Regulation of the prepronociceptin gene and its effect on neuronal differentiation

Nociceptin and Meiosis during Spermatogenesis in Postnatal Testes

“…None could be detected from immunoprecipitates with an unrelated antibody. Nucleotide sequencing of the detected DNA fragment confirmed the presence of a consensus CRE sequence (CGTCA) at 30 bp upstream of the ATG translation start codon in the proximal promoter of murine prepronociceptin gene as reported (Zaveri et al, 2006). These results indicated that phosphorylated CREB associates with the proximal promoter region of prepronociceptin gene in Sertoli B cells ( Fig.…”

“…We revealed that Sertoli B cells exhibit the same physiological responses to FSH via cAMP in at least PKA activation and CREB phosphorylation/activation as testicular Sertoli cells excluding the undetectable level of FSH receptor expression. Our ChIP assay demonstrated that cAMP/PKA pathway may regulate the association of phosphorylated CREB with a putative consensus CRE sequence in the proximal promoter of murine prepronociceptin gene, which seems to be highly conserved between Sertoli cells in testes and neurons in brains (Zaveri et al, 2002(Zaveri et al, , 2006. Moreover, we showed for the first time by RT-PCR analyses of Sertoli B cells and testes and Western blot analyses of peptide extracts from testes that the expressions of prepronociceptin mRNA and the nociceptin peptide may be upregulated by FSH signaling via cAMP/PKA/CREB pathway in Sertoli cells.…”

“…After cells were stimulated for 10 min with db-cAMP, extracts were prepared and processed for ChIP with the same antibody to phosphorylated CREB. We screened by PCR several genes, whose proximal promoter regions associate with phosphorylated CREB, and investigated murine prepronociceptin gene (Zaveri, Waleh, & Toll, 2006). The proximal promoter of murine prepronociceptin gene has one functional CRE site in a different location from the human promoter (Zaveri, Green, Polgar, Huynh, & Toll, 2002;Zaveri et al, 2006).…”

“…We screened by PCR several genes, whose proximal promoter regions associate with phosphorylated CREB, and investigated murine prepronociceptin gene (Zaveri, Waleh, & Toll, 2006). The proximal promoter of murine prepronociceptin gene has one functional CRE site in a different location from the human promoter (Zaveri, Green, Polgar, Huynh, & Toll, 2002;Zaveri et al, 2006). The DNA fragment from the putative transcription start site to the ATG translation start codon (252 bp) was detected only in cells treated with db-cAMP but not in untreated cells.…”

Functional antagonism between nociceptin/orphanin FQ (N/OFQ) and corticotropin-releasing factor (CRF) in the rat brain: evidence for involvement of the bed nucleus of the stria terminalis

“…The mechanism(s) underlying the increase in NOP mRNA in the BST remains unexplored. Normally, the human mRNA expression regulation pattern consists of a promoter of the N/OFQ gene, which contains two apparent CRE consensus elements for cyclic AMP stimulation and a Sp1 site, while the mouse and rat promoter has one putative CRE site (Zaveri et al 2006). The transcription and alternative splicing of human NOP receptor are controlled by two cell-specific alternate promoters lacking a TATA-box and rich in GC (Ito et al 2000).…”