papillary thyroid carcinoma

Diagnostic and prognostic signatures from the small non-coding RNA transcriptome in prostate cancer

MicroRNAs as potential cancer therapeutics

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Regulation of p27^Kip1by miRNA 221/222 in Glioblastoma