Regulation of the Actin Cytoskeleton by an Interaction of IQGAP Related Protein GAPA with Filamin and Cortexillin I

Mondal, Subhanjan; Burgute, Bhagyashri D.; Rieger, D.; Müller, Rolf; Rivero, Francisco; Faix, Jan; Schleicher, Michael; Noegel, Angelika A.

doi:10.1371/journal.pone.0015440

Cited by 26 publications

(38 citation statements)

References 58 publications

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“…This stress-dependent myosin II accumulation results from cooperative interactions between the actin-bound myosin heads and the actin-bundling protein cortexillin I [4]. Scaffolding proteins IQGAP1 and IQGAP2 bind to cortexillin I [12–14] and regulate myosin II accumulation [6]. IQGAP1 inhibits myosin II recruitment, while IQGAP2 relieves this inhibition.…”

Section: Resultsmentioning

confidence: 99%

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Mechanical Stress and Network Structure Drive Protein Dynamics during Cytokinesis

Srivastava

Robinson

2015

Current Biology

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Summary Cell shape changes associated with processes like cytokinesis and motility proceed on several second time-scales, but are derived from molecular events, including protein-protein interactions, filament assembly, and force generation by molecular motors, all of which occur much faster [1–4]. Therefore, defining the dynamics of such molecular machinery is critical for understanding cell shape regulation. In addition to signaling pathways, mechanical stresses also direct cytoskeletal protein accumulation [5–7]. A myosin II-based mechanosensory system controls cellular contractility and shape during cytokinesis and under applied stress [6, 8]. In Dictyostelium, this system tunes myosin II accumulation by feedback through the actin network, particularly through the crosslinker cortexillin I. Cortexillin-binding IQGAPs are major regulators of this system. Here, we defined the short time-scale dynamics of key cytoskeletal proteins during cytokinesis and under mechanical stress using fluorescence recovery after photobleaching and fluorescence correlation spectroscopy, to examine the dynamic interplay between these proteins. Equatorially enriched proteins including cortexillin I, IQGAP2, and myosin II recovered much more slowly than actin and polar crosslinkers. The mobility of equatorial proteins was greatly reduced at the furrow compared to the interphase cortex, suggesting their stabilization during cytokinesis. This mobility shift did not arise from a single biochemical event, but rather from a global inhibition of protein dynamics by mechanical stress-associated changes in the cytoskeletal structure. Mechanical tuning of contractile protein dynamics provides robustness to the cytoskeletal framework responsible for regulating cell shape and contributes to cytokinesis fidelity.

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Section: Resultsmentioning

confidence: 99%

“…Because IQGAP1 and IQGAP2 interact with distinct domains of cortexillin I [12–14], we also studied cortexillin I dynamics in the iqg1/2 double mutant. Here, cortexillin I still showed faster recovery at the furrow as compared to WT, similar to iqg2 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Mechanical Stress and Network Structure Drive Protein Dynamics during Cytokinesis

Srivastava

Robinson

2015

Current Biology

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“…It promotes actin ring formation by binding to actin filaments via its CHD (Calponin Homology Domain) [24, 25, 27, 76, 112], and it is required for the localization or maintenance of Myo1 at the division site during cytokinesis [25]. Both roles appear conserved in fission yeast [86, 87, 89, 113] and Dictyostelium [88, 109, 114]. …”

Section: Actomyosin Ring Assemblymentioning

confidence: 99%

Mechanics and regulation of cytokinesis in budding yeast

Bhavsar-Jog

2017

Seminars in Cell & Developmental Biology

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Cytokinesis is essential for the survival of all organisms. It requires concerted functions of cell signaling, force production, exocytosis, and extracellular matrix remodeling. Due to the conservation in core components and mechanisms between fungal and animal cells, the budding yeast Saccharomyces cerevisiae has served as an attractive model for studying this fundamental process. In this review, we discuss the mechanics and regulation of distinct events of cytokinesis in budding yeast, including the assembly, constriction, and disassembly of the actomyosin ring, septum formation, abscission, and their spatiotemporal coordination. We also highlight the key concepts and questions that are common to animal and fungal cytokinesis.

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“…Several proteins have been identified as cortexillin binding partners. Cortexillin I interacts with RacI, IQGAPs, and GAPA forming a complex, which plays a key role in cytokinesis and cell migration Mondal et al, 2010). Furthermore, an interaction with Rap-GAP1 has been demonstrated.…”

Section: Discussionmentioning

confidence: 99%

“…The last positively charged nonapeptide motif has a phosphatidylinositol (4,5)bisphosphate (PIP2)-binding site . Cortexillin I forms a complex with RacI and the IQGAPs DGAP1 and GAPA (Faix et al, 2001;Mondal et al, 2010). The IQGAP/cortexillin complexes are involved in regulating cortical mechanics.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Localization of the Actin-Bundling Protein Cortexillin I during Cell Migration

Cha

Jeon

2011

Molecules and Cells

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Cortexillins are actin-bundling proteins that play a critical role in regulating cell morphology and actin cytoskeleton reorganization in Dictyostelium. Here, we investigated dynamic subcellular localization of cortexillin I in chemotaxing Dictyostelium cells. Most of the cortexillin I was enriched on the lateral sides of moving cells. Upon chemoattractant stimulation, cortexillin I was rapidly released from the cortex followed by a transient translocation to the cell cortex with a peak at ~5 s and a subsequent decrease to basal levels, indicating that localization of cor-texillin I at the cortex in chemotaxing cells is controlled by two more signaling components, one for the initial delocalization from the cortex and another for the translocation to the cortex ~5 s after chemoattractant stimulation. Loss of cortexillins leads to reduced cell polarity and an in-creased number of lateral pseudopodia during chemotaxis, suggesting that cortexillins play an inhibitory role in producing pseudopodia along the lateral sides of the cell. Cells lacking cortexillins displayed extended chemoattrac-tantmediated Arp2/3 complex translocation kinetics to the cortex. Our present study provides a new insight into the function of cortexillins during reorganization of the actin cytoskeleton and cell migration.

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Regulation of the Actin Cytoskeleton by an Interaction of IQGAP Related Protein GAPA with Filamin and Cortexillin I

Cited by 26 publications

References 58 publications

Mechanical Stress and Network Structure Drive Protein Dynamics during Cytokinesis

Mechanical Stress and Network Structure Drive Protein Dynamics during Cytokinesis

Mechanics and regulation of cytokinesis in budding yeast

Dynamic Localization of the Actin-Bundling Protein Cortexillin I during Cell Migration

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