Regulation of Rat Bone Sialoprotein Gene Transcription by Enamel Matrix Derivative

Shimizu, Emi; Nakajima, Yu; Kato, Naohiko; Nakayama, Youhei; Saito, Ryoichiro; Samoto, Hiroshi; Ogata, Yorimasa

doi:10.1902/jop.2004.75.2.260

Cited by 45 publications

(45 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although ROS cells originated from rat osteosarcoma, these cells have been treated in numerous studies as typical rat osteoblasts or osteoblast-like cells [16][17][18]. The cells were maintained in a-minimal essential medium (a-MEM; Gibco BRL, Rockville, USA) containing 10% (v/v) heat-inactivated fetal bovine serum (HyClone Laboratories, Logan, USA) and 1% (v/v) penicillin-streptomycin solution (Sigma Chemical, St Louis, USA) at 378C in a humidified atmosphere of 95% air and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Daily low-intensity pulsed ultrasound-mediated osteogenic differentiation in rat osteoblasts

Suzuki¹,

Takayama²,

Suzuki³

et al. 2009

ABBS

View full text Add to dashboard Cite

There were few studies investigating the effects of the mechanical stimulation provided by daily low-intensity pulsed ultrasound (LIPUS) treatment. LIPUS is known to accelerate bone mineralization and regeneration; however, the precise cellular mechanism is unclear. Our purpose was to determine how daily LIPUS treatment affected cell viability, alkaline phosphatase activity, osteogenesis-related gene expression, and mineralized nodule formation in osteoblasts. The typical osteoblastic cell line ROS 17/2.8 cells were cultured in the absence or presence of LIPUS stimulation. Daily LIPUS treatments (1.5 MHz; 20 min) were administered at an intensity of 30 mW/cm 2 for 14 days.Expression of osteogenesis-related genes was examined at mRNA levels using real-time polymerase chain reaction and at protein levels using western blotting analysis. LIPUS stimulation did not affect the rate of cell viability. Alkaline phosphatase activity was increased after 10 days of culture with daily LIPUS stimulation. LIPUS significantly increased the expression of mRNAs encoding Runx2, Msx2, Dlx5, osterix, bone sialoprotein, and bone morphogenetic protein-2, whereas it significantly reduced the expression of mRNA encoding the transcription factor AJ18. Mineralized nodule formation was markedly increased on Day 14 of LIPUS stimulation. LIPUS stimulation directly affected osteogenic cells, leading to mineralized nodule formation. LIPUS is likely to have a fundamental influence on key functional activities of osteoblasts in alveolar bone.

show abstract

Section: Methodsmentioning

confidence: 99%

Daily low-intensity pulsed ultrasound-mediated osteogenic differentiation in rat osteoblasts

Suzuki¹,

Takayama²,

Suzuki³

et al. 2009

ABBS

View full text Add to dashboard Cite

show abstract

“…At the specified time periods, the medium was replaced with fresh medium containing 10 volume fraction % Cell Counting reagent, and the incubation continued for 1 h. After incubation, the absorbance of the reaction products was measured at 450 nm with a microtitre plate reader (Titertec Multiskan Plus, Flow Laboratory, McLean, VA, USA). The cell number was calculated from the absorbance value relative to a standard curve 18) .…”

Section: Determination Of Cell Proliferationmentioning

confidence: 99%

Effects of a calcium phosphate cement on mineralized nodule formation compared with endodontic cements

et al. 2012

View full text Add to dashboard Cite

The aim of this study was to investigate mineralizing ability of a premixed calcium phosphate cement (premixed-CPC) compared to mineral trioxide aggregate (MTA) and zinc oxide eugenol cement (SuperEBA) in ROS17/2.8 cells. The measurements of cell proliferation, alkaline phosphatase (ALPase) activity and mineralized nodule formation in the presence or absence (control) of the test materials were performed using a cell culture insert method with the test materials placed on a porous membrane of culture plate insert. Mineralized nodules were detected by staining with alizarin red, and the calcium content of the mineralized nodules was determined quantitatively using a calcium assay kit. Premixed-CPC and MTA indicated significantly higher cell proliferation, ALPase activity, mineralized nodule formation, and calcium content in nodules than those of SuperEBA (p<0.05). The present results suggest that premixed-CPC has the same mineralizing ability as MTA.

show abstract

“…The effect of up regulation in the ECM formation related genes in the EMD group agrees with other research. 24,25 This indicates that the presence of EMD on PDL cell differentiation is crucial in the initial stages of culture. 26 The relatively low response might be associated with the dosage and application method in our 3D mode, as the effect was not as obvious in 2D culture systems.…”

Section: D Model To Study the Regeneration Of The Pdlmentioning

confidence: 98%

A Three-Dimensional Cell Culture Model to Study the Mechano-Biological Behavior in Periodontal Ligament Regeneration

Oortgiesen

Bronckers

et al. 2012

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

Periodontitis is a disease affecting the supporting structures of the teeth, which can eventually result in tooth loss. A three-dimensional (3D) tissue culture model was developed that may serve to grow a 3D construct that not only transplants into defective periodontal sites, but also allows to examine the effect of mechanical load in vitro. In the current in vitro study, green fluorescent protein labeled periodontal ligament (PDL) cells form rat incisors were embedded in a 3D matrix and exposed to mechanical loading alone, to a chemical stimulus (Emdogain; enamel matrix derivative [EMD]) alone, or a combination of both. Loading consisted of unilateral stretching (8%, 1 Hz) and was applied for 1, 3, or 5 days. Results showed that PDL cells were distributed and randomly oriented within the artificial PDL space in static culture. On mechanical loading, the cells showed higher cell numbers. Moreover, cells realigned perpendicular to the stretching force depending on time and position, with great analogy to natural PDL tissue. EMD application gave a significant effect on growth and upregulated bone sialoprotein (BSP) and collagen type-I (Col-I), whereas Runx-2 was downregulated. This implies that PDL cells under loading might tend to act similar to bone-like cells (BSP and Col-I) but at the same time, react tendon like (Runx-2). The combination of chemical and mechanical stimulation seems possible, but does not show synergistic effects. In this study, a new model was successfully introduced in the field of PDLrelated regenerative research. Besides validating the 3D model to mimic an authentic PDL space, it also provided a useful and well-controlled approach to study cell response to mechanical loading and other stimuli.

show abstract

Regulation of Rat Bone Sialoprotein Gene Transcription by Enamel Matrix Derivative

Abstract: These studies have, therefore, identified EMD response elements in the rat BSP gene promoter that may mediates the effects of EMD on BSP gene transcription.

Cited by 45 publications

References 50 publications

Daily low-intensity pulsed ultrasound-mediated osteogenic differentiation in rat osteoblasts

Daily low-intensity pulsed ultrasound-mediated osteogenic differentiation in rat osteoblasts

Effects of a calcium phosphate cement on mineralized nodule formation compared with endodontic cements

A Three-Dimensional Cell Culture Model to Study the Mechano-Biological Behavior in Periodontal Ligament Regeneration

Contact Info

Product

Resources

About