Regulation of photosynthesis in leaves of C4 plants following a transition from high to low light

Doncaster, Helen D.; Adcock, Michael D.; Leegood, Richard C.

doi:10.1016/s0005-2728(89)80419-0

Cited by 23 publications

(21 citation statements)

References 42 publications

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“…The light intensity at the leaf surface was set to 850 pmol m-'s-'. Air jets of 1000 pmol mol-I CO, and 2% O, in N, were directed to both leaf surfaces and after 40 min, when steady-state photosynthesis was reached, the leaf discs were freeze-clamped, which cooled the leaf below 0°C in less than 0.1 s, as described by Doncaster et al (1989).…”

Section: Gas Exchange and Freezing Of Leaf Discsmentioning

confidence: 99%

Effect of High Temperature on Photosynthesis in Beans (II. CO2 Assimilation and Metabolite Contents)

Pastenes

Horton

1996

Plant Physiology

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The effect of high temperatures on CO, assimilation, metabolite content, and capacity for reducing power production in nonphotorespiratory conditions has been assessed in two different bean (Phaseolus vulgarus 1.) varieties, Blue Lake (commercially available i n the United Kingdom) and Barbucho (a noncommercially bred Chilean variety), which are known to differ in their resistance to extreme high temperatures. Barbucho maintains its photosynthetic functions for a longer period of time under extreme heat compared with Blue Lake. The CO, assimilation rate was increased by increases in temperature, with a decrease in ratio of rates at temperatures differing by 10°C. It is suggested that limitations to CO, assimilation are caused by metabolic restrictions that can be differentiated between those occurring in the range of 20 to 30°C and 30 to 35°C. It is likely that changes i n the capacity for Calvin cycle regeneration and starch synthesis affect photosynthesis in the range of 20 to 30°C. But following an increase in temperature from 30 to 35"C, the supply of reducing power becomes limiting. From analysis of adenylate concentration, transthylakoid energization, and, indirectly, NADPH/NADP+ ratio, it was concluded that the limitation in the assimilatory power was due to an oxidation of the NADPH/ NADP+ pool. In the range of 30 to 35"C, the photosystem I quantum yield increased and photosystem II maintained its value. We conclude that the reorganization of thylakoids observed at 30 to 35°C increased the excitation of photosystem I, inducing an increase in cyclic electron transport and a decrease in the supply of NADPH, limiting carbon assimilation.High temperatures affect photosynthesis by altering the excitation energy distribution by changing the structure of thylakoids (Berry and Bjorkman, 1980;Weis and Berry, 1988) and by changing the activity of the Calvin cycle and other metabolic processes such as photorespiration and product synthesis. Much of the attention has been focused on the former aspect, since thylakoids are highly sensitive to heat, whereas the restrictions imposed by high temperatures on carbon metabolism have been nearly exclusively interpreted as the effect on CO, ' Supported by a scholarship to C.P. from the Ministry of Planning of the Chilean Government.Present address: Facultad de Ciencias Agrarias y Forestales, Departamento de Producción Agrícola, Universidad de Chile, Casilla 1004, Santiago, Chile.* Corresponding author; e-mail cpasteneQabello.dic.uchi1e.cl; fax 562-678-5700.availability. The diffusion of CO, and O, and the affinity for carboxylation of the Rubisco enzyme have been proven to be affected by increasing temperatures (Hall and Keys, 1983;Jordan and Ogren, 1984; Brooks and Farquhar, 1985), but it has been argued that the stimulation of photorespiration cannot fully explain the effect of high temperature. It has been suggested that a restricted electron transport may limit RuBP supply (Berry and Bjorkman, 1980).It also has to be considered that changes in temperature induce variati...

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Section: Gas Exchange and Freezing Of Leaf Discsmentioning

confidence: 99%

Effect of High Temperature on Photosynthesis in Beans (II. CO2 Assimilation and Metabolite Contents)

Pastenes

Horton

1996

Plant Physiology

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“…Metabolites were determined spectrophotometrically using an assay buffer containing 0.2 M Tris-HCl (pH 8.1) and 10 mM MgC12. In addition, the reaction mixtures contained: RuBP and 3-phosphoglycerate (PGA) (Doncaster et al 1989), 20 gL extract, 10 mM NaHCO 3, 1 mM ATP, 0.8 mM NADH, 215 nkat glyceraldehyde-3-phosphate dehydrogenase, 250 nkat 3-phosphoglycerate kinase and 5 nkat Rubisco; triose-phosphate (Stitt et al 1989), 50 gL extract, 0.8 mM NADH, 4 nkat glycerol-3-phosphate dehydrogenase, 100/4 nkat triose phosphate isomerase/glycerol-3-phosphate dehydrogenase and 3 nkat aldolase; hexose-phosphate (Stitt et al 1989), 30 p.L extract, 0.5 mM NADP § 13 nkat glucose-6-phosphate dehydrogenase, 60 nkat phosphoglucose isomerase, 13 nkat phosphoglucomutase, 0.8mM pyrophosphate, 80 nkat uridine-5'-diphosphoglucose pyrophosphorylase.…”

Section: Plantmentioning

confidence: 99%

Cold-hardening results in increased activity of enzymes involved in carbon metabolism in leaves of winter rye (Secale cereale L.)

