Regulation of the Thiobacillus intermedius glucose uptake system by thiosulfate

Romano, Antonio H.; Vranken, N J Van; Preisand, P; Brustolon, M

doi:10.1128/jb.121.2.577-582.1975

Cited by 9 publications

(3 citation statements)

References 20 publications

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“…For example, the metabolism of the two compounds may increase the concentration of a metabolite that inhibits the activity of the adipate uptake system. A possible basis for such an effect has been described recently by Romano et al (21), who showed that the glucose uptake system of Thiobacillus intermedius is inhibited by thiosulfate. It seems likely that the thiosulfate exerts its inhibitory effect by increasing the energy charge of the cells; the inhibiting metabolite may be either a high-energy compound like adenosine 5'-triphosphate or a compound that serves as an effective reductant for the electron transport chain (21).…”

Section: Discussionmentioning

confidence: 91%

“…A possible basis for such an effect has been described recently by Romano et al (21), who showed that the glucose uptake system of Thiobacillus intermedius is inhibited by thiosulfate. It seems likely that the thiosulfate exerts its inhibitory effect by increasing the energy charge of the cells; the inhibiting metabolite may be either a high-energy compound like adenosine 5'-triphosphate or a compound that serves as an effective reductant for the electron transport chain (21). It is conceivable that the inhibition of adipate transport in P. putida is due to a regulatory interaction among different transport systems.…”

Section: Discussionmentioning

confidence: 91%

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Properties of an inducible uptake system for beta-ketoadipate in Pseudomonas putida

Ornston¹,

Parke²

1976

J Bacteriol

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Wild-type strains of Pseudomonas putida form an inducible uptake system that appears to act on ,3-ketoadipate under normal physiological conditions. The system is induced by ,B-ketoadipate and is repressed by catabolites derived from it. Adipate is metabolized very slowly by wild-type P. putida cultures; [14C ]adipate was used as an analogue of ,B-ketoadipate to measure the transport activity in wild-type cells and in cells that constitutively produced the uptake system. Constitutive cells that contained high levels of the uptake system concentrated adipate to a level up to 200-fold above the concentration in the external medium. The process was energy dependent. The activity of the system with radioactive adipate was inhibited by ,8-ketoadipate, by 8-ketoadipate analogues, and by some compounds (e.g., acetate, glucose) that are structurally unrelated to ,8-ketoadipate; it is not known if the inhibitory effects are exerted directly by the compounds themselves or indirectly by catabolites derived from the compounds. The discovery of the ,8-ketoadipate uptake system is surprising in view of earlier studies that had indicated that ,3-ketoadipate does not permeate the membrane of wild-type P. putida cells. Contradictions between the former investigations and the present analysis are due primarily to the relatively high concentrations of substrate used in the earlier experiments. The existence of the ,-ketoadipate uptake system indicates that ,8-ketoadipate may exist as a selective nutrient in the natural niche of P. putida and may play a determinative role in the evolution of induction mechanisms that are characteristic of fluorescent pseudomonads.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 91%

Properties of an inducible uptake system for beta-ketoadipate in Pseudomonas putida

Ornston¹,

Parke²

1976

J Bacteriol

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“…Growth inhibition was always accoinpanied by the intracellular accumulation of elemental sulfur from either sulfide or thiosulfate. The mechanism of growth inhibition remains unknown, although an inhibition of sugar uptake by thiosulfate like in Thiobacillus intermedius may be possible (ROMANO et al 1975).…”

