Regulation of preadipocyte factor-1 gene expression during 3T3-L1 cell differentiation.

Boney, Charlotte M.; Fiedorek, Frederick T.; Paul, Sofia; Gruppuso, Philip A.

doi:10.1210/endo.137.7.8770915

Cited by 36 publications

(29 citation statements)

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“…Tumor necrosis factor α, interferon γ, and interleukins 1 and 11 strongly decrease adipose conversion in cell lines as well as in rat, porcine and human primary cells [22,27,56,91,133,178,240,247]. The mechanism of action of TNFα has been largely investigated.…”

Section: Other Growth Factors and Cytokinesmentioning

confidence: 99%

The adipose conversion process: regulation by extracellular and intracellular factors

Boone

Mourot

Gregoire³

et al. 2000

Reprod. Nutr. Dev.

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-White adipose tissue regulates lipid metabolism and acts as a secretory organ. Because of its importance for human health and animal production, many studies have attempted to better understand its development at the cellular and molecular levels by culturing preadipose cells in vitro. This synthesis article describes our current knowledge, acquired by this approach, concerning the regulation of the different steps of the adipocyte differentiation program by extracellular (hormones, cytokines, growth factors, retinoids and fatty acids) and intracellular agents (second messengers and transcription factors). The discrepant effects that have been observed for some of these factors are also discussed. This information is very important in the perspective of a better control of fat deposits in human and breeding species.preadipocyte / differentiation / hormonal agent / second messenger / transcription factor Résumé -Le processus d'adipoconversion : sa régulation par des facteurs extracellulaires et intracellulaires. Le tissu adipeux blanc régule le métabolisme lipidique et agit comme un organe de sécrétion. Étant donné son importance pour la santé humaine et la production animale, de nombreuses études ont tenté de mieux comprendre son développement aux niveaux cellulaire et moléculaire en utilisant des cultures de préadipocytes. Cet article de synthèse décrit notre connaissance actuelle, issue de cette approche, concernant la régulation des différentes étapes du programme de la diffé-renciation adipocytaire par des facteurs extracellulaires (hormones, cytokines, facteurs de croissance, rétinoïdes et acides gras) et intracellulaires (seconds messagers et facteurs de transcription). Les effets divergents observés pour certains de ces facteurs sont également discutés. Ces informations sont très importantes dans la perspective d'un meilleur contrôle des dépôts adipeux chez l'humain et les espèces d'élevage.préadipocyte / différenciation / hormone / second messager / facteur de transcription Reprod. Nutr. Dev. 40 (2000) 325-358 325

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Section: Other Growth Factors and Cytokinesmentioning

confidence: 99%

The adipose conversion process: regulation by extracellular and intracellular factors

Boone

Mourot

Gregoire³

et al. 2000

Reprod. Nutr. Dev.

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“…The catabolic and antiadipogenic effects of TNFa decrease adipose conversion in various cell lines as well as in rat, porcine, and human primary cells (Grégoire et al 1992, Hotamisligil et al 1994, Vassaux et al 1994, Boney et al 1996, Boone et al 2000. TNFa acts directly on lipid metabolism by decreasing parameters related with fatty acid uptake (decrease in lipoprotein lipase (LPL), fatty acid transport (FAT), and fatty acid transport protein (FATP) expressions) and lipogenesis (decrease in fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC) expression, and glycerol-3-phosphate dehydrogenase (GPDH) activity), and by increasing lipolysis (Dani et al 1989, Sethi & Hotamisligil 1999.…”

Section: Introductionmentioning

confidence: 99%

Regulation of proliferation and differentiation of adipocyte precursor cells in rainbow trout (Oncorhynchus mykiss)

