Regulation of protein corona on liposomes using albumin-binding peptide for targeted tumor therapy

Li, Hanmei; Dai, Yu‐Tzu; Liao, Jiaying; Wang, Yao; Gou, Rui; Tang, Chuane; Li, Wei; Liu, Yi; Fu, Jiao; Shi, Sanjun; Zou, Liang

doi:10.1016/j.jconrel.2023.02.004

Cited by 31 publications

(16 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Emphasis should be placed on the significance of investigating BSA adsorption on liposomes, especially since albumin precoating has become an attractive strategy for prolonging nanocarrier plasma half-life, , as well as improving the release kinetics of liposomal carriers . In particular, liposomes decorated with a 46-residue albumin binding domain were recently shown to specifically bind endogenous albumin, which competitively inhibited opsonin adsorption and prolonged liposomal in vivo circulation . At the same time, as albumin is a carrier protein that possesses ligand binding pockets for many anticancer drugs, it could be used for drug or prodrug complexation prior to precoating to increase drug half-life and improve targeted delivery .…”

Section: Resultsmentioning

confidence: 99%

Impact of Lipid Composition on Vesicle Protein Adsorption: A BSA Case Study

Amărandi,

Neamṭu,

Ştiufiuc

et al. 2024

ACS Omega

View full text Add to dashboard Cite

Investigating the interaction between liposomes and proteins is of paramount importance in the development of liposomal formulations with real potential for bench-to-bedside transfer. Upon entering the body, proteins are immediately adsorbed on the liposomal surface, changing the nanovehicles' biological identity, which has a significant impact on their biodistribution and pharmacokinetics and ultimately on their therapeutic effect. Albumin is the most abundant plasma protein and thus usually adsorbs immediately on the liposomal surface. We herein report a comprehensive investigation on the adsorption of model protein bovine serum albumin (BSA) onto liposomal vesicles containing the zwitterionic lipid 1,2-dipalmitoyl-sn-glycero-3phosphocholine (DPPC), in combination with either cholesterol (CHOL) or the cationic lipid 1,2-dioleoyl-3-trimethylammoniumpropane (DOTAP). While many studies regarding protein adsorption on the surface of liposomes with different compositions have been performed, to the best of our knowledge, the differential responses of CHOL and DOTAP upon albumin adsorption on vesicles have not yet been investigated. UV−vis spectroscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed a strong influence of the phospholipid membrane composition on protein adsorption. Hence, it was found that DOTAP-containing vesicles adsorb proteins more robustly but also aggregate in the presence of BSA, as confirmed by DLS and TEM. Separation of liposome−protein complexes from unadsorbed proteins performed by means of centrifugation and size exclusion chromatography (SEC) was also investigated. Our results show that neither method can be regarded as a golden experimental setup to study the protein corona of liposomes. Yet, SEC proved to be more successful in the separation of unbound proteins, although the amount of lipid loss upon liposome elution was higher than expected. In addition, coarse-grained molecular dynamics simulations were employed to ascertain key membrane parameters, such as the membrane thickness and area per lipid. Overall, this study highlights the importance of surface charge and membrane fluidity in influencing the extent of protein adsorption. We hope that our investigation will be a valuable contribution to better understanding protein−vesicle interactions for improved nanocarrier design.

show abstract

Section: Resultsmentioning

confidence: 99%

Impact of Lipid Composition on Vesicle Protein Adsorption: A BSA Case Study

Amărandi,

Neamṭu,

Ştiufiuc

et al. 2024

ACS Omega

View full text Add to dashboard Cite

show abstract

“…Multivariate statistical analyses, including PCA and OPLS-DA, were used to distinguish differences in the porcine liver lipidome [ 40 ]. A one-way analysis of variance was used for the comparison of lipid species among groups in GraphPad Prism 8.0 [ 41 , 42 ]. DALs between the groups were screened according to a fold change ≤0.5 or ≥2 and variable importance in projection >1.…”

Section: Methodsmentioning

confidence: 99%

Divergence of Liver Lipidomes in Tibetan and Yorkshire Pigs Living at Different Altitudes

Luo

et al. 2023

Molecules

View full text Add to dashboard Cite

The Tibetan pig is a characteristic breed of the Qinghai-Tibet Plateau with distinct physiological and meat quality attributes. The liver lipid profile can offer an important perspective to explore the uniqueness of Tibetan pigs. A quantitative comparison of liver lipidomes revealed significant differences in the lipid profiles between Tibetan and Yorkshire pigs raised at different altitudes. The abundance of lipids in the livers of pigs raised at a high altitude was higher than that of pigs raised at a lower altitude, whereas the abundance of lipids in the livers of Yorkshire pigs was higher than that of Tibetan pigs raised at the same altitude. Of the 1101 lipids identified, 323 and 193 differentially abundant lipids (DALs) were identified in the pairwise comparisons of Tibetan and Yorkshire pigs raised at different altitudes, respectively. The DALs of Tibetan pigs consisted mainly of 161 triglycerides, along with several acylcarnitines, represented by carnitine C2:0, and significant changes in the abundance of some phospholipids. The DALs of Yorkshire pigs were more complex, with significant increases in the abundance of triglycerides, cholesteryl esters, and free fatty acids, and decreases in the abundance of some phospholipids. This research provides strong theoretical and data support for the high-quality development of the highland livestock industry.

show abstract

“…Therefore, modifying nanoparticles with the corona proteins can improve drug delivery. 103 Therefore, enhancing the biocompatibility of nanozymes by surface modification or coating will be an effective measure to improve the safety of nanozymes.…”

Section: Conclusion and Prospectsmentioning

confidence: 99%