Regulation of the IL-10/IL-12 axis in human dendritic cells with probiotic bacteria

Abstract

In this study, we have used monocyte-derived dendritic cells (DCs) to design a screening model for the selection of microorganisms with the ability to suppress DC-secreted IL-12p70, a critical cytokine for the induction of T-helper cell type 1 immune responses under inflammatory conditions. By the treatment of DCs with cocktails containing TLR agonists and proinflammatory cytokines, the cells increased the secretion of the Th1-promoting cytokine IL-12p70. Clinically used probiotics were tested for their IL-10- and IL-12p70-stimulating properties in immature DCs, and showed a dose-dependent change in the IL-10/IL-12p70 balance. Lactobacillus acidophilus NCFM(™) and the probiotic mixture VSL#3 showed a strong induction of IL-12p70, whereas Lactobacillus salivarius Ls-33 and Bifidobacterium infantis 35624 preferentially induced IL-10. Escherichia coli Nissle 1917 induced both IL-10 and IL-12p70, whereas the probiotic yeast Saccharomyces boulardii induced low levels of cytokines. When combining these microorganisms with the Th1-promoting cocktails, E. coli Nissle 1917 and B. infantis 35624 were potent suppressors of IL-12p70 secretion in an IL-10-independent manner, indicating a suppressive effect on Th1-inducing antigen-presenting cells. The present model, using cocktail-stimulated DCs with potent IL-12p70-stimulating capacity, may be used as an efficient tool to assess the anti-inflammatory properties of microorganisms for potential clinical use.

The probiotic activity of Lactobacillus murinus against food allergy

“…Several probiotic bacteria, such as LAB, are known to affect the functionality of dendritic cells (DC) in culture (Gad et al, 2011). Modulatory effects on DC have been postulated as a critical mechanism for probiotic-mediated immune modulation (Amar et al, 2015;Dongarrà et al, 2013;Fong, Kirjavainen, Wong, & El-Nezami, 2015;Fong, Shah, Kirjavainen, & El-Nezami, 2015;Martínez-Abad et al, 2016;Yang et al, 2015).…”

Intestinal barrier: A gentlemen’s agreement between microbiota and immunity

“…Other studies have confirmed this immunomodulatory effect of Lactobacillus, showing that it leads to augmentation of IL-10 levels [129,132,133] and to a reduction of pro-inflammatory cytokines such as IL-6 and TNF-α [134] . Other probiotics are able to inhibit NF-κB, such as L. plantarum, suggesting that it induces tolerance to food antigens [135] .…”

Preparation of Complexes between Ovalbumin Nanoparticles and Retinoic Acid for Efficient Induction of Tolerogenic Dendritic Cells

“…As shown in Fig 6 C, the secretion of inflammatory cytokine IL-12p70 was suppressed by OVA/RA NPs comparing with the non-treated immature DC 2.4, while the secretion of anti-inflammatory cytokines IL-10 and TGF-β was kept unchanged. As a result, ratio of expression of IL-10/IL-12p70, which is used as a measure of anti-inflammatory response of cells, [28][29][30] was raised by OVA/RA NPs (Fig. 6 D).…”