Bouraoui¹,

Gutiérrez²,

Navarro³

2008

Journal of Endocrinology

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Here, we describe optimal conditions for the culture of rainbow trout (Oncorhynchus mykiss) pre-adipocytes obtained from adipose tissue and their differentiation into mature adipocytes, in order to study the endocrine control of adipogenesis. Pre-adipocytes were isolated by collagenase digestion and cultured on laminin or 1% gelatin substrate. The expression of proliferating cell nuclear antigen was used as a marker of cell proliferation on various days of culture. Insulin growth factor-I stimulated cell proliferation especially on days 5 and 7 of culture. Tumor necrosis factor a (TNFa) slightly enhanced cell proliferation only at a low dose. We verified the differentiation of cells grown in specific medium into mature adipocytes by oil red O (ORO) staining. Quantification of ORO showed an increase in triglycerides throughout culture.Immunofluorescence staining of cells at day 11 revealed the expression of CCAAT/enhancer-binding protein and peroxisome proliferator-activator receptor g, suggesting that these transcriptional factors are involved in adipocyte differentiation in trout. We also examined the effect of TNFa on the differentiation of these adipocytes in primary culture. TNFa inhibited the differentiation of these cells, as indicated by a decrease in glycerol-3-phosphate dehydrogenase activity, an established marker of adipocyte differentiation. In conclusion, the culture system described here for trout pre-adipocytes is a powerful tool to study the endocrine regulation of adipogenesis in this species.

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“…It is well established that IGF-I mitogenic effects are mediated, at least in part, by the members of the mitogen-activated protein kinase family, termed extracellular-signal-regulated kinase 1 and 2 (ERK1 and ERK2), as well as by the activation of phosphoinositide 3-kinase (PI 3-kinase) and Akt. The mechanisms by which IGF-I promotes the differentiation of various mesenchymal cell types, including adipocytes and cardiomyocytes (Boney et al 1996, Milasincic et al 1996, Boney et al 1998, are also under investigation. All of these cell types have in common a requirement that cell-cycle arrest occur prior to progression to differentiation.…”

Section: Introductionmentioning

confidence: 99%

Insulin-like growth factor-I signaling is modified during chondrocyte differentiation

Phornphutkul¹,

Wu²,

Xu³

et al. 2004

Journal of Endocrinology

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Insulin-like growth factor-I (IGF-I) is a critical regulator of skeletal growth. While IGF-I has been shown to be a potent chondrocyte mitogen in vitro, its role in chondrocyte differentiation is less well characterized. We chose to study the action of IGF-I on an accepted model of chondrocyte differentiation, the ATDC5 cell line. Insulin concentrations sufficiently high to interact with the IGF-I receptor are routinely used to induce ATDC5 cells to differentiate. Therefore, we first examined the ability of IGF-I to promote chondrocyte differentiation at physiological concentrations. IGF-I could induce differentiation of these cells at concentrations below 10 nM. However, increasing IGF-I concentrations were less potent at inducing differentiation. We hypothesized that mitogenic effects of IGF-I might inhibit its differentiating effects. Indeed, the extracellular-signal-regulated kinase (ERK)-pathway inhibitor PD98059 inhibited ATDC5 cell DNA synthesis while enhancing differentiation. This suggested that the ability of IGF-I to promote both proliferation and differentiation might require that its signaling be modulated through the differentiation process. We therefore compared IGF-I-mediated ERK activation in proliferating and hypertrophic chondrocytes. IGF-I potently induced ERK activation in proliferating cells, but minimal ERK response was seen in hypertrophic cells. In contrast, IGF-I-mediated Akt activation was unchanged by differentiation, indicating intact upstream IGF-I receptor signaling. Similar findings were observed in the RCJ3·1C5·18 chondrogenic cell line and in primary chick chondrocytes. We conclude that IGF-I promotes both proliferation and differentiation of chondrocytes and that the differentiation effects of IGF-I may require uncoupling of signaling to the ERK pathway.

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Regulation of preadipocyte factor-1 gene expression during 3T3-L1 cell differentiation.

Cited by 36 publications

References 0 publications

The adipose conversion process: regulation by extracellular and intracellular factors

The adipose conversion process: regulation by extracellular and intracellular factors

Regulation of proliferation and differentiation of adipocyte precursor cells in rainbow trout (Oncorhynchus mykiss)

Insulin-like growth factor-I signaling is modified during chondrocyte differentiation

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