Regulation of Sugar Uptake, Glycolysis, and the Pentose Phosphate Pathway in Corynebacterium glutamicum

Teramoto, Haruhiko; Inui, Masayuki

doi:10.1007/978-3-642-29857-8_9

Cited by 3 publications

(4 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A reduction of glucose uptake in presence of acetate was also seen for a valine overproducing strain . It has been further shown that SugR act as transcriptional repressor of l ‐lactate dehydrogenase (Ldh) . However, in case of elevated levels of SugR, they would probably not negatively affect the expression of ldh .…”

Section: Resultsmentioning

confidence: 97%

“…Transport proteins of the carbohydrate specific uptake systems for glucose (PtsG and PtsI) are slightly reduced. However, disturbances caused by diauxic growth are uncertain, as the presence of glucose does not reduce the uptake of other carbohydrates . Amino acids and other organic compounds could also affect substrate uptake.…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, the glyceraldehyde‐3‐phosphate dehydrogenase (GapA) level was elevated under oscillatory cultivation cultivations. It was identified as being essential for growth on glycolytic carbon sources .…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Performance loss of Corynebacterium glutamicum cultivations under scale‐down conditions using complex media

Lemoine

Limberg

Kästner

et al. 2016

Engineering in Life Sciences

View full text Add to dashboard Cite

Performance loss of Corynebacterium glutamicum cultivations under scale-down conditions using complex mediaSubstrate and oxygen gradients appear in industrial-scale fed-batch processes due to limitations in the achievable power input and concomitantly increased mixing times. In order to mimic these gradients at lab scale, scale-down reactors are applied. Previous studies in such reactor systems suggest that Corynebacterium glutamicum is robust against oscillatory oxygen and substrate availability in relation to growth and side product accumulation. Usually, defined mineral salt media are applied contrary to the industrial case, in which complex media containing different carbon sources are used. Therefore, this study investigated the cultivation performance using complex medium based on sucrose, molasses, and corn steep liquor in a threecompartment scale-down reactor. The reactor consisted of a stirred tank and two plug flow reactor modules. This approach was applied based on assumptions of gradient distributions in bottom-fed bioreactors. A drastic reduction of growth and volumetric product yield of a cadaverine producing strain was observed while several short chain fatty acids accumulated, among them l-lactate and acetate. Growth was depleted after several hours of cultivation, while the substrate uptake rate was reduced by 20%. Hence, the main carbon source sucrose accumulated after 10 h of fed-batch cultivation. Despite growth cessation, neither reduction of cell vitality nor increased cell lysis were observed. IntroductionAlthough progress has been made in the field of parallel automated bioprocess development, which includes several on-, at-, and offline analyses and model-based strategies to identify the best strain and cultivation conditions, scale-up of a bioprocess

show abstract

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Performance loss of Corynebacterium glutamicum cultivations under scale‐down conditions using complex media

Lemoine

Limberg

Kästner

et al. 2016

Engineering in Life Sciences

View full text Add to dashboard Cite

show abstract

“…Recent advances in metabolic engineering of C. glutamicum open up new possibilities for efficient utilization of substrates containing mixtures of D-glucose, D-xylose, and L-arabinose (9)(10)(11)(12). Understanding of the unique transcriptional regulatory system of sugar metabolism genes in C. glutamicum (13,14) highlights a potential avenue toward optimization of utilization of sugar mixtures derived from a variety of lignocellulosic feedstocks.…”

mentioning

confidence: 99%

AraR, an l -Arabinose-Responsive Transcriptional Regulator in Corynebacterium glutamicum ATCC 31831, Exerts Different Degrees of Repression Depending on the Location of Its Binding Sites within the Three Target Promoter Regions

