Regulation of stem cell factor expression in inflammation and asthma

Silva, Carla A. Da; Frossard, Nelly

doi:10.1590/s0074-02762005000900025

Cited by 16 publications

(9 citation statements)

References 67 publications

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“…6,10 -14,47,52 Moreover recent evidence suggests that SCF may be important also in human asthma and is highly expressed after lung transplantation. [52][53][54] However the precise mechanism of how these factors could recruit and activate fibroblasts in the remodeling airway in vivo remains poorly understood. Recent reports on the plasticity of adult bone marrow stem cells and the presence of circulating fibroblast-like cells known as fibrocytes, have engendered considerable interest on the potential extrapulmonary origin of fibroblasts and myofibroblasts in the remodeling airway.…”

Section: Discussionmentioning

confidence: 99%

Role of Stem Cell Factor and Bone Marrow-Derived Fibroblasts in Airway Remodeling

Dolgachev

Ullenbruch

Lukacs

et al. 2009

The American Journal of Pathology

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Recent evidence suggests that bone marrow-derived fibroblasts are involved in airway remodeling in asthma, but the role and mechanism of recruitment of these fibroblasts remains unclear. Stem cell factor (SCF), a key factor in the propagation of hematopoietic stem cells, is important in the process of airway remodeling as well. To test the hypothesis that SCF is involved in the recruitment and differentiation of bone marrow-derived progenitor cells, GFP-bone marrow chimeric mice were created. These mice were then sensitized and chronically challenged with cockroach antigen to induce chronic airway disease. Fluorescence microscopy revealed an influx of significant numbers of GFP-expressing fibroblasts in the airways of these mice, which was confirmed by flow cytometric analysis of cells co-expressing both GFP and collagen I. These cells preferentially expressed c-kit, interleukin-31 receptor, and telomerase reverse transcriptase when compared with control lung-derived fibroblasts. Interestingly, SCF stimulated interleukin-31 receptor expression in bone marrow cells, whereas interleukin-31 strongly induced telomerase reverse transcriptase expression in fibroblasts. Treatment with neutralizing antibodies to SCF significantly reduced airway remodeling and suppressed the recruitment of these bone marrow-derived cells to the lung. Thus SCF in conjunction with interleukin-31 may play a significant role in airway remodeling by promoting the recruitment of bone marrow-derived fibroblast precursors into the lung with the capacity to promote lung myofibroblast differentiation. (Am J

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Section: Discussionmentioning

confidence: 99%

Role of Stem Cell Factor and Bone Marrow-Derived Fibroblasts in Airway Remodeling

Dolgachev

Ullenbruch

Lukacs

et al. 2009

The American Journal of Pathology

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“…That is, increased SCF expression in the bronchi of a patient with asthma would be associated with increased number and activation of mast cells [25]. …”

Section: Discussionmentioning

confidence: 99%

Association of stem cell factor gene expression with severity and atopic state in patients with bronchial asthma

et al. 2017

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Background Bronchial asthma is a chronic inflammatory and remodeling disorder of the airways, in which many cells, cellular elements, and cytokines play important roles. Stem cell factor (SCF) may contribute to the inflammatory changes occurring in asthma. We aimed to show the expression of SCF gene in patients with asthma as a means of diagnosis and its association with severity and atopic state in these patients.MethodsThis study was carried out on 80 subjects, 50 asthmatic patients and 30 age and gender matched healthy control persons. They were subjected to full history taking, general and local chest examination, spirometric measurements (pre and post broncodilators) using a spirometer, serum IgE, and real time PCR for assessment of SCF mRNA expression.ResultsThis study showed significant difference between the studied groups regarding pulmonary function tests (P < 0.001). Asthmatic patients had significant higher SCF expression compared to control (P < 0.001), also atopic patients vs non atopic (P = 0.03) and severe asthmatic patients vs mild ones (P < 0.001). SCF expression at cut off point (0.528) is sufficient to discriminate asthmatic patients from control while at cut off point (1.84) for discrimination of atopic patients from non-atopic patients and at cut off point (1.395) for discrimination of severe asthmatic patients from mild ones. A significant negative correlation between SCF expression and inhaled steroid while significant positive correlation with serum IgE was found.ConclusionMeasuring SCF mRNA expression can be used as an efficient marker for evaluation of atopy and detection of severity of bronchial asthma.

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“…Another major molecule showing significant elevation was pro-inflammatory cytokineTNF-α; this plays a major role in stimulating neutrophil degranulation, respiratory burst, and release of platelet activating factor (PAF) (Ogura, Furukawa, Tada, Ikeda, & Yamashita, 2008). SCF plays a major role in mast cell degranulation (Lukacs et al, 1996) and the differentiation of CD34 + cells into mast cells in the upper respiratory tract (Da Silva & Frossard, 2005). TNF-α was also significantly elevated in the chinchilla middle ear after pneumococcus inoculation but at a later time point (72 hrs) following infection (Sato et al, 1999).…”

Section: Nthi Infection In Preinflamed Middle Ear Causes Changes Inmentioning

confidence: 99%

“…Chemokines like SCF and TPO were elevated in NTHi+ve middle-ear fluids; these not only induce inflammation but also regulate stem cell function. SCF plays a major role in mast cell degranulation (Lukacs et al, 1996) and the differentiation of CD34 + cells into mast cells in the upper respiratory tract (Da Silva & Frossard, 2005). Similarly, TPO plays a role in haematopoietic stem cell differentiation (Lupia, Goffi, Bosco, & Montrucchio, 2012a).…”

Section: Nthi Infection In Preinflamed Middle Ear Causes Changes Inmentioning

confidence: 99%

Cellular content plays a crucial role in Non‐typeableHaemophilus influenzaeinfection of preinflamedJunbomouse middle ear

Vikhe

Purnell

Brown

et al. 2018

Cellular Microbiology

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Non‐typeable Haemophilus influenzae (NTHi) is a major pathogen causing acute otitis media (AOM). The relationship between the cellular content of the middle ear fluid (MEF) during AOM and infection of NTHi is poorly understood. Using the Junbo mouse, a characterised NTHi infection model, we analysed the cellular content of MEF and correlated the data with NTHi titres. The MEF of the Junbo mouse was heterogeneous between ears and was graded from 1 to 5; 1 being highly serous/clear and 5 being heavily viscous/opaque. At seven‐day post‐intranasal inoculation, NTHi was not found in grade‐1 or 2 fluids, and the proportion of MEF that supported NTHi increased with the grade. Analyses by flow cytometry indicated that the cellular content was highest in grade‐4 and 5 fluids, with a greater proportion of necrotic cells and a low‐live cell count. NTHi infection of the middle ear increased the cell count and led to infiltration of immune cells and changes in the cytokine and chemokine levels. Following NTHi inoculation, high‐grade infected MEFs had greater neutrophil infiltration whereas monocyte infiltration was significantly higher in serous noninfected low‐grade fluids. These data underline a role for immune cells, specifically monocytes and neutrophils, and cell necrosis in NTHi infection of the Junbo mouse middle ear.

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Regulation of stem cell factor expression in inflammation and asthma

Abstract: Stem cell factor (SCF) is

Cited by 16 publications

References 67 publications

Role of Stem Cell Factor and Bone Marrow-Derived Fibroblasts in Airway Remodeling

Role of Stem Cell Factor and Bone Marrow-Derived Fibroblasts in Airway Remodeling

Association of stem cell factor gene expression with severity and atopic state in patients with bronchial asthma

Cellular content plays a crucial role in Non‐typeableHaemophilus influenzaeinfection of preinflamedJunbomouse middle ear

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