Hurry

Keerberg

Pärnik

et al. 1995

Planta

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Abstract. Light-and CO2-saturated photosynthesis of nonhardened rye (Secale cereale L. cv. Musketeer) was reduced from 18.10 to 7.17 lamol O2-m 2.s i when leaves were transferred from 20 to 5~ for 30 min. Following cold-hardening at 5~ for ten weeks, photosynthesis recovered to 15.05 ~tmol O2.m 2.s 1, comparable to the nonhardened rate at 20~ Recovery of photosynthesis was associated with increases in the total activity and activation of enzymes of the photosynthetic carbon-reduction cycle and of sucrose synthesis. The total hexose-phosphate pool increase by 30% and 120% for nonhardened and cold-hardened leaves respectively when measured at 5~ The large increase in esterified phosphate in coldhardened leaves occurred without a limitation in inorganic phosphate supply. In contrast, the much smaller increase in esterified phosphate in nonhardened leaves was associated with an inhibition of ribulose-l,5-bisphosphate carboxylase/oxygenase and sucrose-phosphate synthase activation. It is suggested that the large increases in hexose phosphates in cold-hardened leaves compensates for the higher substrate threshold concentrations needed for enzyme activation at low temperatures. High substrate concentrations could also compensate for the kinetic limitations imposed by product inhibition from the accumulation of sucrose at 5~ Nonhardened leaves appear to be unable to compensate in this fashion due to an inadequate supply of inorganic phosphate. Fru 1,6BP = fructose-1,6-bisphosphate; Fru 1,6BPase = fructose-1,6-bisphosphatase; Glc6P = glucose-6-phosphate; PGA=3-phosphoglycerate; PPFD=photosynthetic photon flux density; CH=cold-hardened rye grown at 5~ NH = nonhardened rye grown at 24~ Rubisco = ribulose-1,5-bisphosphate carboxylase/oxygenase; RuBP=ribulose-l,5-bisphosphate; SPS=sucrose-phosphate synthase; UDPGlc=uridine 5'-diphosphoglucose Correspondence to: V.M. Hurry; FAX: 61 (6) 2475896

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“…The amount of phaeophytin was determined by the method of Vernon (31). The amounts of RuBP and glycerate-3-P were measured according to Doncaster et al (6). Triose-P were determined spectrophotometrically in an assay solution containing 50 mm Hepes (pH 7.5), 1 mM NaH2AsO4, 1 mM EDTA, 1 mm NAD+, and 10 units each of triose-P isomerase and glyceraldehyde-P dehydrogenase.…”

Section: Metabolite Analysismentioning

confidence: 99%

Changes in Activities of Enzymes of Carbon Metabolism in Leaves during Exposure of Plants to Low Temperature

Holaday¹,

Martindale²,

Alred³

et al. 1992

Plant Physiol.

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255

168

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The aim of this study was to determine the response of photosynthetic carbon metabolism in spinach and bean to low temperature. (a) Exposure of warm-grown spinach and bean plants to 10°C for 10 days resulted in increases in the total activities of a number of enzymes, including ribulose 1,5-bisphosphate carboxylase (Rubisco), stromal fructose 1,6 bisphosphatase (Fru 1,6-P2ase), sedoheptulose 1,7-bisphosphatase (Sed 1,7-P2ase), and the cytosolic Fru 1,6-P2ase. In spinach, but not bean, there was an increase in the total activity of sucrose-phosphate synthase. (b) The C02-saturated rates of photosynthesis for the coldacclimated spinach plants were 68% greater at 100C than those for warm-acclimated plants, whereas in bean, rates of photosynthesis at 10°C were very low after exposure to low temperature. (c) When spinach leaf discs were transferred from 27 to 100C, the stromal Fru 1,6-P2ase and NADP-malate dehydrogenase were almost fully activated within 8 minutes, and Rubisco reached 90% of full activation within 15 minutes of transfer. An initial restriction of Calvin cycle fluxes was evident as an increase in the amounts of ribulose 1,5-bisphosphate, glycerate-3-phosphate, Fru 1,6-P2, and Sed 1,7-P2. In bean, activation of stromal Fru 1,6-P2ase was weak, whereas the activation state of Rubisco decreased during the first few minutes after transfer to low temperature. However, NADP-malate dehydrogenase became almost fully activated, showing that no loss of the capacity for reductive activation occurred. (d) Temperature compensation in spinach evidently involves increases in the capacities of a range of enzymes, achieved in the short term by an increase in activation state, whereas long-term acclimation is achieved by an increase in the maximum activities of enzymes. The inability of bean to activate fully certain Calvin cycle enzymes and sucrose-phosphate synthase, or to increase nonphotochemical quenching of chlorophyll fluorescence at 100C, may be factors contributing to its poor performance at low temperature. complete in evergreen woody species that are subject to large seasonal variations in temperature, such as Eucalyptus species and the desert evergreen, Nerium oleander. For such plants acclimated to low temperature, temperature response curves for photosynthesis indicate an increased photosynthetic capacity over a wide range of temperatures (2, 7). The increases in photosynthetic capacity that result from acclimation to a lower growth temperature could be the result of a number of factors, as plants acclimating to low temperature show increases in, for example, soluble protein, the rate of electron transport, and in the activities of enzymes such as Rubisco and the stromal Fru 1,6-P2ase,2 which parallel the increase in photosynthetic capacity (2, 3).There are a number of other reports of increases in Rubisco at lower temperatures, for example, in the arctic-alpine species Oxyria digyna (5), in the C4 plant Atriplex lentiformis (24), and in the grass Dactylis glomerata (30). Gas-exchange studies also...

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Regulation of photosynthesis in leaves of C4 plants following a transition from high to low light

Cited by 23 publications

References 42 publications

Effect of High Temperature on Photosynthesis in Beans (II. CO2 Assimilation and Metabolite Contents)

Effect of High Temperature on Photosynthesis in Beans (II. CO2 Assimilation and Metabolite Contents)

Cold-hardening results in increased activity of enzymes involved in carbon metabolism in leaves of winter rye (Secale cereale L.)

Changes in Activities of Enzymes of Carbon Metabolism in Leaves during Exposure of Plants to Low Temperature

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