Regulation of proliferation of peritoneal tissue repair cells by peritoneal macrophages

Fukasawa, Manabu; Campeau, Joseph D.; Yanagihara, Donna L.; Rodgers, Kathleen E.; diZerega, Gere S.

doi:10.1016/0022-4804(90)90114-h

Cited by 20 publications

(10 citation statements)

References 28 publications

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“…In addition, bFGF has been shown to intensify multiplication of human peritoneal fibroblasts, with a much stronger impact than TGF-β1. In their studies, Fukasawa et al and Beavis et al stated that recombinant human FGF-basic led to a considerable increase in the reproduction of peritoneal fibroblasts (44,45). In our study, we found no intergroup differences in terms of serum and dialysate bFGF levels, nor did we detect any effect of MMF and RAP on bFGF secretion.…”

Section: Türk Nefroloji Diyaliz Ve Transplantasyon Dergisi Turkish Necontrasting

confidence: 56%

Effects of Mycophenolate Mofetil and Rapamycin on Peritoneal Fibrosis in an Experimental Model of Peritoneal Dialysis

Dursun¹,

Noyan²,

Erdoğan³

et al. 2015

Turk Neph Dial Transpl

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OBJEcTIVE: This study aimed to investigate the effect of mycophenolate mofetil and rapamycin on peritoneal function and morphology in a rat model of experimental peritoneal dialysis. MATERIAL and METHODS:A total of 60 male Albino rats (Wistar strain) were equally divided into four groups as follows: Mycophenolate mofetil group (MMFG), rapamycin group (RAPG) and no-treatment group (NTG) receiving 15 mL 3.86% glucose solution, and control group (CG) receiving daily intraperitoneal injection of 15 mL normal saline. Mycophenolate mofetil and rapamycin were administered orally via daily gastric gavage in MMFG and RAPG. We conducted one-hour peritoneal equilibration tests at 8 weeks, measuring ultrafiltration, serum and dialysate growth factors and cytokines. Peritoneal membrane morphology was examined under the light microscope. RESULTS:Compared to controls, levels of serum and dialysate vascular endothelial growth factor were significantly higher in NTG (p<0.05), but they did not differ significantly in MMFG and RAPG (p>0.05). Net ultrafiltration was significantly higher in MMFG than in NTG (p<0.05). A significant decline was detected in the number of mesothelial, inflammatory and fibroblast cells and peritoneal membrane thickness in MMFG, as compared with NTG. cONcLUSION:We conclude that peritoneal functions such as ultrafiltration capacity can be protected by treatment with MMF and RAP, as they produce a membrane-protective effect in peritoneal dialysis. KEY wORDS:Experimental peritoneal dialysis, Mycophenolate mofetil, Peritoneal fibrosis, Rapamycin, Rat ÖZ AMAÇ: Çalışmanın amacı deneysel bir sıçan periton diyaliz modelinde mikofenolat mofetil ve rapamisinin periton fonksiyonu ve morfolojisi üzerine etkisini araştırmaktır. GEREÇ ve YÖNTEMLER:Wistar-Albino 60 sıçan 4 eşit gruba ayrıldı: Mikofenolat mofetil grubu (MMFG), rapamisin grubu (RAPG) ve tedavi almayan grubu (NTG) %3,86 glikozlu solüsyonundan 15 mL ve kontrol grubu (CG) normal izotonik sıvıdan 15 mL günde tek doz intraperitoneal enjeksiyonla aldı. MMFG ve RAPG günlük gastrik gavajla sırasıyla mikofenolat mofetil ve rapamisin aldılar. Sekiz hafta sonunda 1 saatlik bir periton eşitleme testi yapıldı. Ultrafiltrasyon, serum ve diyalizatta büyüme faktörleri ve sitokinler ölçüldü. Periton membran morfolojisi ışık mikroskopisi ile değerlendirildi.BULGULAR: Serum ve diyalizat vasküler endotelyal büyüme faktör düzeyleri CG ile karşılaştırıl-dığında NTG da anlamlı oranda daha yüksek saptandı (p<0,05), fakat MMFG ve RAPG gruplarında anlamlı fark saptanmadı (p>0,05). Net ultrafiltrasyon NTG'la karşılaştırıldığında MMFG'da anlamlı oranda daha yüksekti (p<0,05). NTG ile karşılaştırıldığında mezotelyal, inflamatuvar ve fibroblast hücre sayısı ile periton membran kalınlığı MMFG'da anlamlı oranda daha düşük saptandı. SONUÇ:Ultrafiltrasyon gibi periton fonksiyonlarının MMF ve RAP ile korunduğu ve bu ilaçların periton diyalizinde periton membranını koruyucu etkiye sahip oldukları sonucuna vardık.

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Section: Türk Nefroloji Diyaliz Ve Transplantasyon Dergisi Turkish Necontrasting

confidence: 56%

Effects of Mycophenolate Mofetil and Rapamycin on Peritoneal Fibrosis in an Experimental Model of Peritoneal Dialysis

Dursun¹,

Noyan²,

Erdoğan³

et al. 2015

Turk Neph Dial Transpl

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“…Subsequently, many studies have provided additional information about the multifunctional role of macrophages within wounds. For example, macrophages have been shown to affect the extracellular matrix composition after injury via the production of extracellular matrix molecules, proteases, and protease inhibitors (3)(4)(5)(6). Macrophages also affect tissue repair through the production of soluble growth factors that influence the proliferation and differentiation of endothelial cells, fibroblasts, keratinocytes, and other cell types (7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

MIP-1alpha as a critical macrophage chemoattractant in murine wound repair.

