Regulation of ROS–NF‐κB axis by tuna backbone derived peptide ameliorates inflammation in necrotizing enterocolitis

Zhang, Le; Fan, Jianfeng; He, Jingya; Chen, Wenjuan; Jin, Weilai; Zhu, Yuting; Sun, Hong; Li, Yawen; Shi, Yingzuo; Jing, Yulei; Wang, Xiaolei; Han, Song; Li, Zhengying

doi:10.1002/jcp.28133

Cited by 28 publications

(19 citation statements)

References 48 publications

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“…Cheng [22] showed that the small molecular peptides isolated and purified from the enzymatic hydrolysis of tuna boiled juice had great anti-inflammatory activity. Zhang [23] demonstrated that antioxidant peptides extracted from tuna bone protein enhanced the inflammatory response of necrotizing colitis caused by decreasing reactive oxygen species (ROS) and inhibiting the NF-κB pathway. Wang [24] illustrated that enzymatically hydrolyzed peptides from Skipjack (Katsuwonus pelamis) had a good regulating effect on inflammation and intestinal flora of UC model mice induced by DSS.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Tuna Bioactive Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice

et al. 2021

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Bioactive peptides isolated from marine organisms have shown to have potential anti-inflammatory effects. This study aimed to investigate the intestinal protection effect of low molecular peptides (Mw < 1 kDa) produced through enzymatic hydrolysis of tuna processing waste (tuna bioactive peptides (TBP)) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in BALB/c mice. Here, we randomly divided twenty-four male BALB/c mice into four groups: (i) normal (untreated), (ii) DSS-induced model colitis, (iii) low dose TBP+DSS-treated (200 mg/kg/d), and (iv) high dose TBP+DSS-treated groups (500 mg/kg/d). The results showed that TBP significantly reduced mice weight loss and improved morphological and pathological characteristics of colon tissues. In addition, it increased the activities of antioxidant enzymes (SOD and GSH-Px) and decreased inflammatory factors (LPS, IL-6, and TNF-α) expression. TBP increased the gene expression levels of some tight junction (TJ) proteins. Moreover, TBP increased the short-chain fatty acids (SCFAs) levels and the diversity and imbalance of intestinal flora. Therefore, TBP plays some protective roles in the intestinal tract by enhancing antioxidant and anti-inflammatory abilities of the body, improving the intestinal barrier and metabolic abnormalities, and adjusting intestinal flora imbalance.

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Section: Introductionmentioning

confidence: 99%

Protective Effect of Tuna Bioactive Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice

et al. 2021

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“…Silencing prolyl hydroxylase 2 (PHD2) could also alter the effect of bone marrow mesenchyml stem cells (BM-MSCs) via the NF-κB-dependent mechanism 48 . APTBP, an anti-oxidative peptide, could improve the NEC in ammation by inhibiting ROS-NF-κB axis 49 . In this study, we found that PDTC could ameliorate the in ammation, oxidative stress and intestinal permeability in NEC mice.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of SIRT1-mediated NF-κB Signal Pathway against Necrotizing Enterocolitis in Neonatal Mice

Zhang

Ren

Ding

et al. 2022

Preprint

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Objective To discover the mechanism of Sirtuin1 (SIRT1)-mediated NF-κB pathway in the protection of necrotizing enterocolitis (NEC) in neonatal mice. Methods Neonatal mice were treated with EX527 (an inhibitor of SIRT1) and/or pyrrolidine dithiocarbamate (PDTC, an inhibitor of NF-κB). The survival rate of mice was recorded. Hematoxylin & eosin (HE) staining was performed to observe the pathological changes of intestines. Furthermore, western blotting, Enzyme-linked immuno sorbent assay (ELISA), and real-time quantitative polymerase chain reaction (qRT-PCR) were conducted to measure the protein and gene expression, while corresponding kits to detect the levels of oxidative stress indicators. Results PDTC increased the survival rate of NEC mice. When compared with the NEC + EX527 + PDTC group, the histological score of NEC was higher in the NEC + EX527 group but was lower in the NEC + PDTC group. SIRT1 expression in the intestines of NEC mice was down-regulated, with an increase in p65 nuclear translocation. Also, MDA increased and GPx decreased in the intestines of NEC mice, with the up-regulations of IL-6, IL-1β and TNF-α, as well as the down-regulations of ZO-1, occludin and claudin-4 in the intestines. However, changes above could be improved by PDTC, which would be further reversed by EX527. Conclusion SIRT1 could mitigate inflammation and oxidative stress response and improve the intestinal permeability by mediating NF-κB pathway, playing an important role in the alleviation of NEC.

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“…Apoptosis of intestinal epithelial cells is linked to NEC progression [ 18 ]. Extrinsic lipopolysaccharide-induced reactive oxygen species accumulation results in NF- κ B activation and subsequent release of proinflammation cytokines, including IL-6 and IL-1 β , which are involved in NEC pathogenesis [ 19 , 20 ]. KEGG pathway analysis identified pathways that are mainly linked to NEC progression.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression Profiles and Functional Prediction of circRNAs in Necrotizing Enterocolitis

Pan

Chen

Yan

et al. 2021

BioMed Research International

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Circular RNAs (circRNAs), a novel type of noncoding RNAs, have been demonstrated to behave as microRNA (miRNA) sponges to exert their effects during pathological processes of diseases. However, the roles of circRNAs have not been explored in necrotizing enterocolitis (NEC). This study sought to identify differentially expressed circRNAs and predict their potential biological functions in NEC. circRNA expression profiles in terminal ileum from newborn rats with NEC and normal controls were explored using next-generation sequencing. In the NEC group, 53 circRNAs were significantly differentially expressed, including 9 upregulated and 44 downregulated. Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were conducted, and circRNA-miRNA interaction networks were generated to predict the potential roles of circRNAs in NEC progression. Further investigation revealed that most circRNAs include miRNA binding sites and that some are implicated in NEC development. In conclusion, this study’s findings demonstrate that differentially expressed circRNAs are involved in NEC development via their interactions with miRNAs, making them prospective targets for NEC diagnosis and treatment.

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Regulation of ROS–NF‐κB axis by tuna backbone derived peptide ameliorates inflammation in necrotizing enterocolitis

Cited by 28 publications

References 48 publications

Protective Effect of Tuna Bioactive Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice

Protective Effect of Tuna Bioactive Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice

Protective Effect of SIRT1-mediated NF-κB Signal Pathway against Necrotizing Enterocolitis in Neonatal Mice

Differential Expression Profiles and Functional Prediction of circRNAs in Necrotizing Enterocolitis

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