Regulation of the antioxidant system in cells of the fission yeast Schizosaccharomyces pombe after combined treatment with patulin and citrinin

Papp, Gábor; Máté, Gábor; Mike, Nóra; Gazdag, Zoltán; Pesti, Miklós

doi:10.1016/j.toxicon.2015.12.021

Cited by 6 publications

(2 citation statements)

References 60 publications

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“…Detection of intracellular NO and ROS. Intracellular NO and ROS were detected as described previously (40), with some modifications. Briefly, fission yeast cells were grown in EMM until the OD 660 reached 0.3 and were then washed three times with 1ϫ phosphate-buffered saline (PBS).…”

Section: Methodsmentioning

confidence: 99%

Dysfunction of Prohibitin 2 Results in Reduced Susceptibility to Multiple Antifungal Drugs via Activation of the Oxidative Stress-Responsive Transcription Factor Pap1 in Fission Yeast

Liu

Yao

Jiang

et al. 2018

Antimicrob Agents Chemother

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The fight against resistance to antifungal drugs requires a better understanding of the underlying cellular mechanisms. In order to gain insight into the mechanisms leading to antifungal drug resistance, we performed a genetic screen on a model organism, , to identify genes whose overexpression caused resistance to antifungal drugs, including clotrimazole and terbinafine. We identified the gene, encoding a highly conserved mitochondrial protein, prohibitin (Phb2), as a novel determinant of reduced susceptibility to multiple antifungal drugs. Unexpectedly, deletion of the gene also exhibited antifungal drug resistance. Overexpression of the gene failed to cause drug resistance when the gene, encoding an oxidative stress-responsive transcription factor, was deleted. Furthermore, mRNA expression was significantly increased when the gene was overexpressed or deleted. Importantly, either overexpression or deletion of the gene stimulated the synthesis of NO and reactive oxygen species (ROS), as measured by the cell-permeant fluorescent NO probe DAF-FM DA (4-amino-5-methylamino-2',7'-difluorofluorescein diacetate) and the ROS probe DCFH-DA (2',7'-dichlorodihydrofluorescein diacetate), respectively. Taken together, these results suggest that Phb2 dysfunction results in reduced susceptibility to multiple antifungal drugs by increasing NO and ROS synthesis due to dysfunctional mitochondria, thereby activating the transcription factor Pap1 in fission yeast.

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Section: Methodsmentioning

confidence: 99%

Dysfunction of Prohibitin 2 Results in Reduced Susceptibility to Multiple Antifungal Drugs via Activation of the Oxidative Stress-Responsive Transcription Factor Pap1 in Fission Yeast

Liu

Yao

Jiang

et al. 2018

Antimicrob Agents Chemother

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“…Patulin can cause an unbalanced redox state in cells through the accumulation of reactive oxygen species (ROS), which plays a crucial role in the adaptation processes ( Papp et al, 2012 , 2016 ). Research demonstrated that treatment with 500 μM PAT significantly increased the specific activities of Cu/Zn superoxide dismutase, catalase, and glutathione S -transferase in order to protect the cells against the ROS-induced unbalanced redox state ( Papp et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

The Response of Rhodotorula mucilaginosa to Patulin Based on Lysine Crotonylation

Yang

Apaliya

et al. 2018

Front. Microbiol.

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Patulin (PAT) is a mycotoxin produced by some Penicillium, Aspergillus, and Byssochlamys species. Rhodotorula mucilaginosa is able to degrade PAT in vivo as well as in vitro, up till date, the process and molecular mechanism(s) involved patulin degradation still remains unknown. Protein lysine crotonylation (Kcr) plays an important role in regulating chromatin dynamics, gene expression, and metabolic pathways in mammals and eukaryotes. Investigation of the Kcr changes accompanying degradation of patulin in R. mucilaginosa were observed to investigate the mechanisms of patulin inhibition. Tandem mass tag (TMT) labeling and Kcro affinity enrichment, followed by high-resolution LC-MS/MS analysis, were used to perform quantitative lysine crotonylome analysis on R. mucilaginosa. Consequently, 1691 lysine crotonylation sites in 629 protein groups were identified, among which we quantified 1457 sites in 562 proteins. Among the quantified proteins, 79 and 46 crotonylated proteins were up-regulated and down-regulated, respectively. The differentially up expressed modified proteins were mainly involved in tricarboxylic acid cycle and gluconeogenic pathway. The differentially down expressed Kcr proteins were mainly classified to ribosome and carbohydrate transport and metabolism. Bioinformatic analyses were performed to annotate the quantifiable lysine crotonylated targets. Moreover, interaction networks and high confidence domain architectures of crotonylated proteins were investigated with the aid of bioinformatic tools, and these results showed that there was an increase in the number of yeasts with crotonylated proteins. The results also provided information on the various roles of crotonylation, which are involved in PAT degradation.

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The effect of Rhodotorula mucilaginosa on degradation of citrinin production by Penicillium digitatum and its toxin in vitro

et al. 2019

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Regulation of the antioxidant system in cells of the fission yeast Schizosaccharomyces pombe after combined treatment with patulin and citrinin

Cited by 6 publications

References 60 publications

Dysfunction of Prohibitin 2 Results in Reduced Susceptibility to Multiple Antifungal Drugs via Activation of the Oxidative Stress-Responsive Transcription Factor Pap1 in Fission Yeast

Dysfunction of Prohibitin 2 Results in Reduced Susceptibility to Multiple Antifungal Drugs via Activation of the Oxidative Stress-Responsive Transcription Factor Pap1 in Fission Yeast

The Response of Rhodotorula mucilaginosa to Patulin Based on Lysine Crotonylation

The effect of Rhodotorula mucilaginosa on degradation of citrinin production by Penicillium digitatum and its toxin in vitro

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