DiPietro¹,

Burdick²,

Low³

et al. 1998

J. Clin. Invest.

253

175

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At sites of injury, macrophages secrete growth factors and proteins that promote tissue repair. While this central role of the macrophage has been well studied, the specific stimuli that recruit macrophages into sites of injury are not well understood. This study examines the role of macrophage inflammatory protein 1 ␣ (MIP-1 ␣ ), a C-C chemokine with monocyte chemoattractant capability, in excisional wound repair. Both MIP-1 ␣ mRNA and protein were detectable in murine wounds from 12 h through 5 d after injury. MIP-1 ␣ protein levels peaked 3 d after injury, coinciding with maximum macrophage infiltration. The contribution of MIP-1 ␣ to monocyte recruitment into wounds was assessed by treating mice with neutralizing anti-MIP-1 ␣ antiserum before injury. Wounds of mice treated with anti-MIP-1 ␣ antiserum had significantly fewer macrophages than control (41% decrease, P Ͻ 0.01). This decrease in wound macrophages was paralleled by decreased angiogenic activity and collagen synthesis. When tested in the corneal micropocket assay, wound homogenates from mice treated with anti-MIP-1 ␣ contained significantly less angiogenic activity than control wound homogenates (27% positive for angiogenic activity versus 91% positive in the control group, P Ͻ 0.01). Collagen production was also significantly reduced in the wounds from anti-MIP-1 ␣ treated animals (29% decrease, P Ͻ 0.05). The results demonstrate that MIP-1 ␣ plays a critical role in macrophage recruitment into wounds, and suggest that appropriate tissue repair is dependent upon this recruitment. (

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“…Macrophages obtained from the peritoneal cavity on various days after surgi cal trauma can either increase or decrease the proliferation of fibroblasts [18,19]. Further more, healing-phase macrophages were de tected in the majority of membranes from patients with PVR following rhegmatogenous retinal detachment or trauma, while inflam matory-stage macrophages were found in membranes from patients with a short history of the disease [20], The capacity of RPE cells to support the partial macrophage maturation was also time-dependent.…”

Section: Resultsmentioning

confidence: 97%

Monocyte-Macrophage Differentiation Induced by Coculture of Retinal Pigment Epithelium Cells with Monocytes

Osuský

Aurora²,

Ryan³

1997

Ophthalmic Res

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Macrophages play an important role in proliferative vitreoretinopathy (PVR). Since macrophage maturation may be modulated by the local microenvironment, we determined the effect of retinal pigment epithelium (RPE) cells interacting with monocytes on macrophage maturation. The enriched monocyte fraction of peripheral blood mononuclear cells was cocultured with RPE cells. Cell-free supernatants conditioned during the culture periods 0–4 days (growing RPE cells) and 4–8 days (confluent RPE cells) were tested for their capacity to induce monocyte/macrophage differentiation of the promyelocytic cell line HL-60, which was measured by the expression of CD11c and CD14 and flow cytometry. RPE cells released factors that increased the CD14 expression on HL-60 cells in terms of percentage of positive cells (22.7% vs. control 10.4%). RPE-cell-conditioned supernatants had no effect on the CD11c expression. Monocytes secreted substances that increased the expression of CD11c (20.5% vs. control 9.1%; p = 0.003) and CD14 (31.6% vs. control 10.4%; p < 0.0001). Supernatants from cocultures increased the CD11c (19.8%) and CD14 expression (40.8%) to values that were similar to the sum of those of cell monocultures. Supernatants conditioned during the later culture had no effect on CD14 and CD11c expression. We conclude that invading monocytes and RPE cells could create an intraocular microenvironment that supports macrophage maturation during the initial stage of PVR.

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Regulation of proliferation of peritoneal tissue repair cells by peritoneal macrophages

Cited by 20 publications

References 28 publications

Effects of Mycophenolate Mofetil and Rapamycin on Peritoneal Fibrosis in an Experimental Model of Peritoneal Dialysis

Effects of Mycophenolate Mofetil and Rapamycin on Peritoneal Fibrosis in an Experimental Model of Peritoneal Dialysis

MIP-1alpha as a critical macrophage chemoattractant in murine wound repair.

Monocyte-Macrophage Differentiation Induced by Coculture of Retinal Pigment Epithelium Cells with Monocytes

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Regulation of proliferation of peritoneal tissue repair cells by peritoneal macrophages

Cited by 20 publications

References 28 publications

Effects of Mycophenolate Mofetil and Rapamycin on Peritoneal Fibrosis ￼in an Experimental Model of Peritoneal Dialysis

Effects of Mycophenolate Mofetil and Rapamycin on Peritoneal Fibrosis ￼in an Experimental Model of Peritoneal Dialysis

MIP-1alpha as a critical macrophage chemoattractant in murine wound repair.

Monocyte-Macrophage Differentiation Induced by Coculture of Retinal Pigment Epithelium Cells with Monocytes

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Product

Resources

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Effects of Mycophenolate Mofetil and Rapamycin on Peritoneal Fibrosis in an Experimental Model of Peritoneal Dialysis

Effects of Mycophenolate Mofetil and Rapamycin on Peritoneal Fibrosis in an Experimental Model of Peritoneal Dialysis