Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies

Bi, Jianjun; Hong, Jung-Pyo; Zhou, Chunqin; Gao, Peng; Han, Fujun; Li, Gang; Zhang, Shiwei

doi:10.3233/thc-thc228034

Cited by 6 publications

(3 citation statements)

References 40 publications

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“…Further, we demonstrated that C2C12 on fungal carriers expressed key markers of myogenesis in response to media-induced differentiation. MYOD1 , MYOG , and MYH are myogenic regulatory factors that regulate the development of functional embryonic skeletal fiber muscle differentiation 40 , 41 . MYH1 and MYH2 are genes that encode MyHC-2X and MyHC-2A, respectively.…”

Section: Discussionmentioning

confidence: 99%

Edible mycelium as proliferation and differentiation support for anchorage-dependent animal cells in cultivated meat production

Ogawa,

Kermani,

Huynh

et al. 2024

npj Sci Food

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Cultivated meat production requires bioprocess optimization to achieve cell densities that are multiple orders of magnitude higher compared to conventional cell culture techniques. These processes must maximize resource efficiency and cost-effectiveness by attaining high cell growth productivity per unit of medium. Microcarriers, or carriers, are compatible with large-scale bioreactor use, and offer a large surface-area-to-volume ratio for the adhesion and proliferation of anchorage-dependent animal cells. An ongoing challenge persists in the efficient retrieval of cells from the carriers, with conflicting reports on the effectiveness of trypsinization and the need for additional optimization measures such as carrier sieving. To surmount this issue, edible carriers have been proposed, offering the advantage of integration into the final food product while providing opportunities for texture, flavor, and nutritional incorporation. Recently, a proof of concept (POC) utilizing inactivated mycelium biomass derived from edible filamentous fungus demonstrated its potential as a support structure for myoblasts. However, this POC relied on a model mammalian cell line combination with a single mycelium species, limiting realistic applicability to cultivated meat production. This study aims to advance the POC. We found that the species of fungi composing the carriers impacts C2C12 myoblast cell attachment—with carriers derived from Aspergillus oryzae promoting the best proliferation. C2C12 myoblasts effectively differentiated on mycelium carriers when induced in myogenic differentiation media. Mycelium carriers also supported proliferation and differentiation of bovine satellite cells. These findings demonstrate the potential of edible mycelium carrier technology to be readily adapted in product development within the cultivated meat industry.

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Section: Discussionmentioning

confidence: 99%

Edible mycelium as proliferation and differentiation support for anchorage-dependent animal cells in cultivated meat production

Ogawa,

Kermani,

Huynh

et al. 2024

npj Sci Food

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“…Therefore, the C2C12 cell line provides an invaluable tool to study the molecular biology and mechanisms of myogenic regulation [43,44], muscle differentiation [45], regeneration, and muscle atrophy [41].…”

Section: C2c12 Cell Linementioning

confidence: 99%

In Vitro Models for Cancer-Associated Cachexia: The Complex Modelling of a Multiorgan Syndrome

Meireles,

Medeiros,

Cerqueira

2024

Applied Sciences

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Cancer-associated cachexia is a multifactorial syndrome characterised by systemic inflammation and hypermetabolism that affects different tissues and organs. Is characterised by progressive and irreversible weight loss, mainly due to skeletal muscle wasting and often accompanied by loss of fat mass. Due to its complexity, and lack of effective treatment, this syndrome is a sign of poor prognosis in cancer patients. Cellular models constitute a valuable and powerful tool offering insights into the molecular pathways and cellular responses associated with cancer cachexia. Currently, there are robust and widely used cell lines used to establish models to study the pathophysiology of muscle wasting and adipose tissue loss. Various methods can be used to induce the cachectic phenotype in the cells, utilising genetic engineering or different inducing agents such as hormones, inflammatory factors and chemotherapeutic drugs. The available experimental data on their metabolic properties and transcriptional and proteomic profiles allows the selection of the most suitable research model to replicate the relevant aspects of cachexia. In this review, we make an overview of the in vitro models used to study biological aspects of cancer-associated cachexia and analyse their strengths and limitations in replicating the complex physiological environment and pathological processes of the syndrome. Herein, we also briefly approach the difficulty of modelling the contribution of different organs and crosstalk between different tissues.

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“…PEMF has been shown to increase the myotube diameter and fusion index, activating TRPC1-mediated calcium entry and mitochondrial respiration downstream of calcineurin-dependent transcriptional regulation and P300/CBP-associated factor (PCAF)-epigenetic signaling, which was previously found to be involved in myogenesis [19,20]. Even less is known about the ability of PEMF to accelerate human SkMC regeneration after injury or the chemical mechanism behind this effect [20][21][22]. Here, we studied the potentiality of PEMF to stimulate the early regeneration of human SkMC.…”

Section: Introductionmentioning

confidence: 99%

Pulsed Electromagnetic Fields Induce Skeletal Muscle Cell Repair by Sustaining the Expression of Proteins Involved in the Response to Cellular Damage and Oxidative Stress

Maiullari,

Cicirelli,

Picerno

et al. 2023

IJMS

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Pulsed electromagnetic fields (PEMF) are employed as a non-invasive medicinal therapy, especially in the orthopedic field to stimulate bone regeneration. However, the effect of PEMF on skeletal muscle cells (SkMC) has been understudied. Here, we studied the potentiality of 1.5 mT PEMF to stimulate early regeneration of human SkMC. We showed that human SkMC stimulated with 1.5 mT PEMF for four hours repeated for two days can stimulate cell proliferation without inducing cell apoptosis or significant impairment of the metabolic activity. Interestingly, when we simulated physical damage of the muscle tissue by a scratch, we found that the same PEMF treatment can speed up the regenerative process, inducing a more complete cell migration to close the scratch and wound healing. Moreover, we investigated the molecular pattern induced by PEMF among 26 stress-related cell proteins. We found that the expression of 10 proteins increased after two consecutive days of PEMF stimulation for 4 h, and most of them were involved in response processes to oxidative stress. Among these proteins, we found that heat shock protein 70 (HSP70), which can promote muscle recovery, inhibits apoptosis and decreases inflammation in skeletal muscle, together with thioredoxin, paraoxonase, and superoxide dismutase (SOD2), which can also promote skeletal muscle regeneration following injury. Altogether, these data support the possibility of using PEMF to increase SkMC regeneration and, for the first time, suggest a possible molecular mechanism, which consists of sustaining the expression of antioxidant enzymes to control the important inflammatory and oxidative process occurring following muscle damage.

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Regulation of skeletal myogenesis in C2C12 cells through modulation of Pax7, MyoD, and myogenin via different low-frequency electromagnetic field energies

Cited by 6 publications

References 40 publications

Edible mycelium as proliferation and differentiation support for anchorage-dependent animal cells in cultivated meat production

Edible mycelium as proliferation and differentiation support for anchorage-dependent animal cells in cultivated meat production

In Vitro Models for Cancer-Associated Cachexia: The Complex Modelling of a Multiorgan Syndrome

Pulsed Electromagnetic Fields Induce Skeletal Muscle Cell Repair by Sustaining the Expression of Proteins Involved in the Response to Cellular Damage and Oxidative Stress

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