2015

Self Cite

View full text Add to dashboard Cite

In Corynebacterium glutamicum ATCC 31831, a LacI-type transcriptional regulator AraR, represses the expression of L-arabinose catabolism (araBDA), uptake (araE), and the regulator (araR) genes clustered on the chromosome. AraR binds to three sites: one (BS B ) between the divergent operons (araBDA and galM-araR) and two (BS E1 and BS E2 ) upstream of araE. L-Arabinose acts as an inducer of the AraR-mediated regulation. Here, we examined the roles of these AraR-binding sites in the expression of the AraR regulon. BS B mutation resulted in derepression of both araBDA and galM-araR operons. The effects of BS E1 and/or BS E2 mutation on araE expression revealed that the two sites independently function as the cis elements, but BS E1 plays the primary role. However, AraR was shown to bind to these sites with almost the same affinity in vitro. Taken together, the expression of araBDA and araE is strongly repressed by binding of AraR to a single site immediately downstream of the respective transcriptional start sites, whereas the binding site overlapping the ؊10 or ؊35 region of the galM-araR and araE promoters is less effective in repression. Furthermore, downregulation of araBDA and araE dependent on L-arabinose catabolism observed in the BS B mutant and the AraR-independent araR promoter identified within galM-araR add complexity to regulation of the AraR regulon derepressed by L-arabinose. IMPORTANCECorynebacterium glutamicum has a long history as an industrial workhorse for large-scale production of amino acids. An important aspect of industrial microorganisms is the utilization of the broad range of sugars for cell growth and production process. Most C. glutamicum strains are unable to use a pentose sugar L-arabinose as a carbon source. However, genes for L-arabinose utilization and its regulation have been recently identified in C. glutamicum ATCC 31831. This study elucidates the roles of the multiple binding sites of the transcriptional repressor AraR in the derepression by L-arabinose and thereby highlights the complex regulatory feedback loops in combination with L-arabinose catabolism-dependent repression of the AraR regulon in an AraR-independent manner. L ignocellulosic biomass from agricultural and agro-industrial residues represents one of the important renewable resources expected to be used for the industrial production of biofuels and biochemicals (1, 2). However, significant amounts of pentose sugars derived from its hemicellulose component are one major technical hurdle in the use for economically feasible bioprocesses. These sugars, such as D-xylose and L-arabinose, are not efficiently utilized by conventional and industrial microorganisms, and improvement of the pentose sugar metabolic pathways by genetic engineering is often limited by preferential utilization of a most abundant and favorable hexose sugar, D-glucose, in lignocellulosic biomass (3).Corynebacterium glutamicum is a Gram-positive actinobacterium with a high GϩC content in its genomic DNA. It has a long history as an industri...

show abstract

Corynebacterium glutamicum for Sustainable Bioproduction: From Metabolic Physiology to Systems Metabolic Engineering

Becker

Gießelmann

Hoffmann

et al. 2016

Synthetic Biology – Metabolic Engineering

View full text Add to dashboard Cite

Since its discovery 60 years ago, Corynebacterium glutamicum has evolved into a workhorse for industrial biotechnology. Traditionally well known for its remarkable capacity to produce amino acids, this Gram-positive soil bacterium, has become a flexible, efficient production platform for various bulk and fine chemicals, materials, and biofuels. The central turnstile of all these achievements is our excellent understanding of its metabolism and physiology. This knowledge base, together with innovative systems metabolic engineering concepts, which integrate systems and synthetic biology into strain engineering, has upgraded C. glutamicum into one of the most successful industrial microorganisms in the world.

show abstract

Regulation of Sugar Uptake, Glycolysis, and the Pentose Phosphate Pathway in Corynebacterium glutamicum

Cited by 3 publications

References 52 publications

Performance loss of Corynebacterium glutamicum cultivations under scale‐down conditions using complex media

Performance loss of Corynebacterium glutamicum cultivations under scale‐down conditions using complex media

AraR, an l -Arabinose-Responsive Transcriptional Regulator in Corynebacterium glutamicum ATCC 31831, Exerts Different Degrees of Repression Depending on the Location of Its Binding Sites within the Three Target Promoter Regions

Corynebacterium glutamicum for Sustainable Bioproduction: From Metabolic Physiology to Systems Metabolic Engineering

Contact Info

Product

Resources

About