Regulation of the protein-loading capacity of hydroxyapatite by mercaptosuccinic acid modification

Ishihara, shinobu; Matsumoto, Takuya; Onoki, Takamasa; Uddin, Mohammad Hafiz; Sohmura, Taiji; Nakahira, Atsushi

doi:10.1016/j.actbio.2009.10.019

Cited by 25 publications

(19 citation statements)

References 31 publications

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“…The amount of adsorbed cytochrome C (basic protein) increased from 25 to 55 mg mg -1 when mercaptosuccinic acid (MA) was immobilized onto HA [14]. However, the adsorption of BSA to MA-functionalized HA was significantly suppressed.…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, the binding of cytochrome c was significantly reduced. In another study, Ishihara et al [14] introduced MA, which contains two -COO 2 groups, onto HA to increase the loading capacity of HA for basic proteins.…”

Section: Protein Adsorption On Amino Acid-functionalized Hydroxyapatitementioning

confidence: 99%

“…However, the adsorption of BSA to MA-functionalized HA was significantly suppressed. This could be owing to the increase of negatively charged sites on HA surfaces once MA is immobilized on its surface [14]. Citrate is another candidate that could induce the electronegative potential of HA.…”

Section: Introductionmentioning

confidence: 99%

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Modulating protein adsorption onto hydroxyapatite particles using different amino acid treatments

Lee

Loo

Van

et al. 2011

J. R. Soc. Interface.

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Hydroxyapatite (HA) is a material of choice for bone grafts owing to its chemical and structural similarities to the mineral phase of hard tissues. The combination of osteogenic proteins with HA materials that carry and deliver the proteins to the bone-defective areas will accelerate bone regeneration. The study investigated the treatment of HA particles with different amino acids such as serine (Ser), asparagine (Asn), aspartic acid (Asp) and arginine (Arg) to enhance the adsorption ability of HA carrier for delivering therapeutic proteins to the body. The crystallinity of HA reduced when amino acids were added during HA preparation. Depending on the types of amino acid, the specific surface area of the amino acid-functionalized HA particles varied from 105 to 149 m 2 g -1. Bovine serum albumin (BSA) and lysozyme were used as model proteins for adsorption study. The protein adsorption onto the surface of amino acid-functionalized HA depended on the polarities of HA particles, whereby, compared with lysozyme, BSA demonstrated higher affinity towards positively charged Arg-HA. Alternatively, the binding affinity of lysozyme onto the negatively charged Asp-HA was higher when compared with BSA. The BSA and lysozyme adsorptions onto the amino acid-functionalized HA fitted better into the Freundlich than Langmuir model. The amino acid-functionalized HA particles that had higher protein adsorption demonstrated a lower protein-release rate.

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Section: Introductionmentioning

confidence: 99%

Section: Protein Adsorption On Amino Acid-functionalized Hydroxyapatitementioning

confidence: 99%

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Modulating protein adsorption onto hydroxyapatite particles using different amino acid treatments

Lee

Loo

Van

et al. 2011

J. R. Soc. Interface.

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“…19 Furthermore, the loaded macromolecules released completely from the biomaterials over a very short time, showing an initial burst release behavior. 20 The rhBMP-2 release experiments in this study showed that 75 wt% of rhBMP-2 was released from MCS-15 even after 14 days, obviously indicating sustained release of rhBMP-2 from MSC-15. However, more than 80 wt% of rhBMP-2 was released from MCS-4 within 3 days, indicating that the release of rhBMP-2 from MSC-4 was very quick, showing an initial burst release.…”

mentioning

confidence: 52%

Mesoporous calcium–silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2

Song

Qian

et al. 2015

IJN

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Mesoporous calcium–silicon xerogels with a pore size of 15 nm (MCS-15) and pore volume of 1.43 cm 3 /g were synthesized by using 1,3,5-mesitylene (TMB) as the pore-expanding agent. The MCS-15 exhibited good degradability with the weight loss of 50 wt% after soaking in Tris-HCl solution for 56 days, which was higher than the 30 wt% loss shown by mesoporous calcium–silicon xerogels with a pore size of 4 nm (MCS-4). The pore size and pore volume of MCS-15 had significant influences on load and release of recombinant human bone morphogenetic protein-2 (rhBMP-2). The MCS-15 had a higher capacity to encapsulate a large amount of rhBMP-2; it could adsorb 45 mg/g of rhBMP-2 in phosphate-buffered saline after 24 hours, which was more than twice that with MCS-4 (20 mg/g). Moreover, the MCS-15 system exhibited sustained release of rhBMP-2 as compared with MCS-4 system (showing a burst release). The MCS-15/rhBMP-2 system could promote the proliferation and differentiation of human mesenchymal stem cells, showing good cytocompatibility and bioactivity. The results indicated that MCS-15, with larger mesopore size and higher pore volume, might be a promising carrier for loading and sustained release of rhBMP-2, which could be used as bone repair material with built-in osteoinduction function in bone reconstruction.

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“…These particles derived from spherical amorphous CaP particles after aging (14 days) under ambient conditions. A high LSM adsorption capacity (i.e., up to 930 mg/g) was observed as well as a pH-sensitive release of adsorbed LSM [141].…”

Section: Protein Adsorption and Release From Hap Nanoparticlesmentioning

confidence: 87%

Biodegradable and Biocompatible Systems Based on Hydroxyapatite Nanoparticles

et al. 2017

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Abstract:Composites of hydroxyapatite (HAp) are widely employed in biomedical applications due to their biocompatibility, bioactivity and osteoconductivity properties. In fact, the development of industrially scalable hybrids at low cost and high efficiency has a great impact, for example, on bone tissue engineering applications and even as drug delivery systems. New nanocomposites constituted by HAp nanoparticles and synthetic or natural polymers with biodegradable and biocompatible characteristics have constantly been developed and extensive works have been published concerning their applications. The present review is mainly focused on both the capability of HAp nanoparticles to encapsulate diverse compounds as well as the preparation methods of scaffolds incorporating HAp. Attention has also been paid to the recent developments on antimicrobial scaffolds, bioactive membranes, magnetic scaffolds, in vivo imaging systems, hydrogels and coatings that made use of HAp nanoparticles.

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Regulation of the protein-loading capacity of hydroxyapatite by mercaptosuccinic acid modification

Cited by 25 publications

References 31 publications

Modulating protein adsorption onto hydroxyapatite particles using different amino acid treatments

Modulating protein adsorption onto hydroxyapatite particles using different amino acid treatments

Mesoporous calcium–silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2

Biodegradable and Biocompatible Systems Based on Hydroxyapatite Nanoparticles

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Regulation of the protein-loading capacity of hydroxyapatite by mercaptosuccinic acid modification

Cited by 25 publications

References 31 publications

Modulating protein adsorption onto hydroxyapatite particles using different amino acid treatments

Modulating protein adsorption onto hydroxyapatite particles using different amino acid treatments

Mesoporous calcium&ndash;silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of&nbsp;rhBMP-2

Biodegradable and Biocompatible Systems Based on Hydroxyapatite Nanoparticles

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Mesoporous calcium–silicon xerogels with mesopore size and pore volume influence hMSC behaviors by load and sustained release of rhBMP-2