Regulation of Plant NADPH Oxidase

Abstract

The production of Reactive Oxygen Species (ROS) is one of the key events occurring during the response of plants to environmental changes, and contributing to establish adaptive signaling pathways. A plasma membrane bound NADPH oxidase enzyme has been evidenced as the ROS producing system in various plant-microorganisms interactions. We very recently reported, that a protein of the 14-3-3 family was able to interact directly with the C-terminus part of this NADPH oxidase, and that modification of its expression in tobacco cells led to reduced amount of ROS production upon elicitation. In this addendum, we summarize this work, present additional results, and propose an hypothetic model of regulation of this oxidase in a plant defense context.

Hydrogen cyanamide breaks grapevine bud dormancy in the summer through transient activation of gene expression and accumulation of reactive oxygen and nitrogen species

“… [ 26 ] 8. NADPH oxidase A plasma membrane NADPH oxidase produces ROS in planta or in elicited cells during incompatible interaction [ 100 ] 9. PR proteins The class 1 pathogenesis-related (PR) proteins are thought to be involved in plant defense responses against infection by pathogens, such as fungi or viruses.…”

Transcriptome profile of Carrizo citrange roots in response toPhytophthora parasiticainfection

“…Differential proteome analysis of a resistant soybean cultivar in response to P. sojae also revealed significant upregulation in oxidoreductase activity related proteins (Qiu et al 2009). A tobacco NADPH oxidase was found responsible for triggering HR in response to a Phytophthora cryptogea elicitor leading to acquired resistance (Elmayan and Simon-Plas 2007). An NADPH oxidase (TRINITY_DN6244_c1/c0) was 512 times more expressed in citrus roots infected with P. parasitica compared to control (Appendix S2) so it might be involved in ROS signaling during early stages of infection but that was not enough to prevent pathogen colonization.…”

“…Unlike susceptible A. thaliana roots where number of DEGs didn't increase considerably with time during P. parasitica infection (Le Berre et al 2017), total number of DETs increased in citrus roots from 24 to 48 hpi suggesting induction of stronger defense response towards P. parasitica overtime that can be related to tolerance response during later stages (Figure 7 and Figure S4). Upon perception of pathogen generation of reactive oxygen species (ROS) has been reported as one of the earliest defense strategies in plants (Elmayan and Simon-Plas 2007). Oxidoreductase activity was found to be the most significantly differentially regulated MF in roots upon P. parasitica infection and several different oxidases and reductases were found to be highly upregulated.…”

Common ragweed (Ambrosia artemisiifolia L.): allergenicity and molecular characterization of pollen after plant exposure to elevated NO2

“…), and they play a key role in pollen tube elongation. Interestingly, a 14‐3‐3 protein was shown to interact with NADPH oxidase, thus influencing reactive oxygen species (ROS) production (Elmayan & Simon‐Plas ). As the NADPH oxidase/ROS system influences the allergenicity of pollen (Wang et al .…”