Regulation of rat liver maturation in vitro by glucocorticoids.

Chou, Janice Y.; Wan, Y J; Sakiyama, T

doi:10.1128/mcb.8.1.203

Cited by 20 publications

(6 citation statements)

References 28 publications

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“…The increased concentration of circulating glucocorticoids in fetal serum just before birth may be a key regulatory signal for development of the liver. This view is supported by the recent demonstration that glucocorticoids can promote fetal liver maturation in vitro (8).…”

Section: Resultssupporting

confidence: 67%

Glucocorticoid and developmental regulation of amylase mRNAs in mouse liver cells.

et al. 1988

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We characterized alpha-amylase expression in the hepatoma cell line Hepa 1-6 and in normal mouse liver. Both Amy-1 and Amy-2 were expressed in Hepa 1-6 and were regulated by glucocorticoids. Transcription in the hepatoma cells was initiated at the same start sites as in mouse tissues. Glucocorticoid treatment increased the abundance of Amy-1 and Amy-2 transcripts by 10 to 20-fold. This increase was detected within 4 h and was maximal by 24 h. The pattern of amylase expression in this hepatoma cell line accurately reflects amylase expression in the liver in vivo. During liver development, we observed a large increase in the abundance of Amy-1 transcripts just before birth, at a time when circulating glucocorticoids are also elevated. Adult mouse liver expressed Amy-1 and Amy-2 at levels comparable to those of fully induced hepatoma cells. Liver is thus a likely source of both amylase isozymes in mouse serum. These studies demonstrate that Amy-2 expression is not limited to the pancreas but also occurs at a low level in liver cells.

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Section: Resultssupporting

confidence: 67%

Glucocorticoid and developmental regulation of amylase mRNAs in mouse liver cells.

et al. 1988

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“…The effects of glucocorticoids on the differentiation of bone-derived cells in vitro are not limited to cells of the osteoblast lineage Chou et al, 1988;Grigoriadis et al, 1988;Grigoriadis et al, 1989;Ringold et al, 1986;Violette et al, 19901. It is our experience that the conditions which favour osteogenic differentiation in cultures of rat marrow stromal cells (continuous culture in the presence of dexamethasone) also permit the differentiation of adipocytes. This conclusion is based upon the appearance of cells in the cultures having the morphological characteristics of adipocytes and the detection of the adipocyte specific aP-2 mRNA [Bernlohr et al, 1984;Hresko et al, 1988;unpublished observations].…”

Section: Isolation Of Osteoblast-like Cell Populations From Glass Framentioning

confidence: 98%

Formation of mineralized nodules by bone derived cells in vitro: A model of bone formation?

1993

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The identification of the factors which regulate the proliferation and differentiation of cells of the osteoblast lineage remains one of the major challenges in the field of bone cell biology. Although considerable progress has been made in the isolation and culture of cells of the osteoblast lineage from both animal and, more recently, human bone, uncertainties have persisted as to the extent to which these cell populations retain the ability to differentiate into functional osteoblasts in vitro. The formation in vitro of mineralized nodules that exhibit the morphological, ultrastructural and biochemical characteristics of embryonic/woven bone formed in vivo, represents the first evidence that the differentiation of functional osteoblasts can occur in cultures of isolated animal bone-derived cell populations. It is clear, however, that the culture conditions employed at present only permit a small number of cells to differentiate to the extent of being capable of organising their extracellular matrix into a structure that resembles that of bone. Moreover, it has generally been found that the reproducible mineralization of this extracellular matrix requires supplementation of the culture medium with mM concentrations of beta-GP, which raises doubts as to the physiological relevance of this process. The formation of nodules has also been observed in cultures of human bone-derived cells. As found in cultures of animal bone-derived cells, reproducible mineralization of these nodules will occur in the presence of beta-GP. We have shown, however, that in the presence of the long acting ascorbate analogue Asc-2-P, the formation and mineralization of nodules can occur in the absence of beta-GP. The nodules formed in human bone-derived cell cultures have yet to be characterized as rigorously as those formed in cultures of animal bone-derived cells and thus it remains to be shown that they resemble bone formed in vivo.

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“…On the other hand, the maturation of fetal hepatocytes can be monitored by the decreased expression of AFP and increased expression of albumin gene (Chou et al 1988). Both retinoic acid (RA) and glucocorticoids exert profound effects on liver specific gene expression and hepatocyte phenotype (Cook & Chiu 1986, Chou et al 1988, Wan et al 1994a). Previously, we have shown that all-írans-RA (i-RA), whose receptors belong to the steroid/thyroid hormone receptor family, enhanced the expression of AFP and albumin genes in McA-RH 8994 rat hepatoma cells.…”

Section: Introductionmentioning

confidence: 98%

9-cis-retinoic acid is more effective than all-trans-retinoic acid in upregulating expression of the α-fetoprotein gene

Wan

Pan

Wang

et al. 1995

Journal of Molecular Endocrinology

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In McA-RH 8994 rat hepatoma cells, all-trans-retinoic acid (t-RA) induces expression of the alpha-fetoprotein (AFP) and albumin genes and results in a phenotype similar to differentiated fetal hepatocytes. The present study elucidated the mechanism involved in AFP gene regulation mediated by retinoic acid. Northern blot analyses demonstrated that 9-cis-retinoic acid (c-RA), a ligand for retinoid x receptors (RXRs), also induced expression of the AFP gene in McA-RH 8994 cells. The induction was time- and dose-dependent. Northern blots and transfection assays using the 7.3 kb full-length regulatory region of the AFP gene demonstrated that c-RA was more effective than t-RA in regulating expression of the AFP gene. At 10(-7) M, c-RA increased AFP mRNA 5-fold and chloramphenicol acetyltransferase (CAT) activity 2.5-fold. In contrast, t-RA at a concentration of 10(-7) M exerted no significant effect; 10(-6) to 10(-5) M t-RA was needed to affect AFP gene expression. These data suggested that activation of RXRs is essential for the regulation of the AFP gene. Co-transfection experiments revealed that over-expression of RXR alpha in McA-RH 8994 cells further enhanced the CAT activity induced by c-RA. In addition, c-RA did not alter the half-life of AFP mRNA. Thus, RXR alpha may play a crucial role in transcriptional regulation of the AFP gene and in controlling hepatocyte phenotype.

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Regulation of rat liver maturation in vitro by glucocorticoids.

Cited by 20 publications

References 28 publications

Glucocorticoid and developmental regulation of amylase mRNAs in mouse liver cells.

Glucocorticoid and developmental regulation of amylase mRNAs in mouse liver cells.

Formation of mineralized nodules by bone derived cells in vitro: A model of bone formation?

9-cis-retinoic acid is more effective than all-trans-retinoic acid in upregulating expression of the α-fetoprotein gene

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