Regulation of the renal proximal tubule second sodium pump by angiotensins

Caruso‐Neves, Celso; Rangel, L.B.A.; Lara, Lucienne S.; Lopes, Anibal Gil

doi:10.1590/s0100-879x2001000800015

Cited by 16 publications

(7 citation statements)

References 36 publications

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“…26). Experimental evidence suggests that Na ϩ -ATPase is a target for angiotensins and that it is increased in the kidneys of spontaneously hypertensive rats, without alterations in the expression of Na ϩ -K ϩ -ATPase (6,26). In view of these reports, future studies should investigate the potential role of AdeR on Na ϩ reabsorption in the kidney and intestines of different species.…”

Section: Discussionmentioning

confidence: 98%

Cellular localization of adenine receptors in the rat kidney and their functional significance in the inner medullary collecting duct

Kishore

Zhang

Gevorgyan

et al. 2013

American Journal of Physiology-Renal Physiology

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The Gi-coupled adenine receptor (AdeR) binds adenine with high affinity and potentially reduces cellular cAMP levels. Since cAMP is an important second messenger in the renal transport of water and solutes, we localized AdeR in the rat kidney. Real-time RT-PCR showed higher relative expression of AdeR mRNA in the cortex and outer medulla compared with the inner medulla. Immunoblots using a peptide-derived and affinity-purified rabbit polyclonal antibody specific for an 18-amino acid COOH-terminal sequence of rat AdeR, which we generated, detected two bands between ∼30 and 40 kDa (molecular mass of native protein: 37 kDa) in the cortex, outer medulla, and inner medulla. These bands were ablated by preadsorption of the antibody with the immunizing peptide. Immunofluorescence labeling showed expression of AdeR protein in all regions of the kidney. Immunoperoxidase revealed strong labeling of AdeR protein in the cortical vasculature, including the glomerular arterioles, and less intense labeling in the cells of the collecting duct system. Confocal immunofluorescence imaging colocalized AdeR with aquaporin-2 protein to the apical plasma membrane in the collecting duct. Functionally, adenine (10 μM) significantly decreased (P < 0.01) 1-deamino-8-d-arginine vasopressin (10 nM)-induced cAMP production in ex vivo preparations of inner medullary collecting ducts, which was reversed by PSB-08162 (20 μM, P < 0.01), a selective antagonist of AdeR. Thus, we demonstrated the expression of AdeR in the renal vasculature and collecting ducts and its functional relevance. This study may open a new avenue for the exploration of autocrine/paracrine regulation of renal vascular and tubular functions by the nucleobase adenine in health and disease.

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Section: Discussionmentioning

confidence: 98%

Cellular localization of adenine receptors in the rat kidney and their functional significance in the inner medullary collecting duct

Kishore

Zhang

Gevorgyan

et al. 2013

American Journal of Physiology-Renal Physiology

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“…It is worth mentioning that a variety of data in the field of proximal tubular reabsorption, lysosomal metabolism and intracellular processing have been collected during basic studies on the structure of kidney epithelial cells. The achievements have been summarized in several excellent reviews [109][110][111][112].…”

Section: Discussionmentioning

confidence: 99%

Renal Handling of Albumin—From Early Findings to Current Concepts

Gburek

Konopska

Gołąb

2021

IJMS

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Albumin is the main protein of blood plasma, lymph, cerebrospinal and interstitial fluid. The protein participates in a variety of important biological functions, such as maintenance of proper colloidal osmotic pressure, transport of important metabolites and antioxidant action. Synthesis of albumin takes place mainly in the liver, and its catabolism occurs mostly in vascular endothelium of muscle, skin and liver, as well as in the kidney tubular epithelium. Long-lasting investigation in this area has delineated the principal route of its catabolism involving glomerular filtration, tubular endocytic uptake via the multiligand scavenger receptor tandem—megalin and cubilin-amnionless complex, as well as lysosomal degradation to amino acids. However, the research of the last few decades indicates that also additional mechanisms may operate in this process to some extent. Direct uptake of albumin in glomerular podocytes via receptor for crystallizable region of immunoglobulins (neonatal FC receptor) was demonstrated. Additionally, luminal recycling of short peptides into the bloodstream and/or back into tubular lumen or transcytosis of whole molecules was suggested. The article discusses the molecular aspects of these processes and presents the major findings and controversies arising in the light of the research concerning the last decade. Their better characterization is essential for further research into pathophysiology of proteinuric renal failure and development of effective therapeutic strategies.

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“…Less widespread, the a-subunit of Na þ -ATPase was recently purified and cloned from the basolateral membrane of epithelial bowel of guinea pig [89]. This enzyme is responsible for approximately 10% of the reabsorption of filtered sodium [90] in the proximal tubule, and it is also designated as the ouabain-insensitive Na þ -ATPase transporter or a second sodium pump. Although recently cloned, the role of Na þ -ATPase in sodium reabsorption in the proximal tubule has been extensively investigated [91][92][93] and implicated in the development of hypertension in the spontaneously hypertensive rat (SHR) [89,94].…”

Section: Tubular Sodium Transporters and Hypertensionmentioning

confidence: 99%

How the Kidney Is Impacted by the Perinatal Maternal Environment to Develop Hypertension1

Paixão

Alexander

2013

Biology of Reproduction

120

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Environmental conditions during perinatal development such as maternal undernutrition, maternal glucocorticoids, placental insufficiency, and maternal sodium overload can program changes in renal Na(+) excretion leading to hypertension. Experimental studies indicate that fetal exposure to an adverse maternal environment may reduce glomerular filtration rate by decreasing the surface area of the glomerular capillaries. Moreover, fetal responses to environmental insults during early life that contribute to the development of hypertension may include increased expression of tubular apical or basolateral membrane Na(+) transporters and increased production of renal superoxide leading to enhanced Na(+) reabsorption. This review will address the role of these potential renal mechanisms in the fetal programming of hypertension in experimental models induced by maternal undernutrition, fetal exposure to glucocorticoids, placental insufficiency, and maternal sodium overload in the rat.

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Regulation of the renal proximal tubule second sodium pump by angiotensins

Cited by 16 publications

References 36 publications

Cellular localization of adenine receptors in the rat kidney and their functional significance in the inner medullary collecting duct

Cellular localization of adenine receptors in the rat kidney and their functional significance in the inner medullary collecting duct

Renal Handling of Albumin—From Early Findings to Current Concepts

How the Kidney Is Impacted by the Perinatal Maternal Environment to Develop Hypertension1

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