Section: Discussionmentioning

confidence: 99%

Metabolism of fructose in Thiocapsa roseopersicina

Conrad

Schlegel

1978

J of Basic Microbiology

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Thiocapsa roseopersicina strain 631 1 grew phototrophically in a mineral medium containing fructose as sole electron donor and carbon source with a doubling time of 11 -13 h, provided the mineral medium contained vitamine B,, (50 ng/ml), not more than 20 mM phosphate, and the culture was preincubated in the dark for 24 h. I n fructose-grown cells but not in autotrophically grown cells, the cell-protein content was strongly reduced when vitamine B,, was growth limiting, while the carbohydrate content was increased. Growth on fructose as sole carbon source was inhibited by the addition of sulfide or thiosulfate; growth inhibition was relieved in the presence of bicarbonate. No growth on fructose was observed anaerobically in the dark; aerobic growth in the dark was poor. Analysis of enzyme activities in fructose-and acetate-grown cells indicated that fructose was catabolized via fructose-1-phosphate and the EMBDEN-MEYERHOF pathway. The operation of the EMBDEN-MEYERROF pathway was confirmed by incorporation of lJC-, 3-I4C-, and 6-W-fructose into the spirilloxanthin fraction and analysis of its specific radioactivity.Many strains of Chromatiaceae have been tested for their ability to grow on glucose or fructose as electron donor (THIELE 1968a, MATHERON and BAULAIGUE 1972), but only few turned out to utilize one of these hexoses. Thiocapsaroseopersicina is the sole species which was repeatedly found to utilize either fructose (THIELE 1968a, MATHERON and BAULAIGUE 1972) or glucose (MATHERON and BEAULAIGUE 1972, BOGOROV 1974).I n a carbohydrate-rich sewage plant even mass development was reported for this bacterium (HOLM and VENNES 1970). However, among all the other species of the Chromatiaceae sugar utilization occurs only in few strains ; sugar utilization has been reported for strains of Chromatium vinosum (KONDRATIEVA 1963, THIELE 1968a. Thiocystis violacea, Amoebobacter roseus, and A . pendens (THIELE 1968a) and Ectothiorhodospira mobilis (TRUPER 1968). I n these strains the sugars are believed to be utilized as both, electron donor and carbon source, since the growth yield is exceedingly high in a sugar supplemented medium which contains carbonate and some sulfide as the only other carbon source and electron donor.Although the glucose and fructose catabolic pathway shave thoroughly been studied in species of the Rhodospirillaceae, as in Rhodospirillum rubrum ( GIBSON and WANG 1968, SCHON andBIEDERMANN 1972), Rhodopseudomonm capsulata ( EIDELS and PREISS 1970, CONRAD and SCHLEGEL, 1974,1977a and R . sphaeroides (SZYMONA andDouDoRoFF 1960, OHMANN et at?. 1969, 1970, CONRAD and SCHLEGEL 1974, 1977b, the information concerning sugar catabolic pathways in species of the Chromatiaceae is scarce ( SCHLEGEL 1974, KRASILNIKOVA 1975).In the present paper we studied the growth of T.roseopersicina strain 6311 on fructose and the influence of the addition of bicarbonate, vitamine B,,, and thiosulfate on the growth response. The fructose catabolic pathway was investigated by means of enzyme analysis and radiolabelling of sp...

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Metabolism of fructose inThiocapsa roseopersicina

Conrad¹,

Schlegel²

1978

Z Allg Mikrobiol

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Thiocapsa roseopersicina strain 6311 grew phototrophically in a mineral medium containing fructose as sole electron donor and carbon source with a doubling time of 11--13 h, provided the mineral medium contained vitamine B12 (50 ng/ml), not more than 20 mM phosphate, and the culture was preincubated in the dark for 24 h. In fructose-grown cells but not in autotrophically grown cells, the cell-protein content was strongly reduced when vitamine B12 was growth limiting, while the carbohydrate content was increased. Growth on fructose as sole carbon source was inhibited by the addition of sulfide or thiosulfate; growth inhibition was relieved in the presence of bicarbonate. No growth on fructose was observed anaerobically in the dark; aerobic growth in the dark was poor. Analysis of enzyme activities in fructose- and acetate-grown cells indicated that fructose was catabolized via fructose-1-phosphate and the EMBDEN-MEYERHOF pathway. The operation of the EMBDEN-MEYERHOF pathway was confirmed by incorporation of 1-14C-, 3-14C-, and 6-14C-fructose into the spirilloxanthin fraction and analysis of its specific radioactivity.

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Regulation of the Thiobacillus intermedius glucose uptake system by thiosulfate

Cited by 9 publications

References 20 publications

Properties of an inducible uptake system for beta-ketoadipate in Pseudomonas putida

Properties of an inducible uptake system for beta-ketoadipate in Pseudomonas putida

Metabolism of fructose in Thiocapsa roseopersicina

Metabolism of fructose inThiocapsa roseopersicina

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