SummaryThis study aimed to examine the influence of exogenous application of the bioregulators indole acetic acid (IAA), indole butyric acid (IBA) and naphthalene acetic acid (NAA) on the vitamin and sugar content of tomatoes. Harvested fruits of the treated tomato genotypes, namely NHLy 11, NHLy 12, NHLy 13, NHLy 15 and NHLy 16, were analysed for these parameters. Spectrophotometric analysis results showed that IAA significantly increased (p0.05) the concentrations of -carotene, vitamin A and ascorbate in all the tested genotypes, with the highest values of 707.84 g·100 g -1 fwb, 118.21 g·100 g -1 RE and 20.36 g·100 g -1 fwb, respectively, in the NHLy16 genotype. The total sugar content was not significantly affected by the treatments. It is proposed that exogenous application of these bioregulators to plant foods such as tomatoes can improve their phytonutrient concentrations, promoting better public health.