Regulation of the black bullhead hepatic β-adrenoceptors

Dugan, Stephen G.; Chen, Xi; Nickerson, James G.; Montpetit, Colin J.; Moon, Thomas W.

doi:10.1016/j.cbpb.2007.09.016

Cited by 16 publications

(8 citation statements)

References 32 publications

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“…β-ARs in fish have previously been reported to be encoded by adrb2 (Nickerson et al, 2001), adrb3a and adrb3b (Nickerson et al, 2003) in Oncorhynchus mykiss (rainbow trout), and adrb1, adrb2 and adrb3 in Ameiurus melas (black bullhead) (Dugan et al, 2008). The amino acid sequences of rainbow trout and black bullhead adrb2 are more similar to zebrafish adrb2b than adrb2a (Supplementary Table 3), suggesting that rainbow trout and black bullhead adrb2 may be orthologs of zebrafish adrb2b.…”

Section: Cdna Cloning Of Zebrafish β-Arsmentioning

confidence: 89%

“…Another possibility is that knock-down of adrb2a may reduce metabolism in yolk. It has been shown that adrb2 expressed in rainbow trout hepatocytes regulates glucose and FFA metabolism (Van Heeswijk et al, 2006;Dugan et al, 2008). Knock-down of adrb2a may reduce glucose and FFA metabolism in both liver and yolk.…”

Section: Comparison Of Zebrafish β-Ars With Those Of Other Speciesmentioning

confidence: 98%

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Zebrafish β-adrenergic receptor mRNA expression and control of pigmentation

Wang

Nishimura

Shimada

et al. 2009

Gene

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Section: Cdna Cloning Of Zebrafish β-Arsmentioning

confidence: 89%

Section: Comparison Of Zebrafish β-Ars With Those Of Other Speciesmentioning

confidence: 98%

Zebrafish β-adrenergic receptor mRNA expression and control of pigmentation

Wang

Nishimura

Shimada

et al. 2009

Gene

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“…Similarly, Fraysse et al () observed that exposure to propranolol decreased the heart rate of zebrafish embryos. Competitive binding studies revealed that pharmacologically, β‐ARs in many fish most closely resemble the mammalian β‐ARs (Dugan et al, ); therefore, the heart rate modulation could be directly linked to the intrinsic characteristics of β‐blockers. Additionally, our data suggested that the heart rate was the most sensitive endpoint parameter measured in the embryo developmental test.…”

Section: Discussionmentioning

confidence: 99%

Toxicity and enantiospecific differences of two β-blockers, propranolol and metoprolol, in the embryos and larvae of zebrafish (Danio rerio)

et al. 2013

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The risk presented by β-blockers on aquatic organisms remains uncertain, particularly given the enantiospecific differences in toxicity of chiral β-blockers. In this study, the toxicity of two β-blockers, propranolol and metoprolol, was determined. The 96-h LC50 of propranolol in the zebrafish larvae was 2.48 mg/L, whereas 50 mg/L metoprolol did not result in death. Both β-blockers decreased the heart rate and hatching rate and increased the mortality of the zebrafish embryos. Among these indicators, the heart rate was the most sensitive. However, the acute larval and embryo toxicity results displayed no enantioselectivity. Additionally, the transcriptional response of the genes encoding the β-adrenergic receptors and those involved in other physiological processes, including the antioxidant response, detoxification, and apoptosis, in zebrafish larvae exposed to the β-blockers was examined. Although the changes in gene transcription were fairly minor, significant enantioselectivity was observed for β-blockers, suggesting that the transcriptional response was more sensitive for the evaluation of enantiospecific toxicity. Based on these results, the pharmaceutical drugs were not expected to pose a risk to fish; however, this conclusion should not be considered final. These results also demonstrated that the enantiospecific toxicity of chiral β-blockers should be investigated when performing an ecological risk assessment.

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“…It would be interesting to further investigate the potential sub-function of each of these 2ARs in zebrafish, both in the heart and in other tissues where 2AR function is critical, such as the liver (e.g. Dugan et al, 2008).…”

Section: 2armentioning

confidence: 99%

In vivo and in vitro assessment of cardiac β-adrenergic receptors in larval zebrafish (Danio rerio)

Steele

Yang

Debiais-Thibaud

et al. 2011

Journal of Experimental Biology

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SUMMARY β-Adrenergic receptors (βARs) are crucial for maintaining the rate and force of cardiac muscle contraction in vertebrates. Zebrafish (Danio rerio) have one β1AR gene and two β2AR genes (β2aAR and β2bAR). We examined the roles of these receptors in larval zebrafish in vivo by assessing the impact of translational gene knockdown on cardiac function. Zebrafish larvae lacking β1AR expression by morpholino knockdown displayed lower heart rates than control fish, whereas larvae deficient in both β2aAR and β2bAR expression exhibited significantly higher heart rates than controls. These results suggested a potential inhibitory role for one or both β2AR genes. By using cultured HEK293 cells transfected with zebrafish βARs, we demonstrated that stimulation with adrenaline or procaterol (a β2AR agonist) resulted in an increase in intracellular cAMP levels in cells expressing any of the three zebrafish βARs. In comparison with its human βAR counterpart, zebrafish β2aAR expressed in HEK293 cells appeared to exhibit a unique binding affinity profile for adrenergic ligands. Specifically, zebrafish β2aAR had a high binding affinity for phenylephrine, a classical α-adrenergic receptor agonist. The zebrafish receptors also had distinct ligand binding affinities for adrenergic agonists when compared with human βARs in culture, with zebrafish β2aAR being distinct from human β2AR and zebrafish β2bAR. Overall, this study provides insight into the function and evolution of both fish and mammalian β-adrenergic receptors.

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Regulation of the black bullhead hepatic β-adrenoceptors

Cited by 16 publications

References 32 publications

Zebrafish β-adrenergic receptor mRNA expression and control of pigmentation

Zebrafish β-adrenergic receptor mRNA expression and control of pigmentation

Toxicity and enantiospecific differences of two β-blockers, propranolol and metoprolol, in the embryos and larvae of zebrafish (Danio rerio)

In vivo and in vitro assessment of cardiac β-adrenergic receptors in larval zebrafish (Danio rerio)

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